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Showing: 41 - 60 of 82 results

  1. Plant Plasmids and Resources

    Type
    Collection
    ...Description Cloning Type PI 1000000147, 1000000148, 1000000149, 1000000150 2in1 Plasmid Toolkit Plasmids...Lenaghan 1000000241 GreenGate 2.0 Toolkit Compatible with GreenGate Cloning System (#1000000036). Includes... Voytas 1000000241 GreenGate 2.0 Toolkit Compatible with GreenGate Cloning System (#1000000036). Includes...expression vectors. ID Kit Description Cloning Type PI 1000000036 GreenGate Cloning System Plasmids for rapidly...Gate method. Golden Gate (GreenGate) Jan Lohmann 1000000076 GoldenBraid 2.0 Kit Plant synthetic biology kit...parts. Golden Gate (GoldenBraid) Diego Orzaez 1000000135 MoClo Plant Parts II and Infrastructure Kit Modules...GoldenBraid. Golden Gate (MoClo) Johannes Stuttmann 1000000233 Joint Modular Cloning (JMC) Toolkit Modular cloning...

  2. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...filter, VWR 431229 0.22 µm PES filtering system, 1000 mL, VWR 431098 0.45 µm PES filtering system, 500... aliquots and store at -20 °C until use. BCD TFX 1000 mL BalanCD HEK293 Media 10 mL 10% Poloxamer 188 ...suggest preparing fresh solutions after one month. 1000X protease inhibitor cocktail 25 mg Leupeptin 50 mg...

  3. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...36857M 100038-AAV1 pENN.AAV.hSynap.Flex.ArcLightDco.WPRE.SV40 Vincent Pieribone AV-1-36857P 100037-AAV1 ...Looger AV-1-ALL856 100048-AAV1 pAAV.CAG.LSL.tdTomato Hongkui Zeng AV-9-ALL856 100048-AAV9 pAAV.CAG.LSL....Penn ID Addgene ID Name Function PI AV-1-49531P 100040-AAV1 pAAV.hSyn1.Twitch2B.WPRE.SV40 Biosensor Oliver...AAV-EF1a-BbChT Control Joshua Sanes AV-9-PV2629 100043-AAV9 pAAV.synP.DIO.EGFP.WPRE.hGH Control Ian Wickersham...-HGHpA Optogenetics Karl Deisseroth AV-1-20938M 100054-AAV1 pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 Optogenetics...3.0-EYFP Optogenetics Karl Deisseroth AV-1-26967M 100049-AAV1 pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH...-HGHpA Optogenetics Karl Deisseroth AV-5-20938M 100054-AAV5 pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 Optogenetics...

  7. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...Drawbacks. Clon Transgen 4:138. doi:10.4172/2168-9849.1000138 2. Hartley JL. Use of the Gateway System for...

  9. Bacterial Expression Systems

    Type
    Collection
    ...Consortium Modular Assembly Kits (Addgene #1000000005, 1000000010) contain plasmids and an engineered strain...localization to the periplasm. The pCri System (Addgene #1000000058) plasmids can be utilized for heterologous cytoplasmic...pastoris . The EcoFlex MoClo Toolkit (Addgene #1000000080) features a library of promoters and purification... cpFRET - FRET-based Biosensors Kit (Addgene #1000000021) is a vector library for the generation of FRET...ratiometric measurements. The MoBiFc Toolkit (Addgene #1000000222) is a collection of Golden Gate compatible vectors... repression. The BIOFAB plasmid set (Addgene #1000000037) contains specific bacterial promoters that vary... . The Marionette Sensor Collection (Addgene #1000000137) allows you to independently control the expression...

  10. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette Pipette controller Microcentrifuge...Pipette tips, 200 µL, VWR 76322-150 Pipette tips, 1000 µL, VWR 76322-154 Pipettes, 5 mL, VWR 89130-896 ...

  11. Western Blot

    Type
    Protocol
    ...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette Pipette controller Pipette...your cell pellet but will generally be between 250–1000 µL . The ideal lysis buffer will vary depending ...

  15. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...Electrophoresis. 2010 Jul;31(14):2436-41. doi: 10.1002/elps.201000061. PMID: 20589859. Levene SD. Analysis of DNA topoisomers...

  17. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette 20–200 µL multichannel...Microcentrifuge tubes, Neptune 3745.X Pipette tips, 1000 µL, VWR 76322-154 Pipette tips, 10 µL, VWR 76322...

  18. Optogenetics AAV Preps

    Type
    Collection
    ...ChR2/H134R GFP Constitutive 8, rg* Edward Boyden 100054 pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40 CAG ChR2...ChrimsonR tdTomato Flp dependent 8 Edward Boyden 100049 pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH Syn...Syn ChETA EYFP Constitutive 1, 9 Karl Deisseroth 100050 pAAV.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH CaMKII...Constitutive 1, 5, 9, PHPeB Jordane Dimidschstein 100061 pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry EF1a C1V1 (...

  19. AAV ddPCR Titration

    Type
    Protocol
    ...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette 1–10 µL multichannel pipette...calculate the titer for each sample: $$T = {R*C*D*1000\over V}$$ Where: T = Titer, GC/mL R = Reaction volume...

  20. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ... Zinc Finger Consortium: OPEN Reagents (Kit # 1000000013 ) Depositing Labs: Keith Joung This kit consists...publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your Reference section: Oligomerized pool...
Showing: 41 - 60 of 82 results