... expert (SME) at eScience Labs, an educational company that creates hands-on science lab kits and digital...with faculty members to make sure the courses encompass what they want to teach.” Besides working with...lab: write!” Caitlin did this by editing 15+ comprehensive exam proposals, a few PhD theses, and occasionally...

...information.” Addgene is here to help scientists accomplish their goals. Many nonprofits must spend a lot...level of positive revenue to build reserves so the company is sustainable. The difference is what nonprofits...-oriented values. Addgenies like the nonprofit company culture as well. We work every day to do a better...

... life science researchers. Many biotechnology companies are looking for PhD or Master’s level scientists...have done?” and “Would their academic experience compliment this sales and marketing role?”. Studying for...entrepreneurial bug and has set up an ELISA kit company on whose website you can find some great information...

...of their luciferase assay, they concluded that components in the buffer used for protein purification must...percentages (78-79% after two rounds of transduction.) Compared to CCP transduction, iTOP is at least four times...Astolfo et al. were eager to see if iTOP would be compatible with CRISPR/Cas9 genome editing. Although CRISPR...

...faithfully transcribes RNA to DNA with low error rates compared to other RTs. MarathonRT represents a major step...long-read cDNA sequencing and RNA sequencing of complex heterogeneous RNA samples. Furthermore, such high... restricted spatial tagging), to confirm the composition of proteins found at the telomere, and hope it...

...used live virus (Cele et al., 2021). “We weren't completely shocked [with these results] because we were ... variant, which has only 9 different mutations compared to the wild-type, escaped neutralization. “That...SARS-CoV-2 variants, this study was the first to compare so many different strains. It was also the first...

...common lexicon for genome editing studies. These complementary goals are aimed at improving translation of ... loosely paraphrased in the tweet below. The complexity and challenges of homology directed repair (HDR... I think I may have finally grasped just how complicated DNA repair pathways are. I fully admit to being...

...experience using image analysis software. If you use computer programs to predict protein structures, then you... you might know a thing or two about computer modeling. Make sure to focus on these skills. The only exception... into a communications role instead after she completed her MSc in Experimental Medicine. She is the Community...

...than a day. Diagnostic restriction digests are comprised of 2 separate steps: 1) incubating your DNA with... construct. Consider buffer and temperature compatibility when digesting with more than one enzyme. Consult...will allow you to approximate the DNA mass of comparably intense samples of similar size. Run the gel ...

... It doesn’t have to be this way. We can never completely eliminate the risk that a minor variation in ...Press.  She trained originally in cell biology, completing her PhD at the MRC LMB in Cambridge UK and then... Vice President. Her current responsibilities encompass overall leadership of the Cell Press editorial...

...binds to TIR1 of the SCF-TIR1 ubiquitin ligase complex, promoting its interaction with the degron sequence...used ChRs in that they cause a rapid and almost complete desensitization when exposed to continuous bright...snaking through our cells’ nuclei--our chromatin is comprised of thousands of loops. These loops not only provide...

... I asked participants to read short abstracts comparing the mortality rates between two controversial ...and biased. As a result, the control abstract comparing two fictional medical issues – chronic and acute...community. I brought along printed copies of studies comparing the filtration efficacy of different materials...

...responsible for the degradation of over 600 cellular components ultimately leading to cell fragmentation and ...FlipGFP. FlipGFP increases fluorescence 100-fold compared to other reporters (Zhang et al., 2019). How does...and (3) β-strand 11 (β11). Each part contains components and residues that are necessary for fluorescence...

...sources. While the importance of understanding these complex processes can’t be overstated, we can also learn...degradation under a variety of conditions. They are composed of 4 proteins encoded in a single operon and were...

...writing and this recent article published in PLOS Computational Biology (Frassl et al., 2018): “Science is increasingly...collaboratively writing a multi-authored paper. PLoS Comput Biol 14:e1006508 . https://doi.org/10.1371/journal.pcbi...Simple Rules for a Successful Collaboration. PLoS Comput Biol 3:e44 . https://doi.org/10.1371/journal.pcbi...

.... Generation of sgRNA libraries, with maximum complexity, is achieved with synthetic oligos with a randomized... genome. Many of these techniques require more complex DNA assemblies and improvements in these methodologies...the limitations associated with otherwise more complicated assembly approaches, and opens the door for new...

...biosensor with two components - an enzyme that breaks down the HMO of interest into its component simple sugars...learn more about Xin’s work with photoswitchable components of the ERK signaling pathway in our recent blog...

...to start a more formal mentoring program at my company, but there weren't enough senior people willing... such as pre-reads, videos or exercises to be completed in preparation for the meeting or to be read or... and help each other practice meeting people – compete for fastest to set up a coffee date or most cards...

...However, one of the many proteins which are not compatible with regular mesophilic systems are the psychrophilic...chaperones protein resulted in higher levels of growth compared to the E. coli strain expressing the empty vector... Medal in the Undergrad category at the iGEM Competition 2019. He has an active interest in scientific...

...that use reverse transcriptase to synthesize complementary DNA copies of their genome upon infecting a ...gamma-retrovirus are so closely related, their required components are very similar. While plasmids containing virus-specific...between the two systems, other, more general, viral components such as a heterologous envelope or a post-transcriptional...