...transactivator (tTA), is injected at a high dose to maximize the number of cells that are labeled. Part 2: ...

...database. They successfully edited 33/34 sites with a maximum of 28% editing efficiency in HEK293T cells, as ...

...Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

...with minimal side-effects on healthy cells and maximal effect on unhealthy cells. Until recently, the ...

...negotiating our energy and attention. The goal is to maximise the time you are enjoying both work and personal...

...large edits with similar or better efficiency than PEmax, the current state-of-the-art. PE6b was very efficient...

...from abasic sites. This approach resulted in a maximum A → T product purity of 66%. Pro tip! Abasic sites...

...in some cases, over 20-fold higher editing than PEmax. The authors hypothesized that the improved efficiency...

...contains one viral genome. Other than a theoretical maximum value, physical titers don’t necessarily give any...

... been used up. This ensures you can retain the maximum stocks and use of the original Cell line you purchased...

...a gene, you should design a control that shows maximal activation. Likewise if you are trying to repress...

...choosing a complementary pair of fluorophores. To maximize the FRET signal you should choose the highest ...

...Science Part: Democratic values and their role in maximizing the objectivity of science.  Scientific American...

...1998). The deprotonated state has an absorbance maximum around 488 nm and it emits light that peaks at ...

...The numbers in (e)miRFP names correspond to the maxima of their emission spectra. These probes can be ...

...Importantly, by varying these ratios, you can either maximize or minimize expression levels of your gene of ...

...quantities of DNA, you’ll need to do a midiprep or a maxiprep. In these purifications, you generally lyse the...

...and are described in Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9...

...early on. Once your system is working, you should maximize data production with the end goal of having sufficient...

...mammalian cells without decreased efficiency, with a maximum cargo capacity of ~200 kB of DNA. Similar to piggyBac...

...cells were transfected in 96-well format using an Amaxa Shuttle device; 5 days after the transfection, the...

...FRET acceptor. These experiments will define the maximum and minimum energy transfer values you can expect...

...duplex, and it’s the reason guide sequences have a maximum length of 20 bases: nothing longer will fit. Stabilization...

...probabilistically ON or OFF following Cre recombination to maximize label diversity Mammalian Megason 13769 pCALNL-DsRed...

... further optimization can be performed to both maximize the separation and reduce any background staining...

... a structured motif to enhance pegRNA stability PEmax - optimized PE architecture; increased synergy between.... PMID: 25079318 Bondy-Denomy, J., Pawluk, A., Maxwell, K. L., & Davidson, A. R. (2013). Bacteriophage...Rollins, M. F., Hidalgo-Reyes, Y., Wiedenheft, B., Maxwell, K. L., & Davidson, A. R. (2015). Multiple mechanisms...Kranzusch, P. J., Sontheimer, E. J., Davidson, A. R., Maxwell, K. L., & Doudna, J. A. (2017). A Broad-Spectrum..., J., Edraki, A., Shah, M., Sontheimer, E. J., Maxwell, K. L., & Davidson, A. R. (2016). Naturally occurring...

...that antibodies may need longer to reach their maximum binding, so feel free to trial even longer incubation...

...either case, next-generation sequencing of the maxiprep DNA is recommended to verify that the library ...process. Amplify the library (electroporation and maxiprep). If delivering as virus, make virus; this creates...

...your substance maximally absorbs. In the case of nucleic acid (DNA and RNA), the maximal absorbance is ...and most have a specific wavelength that they maximally absorb. By measuring the absorbance of a liquid...is at 260nm. Protein maximally absorbs at 280nm and the ratio of nucleic acid to protein (260/280) is generally...

...and on which you can individually distinguish a maximum of ~100 colonies Pro-Tip If you will be screening...like to make 20 plates, and our plates can hold a maximum of 10 mL, we’ll want 200 mL of media total. Importantly...

... it is also important to consider factors that maximize cleavage of the desired target sequence or on-target...

...primer should be a minimum of 50 nucleotides and a maximum of 300 nucleotides from your target. Are there ...

...fill the water bath, and be sure to know what the maximum fill capacity is. Look for a “fill” line inside...

... might hold a pipette. Without moving past the maximum possible setting on the pipette, set the desired...

...this protocol could, in theory, give rise to a maximum of 48 monoclonal cell lines. When this protocol...

...but do not stick to PES. Using a PES filter will maximize titer . DMEM high glucose, Corning 10-013-CV DMEM...

...receptor AIS, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM AREG amphiregulin AR, CRDGF, MGC13647, SDGF...

...with AgeI. Mix: 6 μg pLKO.1 TRC-cloning vector (maxiprep or miniprep DNA) 5 μL 10x NEB buffer 1 1 μL AgeI...