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Showing: 561 - 580 of 882 results

  1. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...standard curves, and normal controls must be run in triplicate.  The nice thing about qPCR is that since it ... of reactions, it does not need to be run in triplicates. ddPCR is an extremely accurate application, ...

  2. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...This post was contributed by guest blogger Iris Lindberg, Professor at the University of Maryland School...Cloning cells using an agarose overlay does have one tricky step. It is necessary to pour the agarose onto ...

  4. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...are treated with a drug of interest and gRNA distribution is analyzed in the drug-resistant population...6 gRNAs per target gene, and maintaining the distribution of each gRNA within the population is key. Loss...

  5. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...This post was contributed by Jae Lee and Pantelis Tsoulfas of the Department of Neurological Surgery ...along the illumination path. The shadows appear as stripes in the acquired image (5).  A way to get around...

  6. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...multicistronic vectors work? Scientists have "borrowed" some tricks discovered in positive single-stranded RNA viruses...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...

  7. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...This post was contributed by Gal Haimovich of greenfluorescentblog. Be honest. Do you really know how... short as 100ms (EBFP) or as long as 1 hour (mAmetrine1.2). However, for most FPs it is a few seconds ...

  8. Tips for arabidopsis transformation

    Type
    Blog Post
    ...This post was contributed by Laura Lee, a graduate student at Stanford University. Arabidopsis is a fantastic... it pretty much is, but there are some tips and tricks you can use to obtain optimum transformation efficiency...

  9. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...’ve got you covered in this blog, with tips and tricks for harnessing the homology-directed repair pathway...are overly confluent or don’t have sufficient nutrients in their media, they will not undergo DNA replication...

  10. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...conditions commonly encountered in human gene therapy trials. Human Gene Therapy Methods, 26(2), 71–76. https...Qu, G. (2005). Identification of factors that contribute to recombinant AAV2 particle aggregation and ...

  11. An Introduction to Adenovirus

    Type
    Blog Post
    ...noticing unusual cases of acute hepatitis in the pediatric population. Once ~400 cases had amassed in places...removal of certain adenoviral genes that apparently contribute to virulence (Tollefson et al., 1996) – cell ...

  13. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...functions differently. Since Cpf1 loci are widely distributed across bacterial species, Zetsche et al. hypothesized...directional gene transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer...

  14. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ... This post was contributed by Jo Miller, founder and principal of J. Miller & Associates, Inc., a national...professionals is learning to succeed in grants through trial and error, through winning and losing funding, and...

  16. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...an elderly family member, want to train for a triathlon, finally buy that charming fixer-upper house you... every school performance, chaperone every field trip or cheer at every soccer game. Tip: take turns going...

  18. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...This post was contributed by Samuel Mortensen, a PhD candidate at Northeastern University. Working with... 5.02 (2003): 103-115. Hu, Ching-yeh, et al. "Intrinsic GUS-like activities in seed plants." Plant cell...

  20. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at the Janelia...mechanistic, doable by 1-3 people in 1-5 years, and contribute to resolving the overarching knowledge gap. Aims...
Showing: 561 - 580 of 882 results