We narrowed to 612 results for: gan

1. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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   Blog Post

   ...gene gets its name based on what happens to an organism when a gene/protein is depleted in a knockout ...

2. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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   Blog Post

   ...enrich adult mouse hematopoietic stem cells. Its ligand(s) has not been identified. While LY6A or other...

3. New Tools Enable CRISPRa for Neuroscience Applications

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   Blog Post

   ...applications require such optimized tools. This is elegantly demonstrated by the Qi Lab, which developed a ...

4. Hot Plasmids Spring 2024

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   Blog Post

   ...vitro and in vivo, including in human retinal organoids and live mice. Importantly, REVeRT avoids the ...

5. Antibodies 101: Reading a Flow Plot

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   ...Vegas, A., Ma, M., Sahay, G., Chiu, A., Bader, A., Langan, E., Siebert, S., Li, J., Greiner, D., & Anderson...

6. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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   Blog Post

   ...drosophila, worms and mammals (Browse plasmids by model organism, here).  More information on the various delivery...

7. Bioinformatics at Addgene

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   Blog Post

   ... collection of scripts evolved into a single, organized pipeline and accelerated the processing of our...

8. Viral Vectors 101: Pseudotyping

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   ...more safely. Recently, many SARS-CoV-2 researchers began pseudotyping the SARS-CoV-2 spike protein into a...

9. CRISPR-based Genome Editing Systems For Editing in E. coli

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   ...on the escape mechanism of genome editing in microorganisms which could be applied to optimization of genome...

10. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    Blog Post

    ...indispensable tools for manipulating cells and organisms. They can be used for a variety of research purposes...

11. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    ...versatile tool for precise gene editing in many organisms and model systems. We have used CRISPR/Cas9 extensively...

12. Multiple Plasmids at a Low Price: Kits from Addgene

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    Blog Post

    ...Sciences 108.38 (2011): 15870-15875. See Kendall Morgan’s blog post on the PUF Assembly Kit for some insights...

13. 10 Steps to a Perfect Science Talk

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    Blog Post

    ...Speaking longer just makes you seem unprepared or arrogant. A bit short is always better than too long, and...

14. Evolution of Lab Techniques

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    Blog Post

    ... abundance. If a scientist wants to know if an organism has a particular gene mutation, southern blotting...

15. Harnessing Bacterial Toxins for Allelic Exchange

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    ...s toolbox. Depending on the growth rate of the organism of interest, from the day primers arrive, the ...

16. Hot Plasmids: Summer 2024

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    ...science.ado7082.  Nuñez, J. K., Chen, J., Pommier, G. C., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss,...

17. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    ...phenotype that can be used with mutational scanning: ligand binding assays, phage display assays, ubiquitination...

18. CRISPR 101: Homology Directed Repair

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    ...). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development 141; (2014). PubMed...

19. How to Negotiate a Successful Lab Start Up Budget

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    Blog Post

    ...provide value to others This is a non-profit organization. Therefore, collaboration and community is the...

20. Plasmids 101: Common Lab E. coli Strains

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    Blog Post

    ...blog post covering E. coli and others are model organisms! Our Most Popular Plasmids 101 Posts: Origin...