...store the solution at -20 °C. 1 mg/mL PEI-MAX Add 1 g of PEI-MAX powder to 900 mL deionized water in a 1... mg/mL PEI-MAX to the second tube of 6 mL BCD TFX (for a 1 mg/mL stock solution of PEI-MAX, 450 µg = 450...recombinant antibody plasmids using Polyethylenimine Max as a transfection reagent. After transfection and... Polyethylenimine hydrochloride, M.W. 40000 (PEI-MAX), Linear, Transfection Grade, VWR 75800-188 10% Pluronic...Before Starting Warm the DNA and working stock of PEI-MAX to room temperature before use, and warm the BalanCD...for 1 h in the 37 °C bead bath. Transfer the PEI-MAX and recombinant antibody plasmid DNA sample to the...ratios may range from 1:1 to 1:6. Add the diluted PEI-MAX to the diluted DNA. Cap the tube and vortex with ...

...chloroquine. After the incubation, add the DNA:PEI-Max mix to the conical containing the media and chloroquine...

...65 mm Petri dish (VWR, 11019-552) 4 MaxiPreps (Qiagen HiSpeed Max, Catalog #12663) Tips (1000 µL, 200 ... for the purpose of a Maxiprep. Purify plasmid DNA using the Qiagen HiSpeed Maxi Kit (one conical is its...representation is maintained. Maxipreps - Less is more: Do not overload the Maxipreps as yield can dramatically...spread out the harvested cells further than four Maxipreps worth. Count colonies on the most dilute Petri... its own Maxiprep). Critical Do not freeze pellets for later purification. Immediately purify them! Commercial...Commercial Maxipreps rely on incremental, ordered cell lysis. E. coli cells are subject to lysis by freeze-thaw...Glycerol or DMSO solutions. Quantify the individual Maxipreps by Nanodrop, Picogreen, or Qubit. Each different...

...your substance maximally absorbs. In the case of nucleic acid (DNA and RNA), the maximal absorbance is ...and most have a specific wavelength that they maximally absorb. By measuring the absorbance of a liquid...is at 260nm. Protein maximally absorbs at 280nm and the ratio of nucleic acid to protein (260/280) is generally...

...and on which you can individually distinguish a maximum of ~100 colonies Pro-Tip If you will be screening...like to make 20 plates, and our plates can hold a maximum of 10 mL, we’ll want 200 mL of media total. Importantly...

...primer should be a minimum of 50 nucleotides and a maximum of 300 nucleotides from your target. Are there ...

...fill the water bath, and be sure to know what the maximum fill capacity is. Look for a “fill” line inside...

... might hold a pipette. Without moving past the maximum possible setting on the pipette, set the desired...

...this protocol could, in theory, give rise to a maximum of 48 monoclonal cell lines. When this protocol...

...but do not stick to PES. Using a PES filter will maximize titer . DMEM high glucose, Corning 10-013-CV DMEM...

...with AgeI. Mix: 6 μg pLKO.1 TRC-cloning vector (maxiprep or miniprep DNA) 5 μL 10x NEB buffer 1 1 μL AgeI...