Addgene

We narrowed to 4 results for: CAG

Showing: 1 - 4 of 4 results

Plasmid Modification by Annealed Oligo Cloning (with Protocols)

Type

Protocol

...AATTC and G - 3' to the top oligo and 3' - G and CAGCT - 5' to the bottom oligo, making our final oligos...Bottom oligo: 3' - G GTATAC AATTAATT CCGCGCGG GTTAAC CAGCT - 5' Note: We could leave off the 3’ G on each oligo...
Plasmid Cloning by PCR (with Protocols)

Type

Protocol

...final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Experimental Procedure Run PCR...
AAV Titration by qPCR Using SYBR Green Technology

Type

Protocol

...'-GGAACCCCTAGTGATGGAGTT rev ITR primer, 5'-CGGCCTCAGTGAGCGA ITR-containing plasmid for standard curve ...
AAV ddPCR Titration

Type

Protocol

...probe targeting ITR: ITR Forward Primer: 5’-CGGCCTCAGTGAGCGA ITR Reverse Primer: 5’-GGAACCCCTAGTGATGGAGTT...

Showing: 1 - 4 of 4 results