We narrowed to 610 results for: gan

1. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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   ...drosophila, worms and mammals (Browse plasmids by model organism, here).  More information on the various delivery...

2. Hot Plasmids Spring 2024

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   ...vitro and in vivo, including in human retinal organoids and live mice. Importantly, REVeRT avoids the ...

3. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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   ...NGG VQR variant D1135V, R1335Q and T1337R NGAN or NGNG EQR variant D1135E, R1335Q and T1337R...

4. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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   ...enrich adult mouse hematopoietic stem cells. Its ligand(s) has not been identified. While LY6A or other...

5. New Tools Enable CRISPRa for Neuroscience Applications

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   ...applications require such optimized tools. This is elegantly demonstrated by the Qi Lab, which developed a ...

6. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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   ...gene gets its name based on what happens to an organism when a gene/protein is depleted in a knockout ...

7. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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   ...toxins to claim territory or kill competing microorganisms, but did you know bacteria also produce substances...

8. Viral Vectors 101: Pseudotyping

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   ...more safely. Recently, many SARS-CoV-2 researchers began pseudotyping the SARS-CoV-2 spike protein into a...

9. CRISPR-based Genome Editing Systems For Editing in E. coli

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   ...on the escape mechanism of genome editing in microorganisms which could be applied to optimization of genome...

10. Bioinformatics at Addgene

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    ... collection of scripts evolved into a single, organized pipeline and accelerated the processing of our...

11. Healthcare Consulting: A Door to the Business of Life Sciences

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    ...pharmaceutical companies, nonprofit or global health organizations, investment and equity research firms, biotech...

12. Adeno-associated virus (AAV) Guide

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    ...AAV vectors . Microorganisms, 12 (2), 384. https://doi.org/10.3390/microorganisms12020384 (Link opens in...genes. rAAV episomes can develop chromatin-like organization and persist in non-dividing cells for a period...optimal serotype(s) for transduction of a given organ. Tissue Optimal Serotype Central nervous system ...administration, distributing widely throughout the organism to target large or diffused biological structures... of systemic rAAV vectors that can target whole organs or specific cell types of the CNS (e.g. AAV-PHP.eB... transport Movement of molecules, vesicles or organelles from the main cell body, or soma, towards the...pathogen, like viruses or bacteria, within a host organism, leading to disease. Insertional mutagenesis Random...

13. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    ...versatile tool for precise gene editing in many organisms and model systems. We have used CRISPR/Cas9 extensively...

14. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    ...phenotype that can be used with mutational scanning: ligand binding assays, phage display assays, ubiquitination...

15. CRISPR 101: Homology Directed Repair

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    ...). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development 141; (2014). PubMed...

16. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    ...indispensable tools for manipulating cells and organisms. They can be used for a variety of research purposes...

17. Hot Plasmids: Summer 2024

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    ...science.ado7082.  Nuñez, J. K., Chen, J., Pommier, G. C., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss,...

18. Harnessing Bacterial Toxins for Allelic Exchange

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    ...s toolbox. Depending on the growth rate of the organism of interest, from the day primers arrive, the ...

19. Multiple Plasmids at a Low Price: Kits from Addgene

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    ...Sciences 108.38 (2011): 15870-15875. See Kendall Morgan’s blog post on the PUF Assembly Kit for some insights...

20. 10 Steps to a Perfect Science Talk

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    Blog Post

    ...Speaking longer just makes you seem unprepared or arrogant. A bit short is always better than too long, and...