...Feedback is help and it should result in authentic assistance for the recipient to become more effective and...successful manager. Great bosses tell people where they stand clearly and routinely. They are clear with each ...questions after a task or period of work. Using this standard format not only enables you to discuss a person's...looking for a new position. One of the most common mistakes I see is managers who hint at or use indirect ...Steven Pinker. As a manager you must be able to state your observations truthfully and in clear terms....

...community. In the long term, the research project establishes your professional brand. It offers colleagues... as a stepping stone into the next professional stage of your career by connecting you with people of ...plan? Have you read the relevant literature to understand where the field is today? Have you talked to ...to use to reach these milestones? Can you get assistance from people who have the required expertise to...Choosing a Research Project Advice for Scientists Starting a Lab    ...

...expression, is it necessary to add a G nucleotide to the start of my target sequence? A2: The human U6 promoter...promoter prefers a 'G' at the transcription start site to have high expression, so adding this G could help ...when they order the oligo. If your spacer sequence starts with a 'G', you naturally have one and do not need...that mutation be selected/screened? A4: Before starting your experiment, consider co-transfecting with... cells (for example, plasmid with a puromycin resistance cassette, such as PX459). After you co-transfect...

...the plasmid here yet? A quick look at your order status on addgene.org identifies the hold-up. Your Material...Golden Stopwatch Award recognizes those TTOs with outstanding material transfer agreement (MTA) turnaround ...request MTAs and deposit agreements to basically instantaneous in many cases.  Suggest that they get an Addgene...possible new awardees, and we’re happy to share stats and strategies with tech transfer offices. Drop ...Sloan-Kettering Cancer Center  Monash University  Montana State University  National Institute of Diabetes & Digestive...

...and all the shortcomings and mistakes pointed out. Therefore, I try to stay away from the loaded and judgemental...ensuring that published work meets the accepted standards of reporting in the discipline and reduces the...underscore the sentences that I found hard to understand or individual words that are not appropriate ...whoever cares to listen. In March 2020 she will start her own research group at Boyce Tompson Institute...

...use, and likes to start at the beginning, shepherding a new product from the idea stage through all of its... days or even weeks. Primer design was done painstakingly by hand. Records of plasmid construction were... plasmids contain features such as antibiotic resistance markers and replication origins, but there is...efforts, such an algorithm has limitations. For instance, it could miss a common feature if the sequence...

...antibiotic/herbicide resistance and fluorescent proteins. Selection using resistance genes usually involves... editing, transformation may be identified by resistance to an antibiotic. Successful editing of transformants...requiring the destruction of plants or tissue for GUS staining or are not suited to studying large samples. RUBY...may contribute to the emergence of antibiotic resistant disease. (RUBY-red: You can’t beet it!) About...

... and communication. A great approach to fully understand and acquire competitive skill sets in a myriad...with a strong impact, or a journalist does not understand the high-level science discussed in an interview...your project may not advance beyond its initial stages. Help you build a network outside of the academic... incorporating EAs into your routine.  Getting started: Planning your career path and identifying needed...Science writing competitions (e.g. Access to Understanding)   Writing Communication Publicity ...

...out to start the conversation. Many researchers are open to sharing knowledge and establishing collaborations...perform research there temporarily. The length of stay could be from a month to a year. The researcher ...take good notes, and ask questions if you don’t understand something. Offer to help out wherever you can...a really good time! If it sounds right for you, start thinking outside of your current research box, send...

...indicating translational repression. During later stages, single RFP-labeled osk and Oskar protein were ...mRNA and keeps it in a single field of view for constant tracking. You can then directly monitor multiple...used harringtonine, a translation inhibitor that stalls ribosomes after initiation, to calculate a ribosome...silent. Both of these methods increase our understanding of translation dynamics, but they come with ...translation in various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide...

...profound, and this is a rare instance in which "profound" seems like an understatement. Multiple speakers from...be of secondary importance (or tertiary, or non-existant). On the very first night of the iPSC symposium... Christine Mummery, Lorenz Studer, and Deepak Srivastava--addressed a battery of questions regarding topics...implant human kidney progenitors into a pig. In stark contrast to recent genome engineering technologies...

...individual fluorescent protein (brightness, photostability, and so on; see the previous blog post for ...CFP and GFP (ex 488 nm / em 507 nm) show some crosstalk between the two fluorescent proteins. If you must... microscope. Ideally, you would like to have substantial overlap between the excitation and emission filters...most recently published ones), they can be a good starting point. In many cases it is sufficient to use a...screenshot from the Chroma Spectra Viewer comparing a standard Cy3 or Rhodamine filter set (Chroma #49004) to...

... and your career ambitions. First, how does one stand out amongst a pool of successful applicants and ...the end of your PhD or contract, you should be starting to send out polite emails with your CV to people...with others to accomplish your goals. You must understand your own style before making this choice.  How...You won't always be in the zone, but even brief stays there can make all the difference. Choose a lab ...Achieve Success in Academia  Advice for Scientists Starting a Lab   ...

... includes biochemical properties, previously established uses, and intended applications. Within CasPEDIA... different enzymes. CasPEDIA is meant to be a constantly updating database which serves as a resource ...That’s a lot of helpful information! To get you started, we’ll give a quick overview of each CasPEDIA feature...activity. AsCas12a, on the other hand, generates staggered DNA nicks at its target in cis and cleaves ssDNA...the previous information may be all you need to start your targeting experiment. However, many situations...

...Jiang,  et al. Cytokine/Jak/Stat signaling mediates regeneration and homeostasis in the Drosophila midgut...geneticists to study gene function. In this final installment, I’ll provide a brief introduction as to how ...flies? Sure, the vector will have an antibiotic resistance gene to select for transformed bacteria, but ...crosses with balancer chromosomes to make genetically stable fly lines. After a few weeks of waiting, your flies...

...function and a well established cellular signaling gene, thus providing a starting point for downstream...per gene approach that has been the historical standard. Specifically, in our recent publications, we ...profiling, systematic tumorigenesis assays, drug epistasis assays, and morphological profiling can be used...essence in clinical settings, looking ahead, we’re starting to build saturating libraries of all possible ...

...Next-generation sequencing technologies are the gold standard but they remain cost-prohibitive for many labs...Since there are many thousands of partitions, statistical modelling can be used to determine the actual... measure of target abundance, does not require standard curves, and can theoretically detect a single ...reference probe designed to bind at an unedited site distal tothe predicted double strand break, and a NHEJ...individual target molecules based on their mutation status. The drop-off probe is designed to perfectly complement...

...In the middle of 2016 Addgene started distributing a small but growing catalog of ready-made AAV and ...represents Addgene’s largest new initiative since we started distributing plasmids in 2004. We’ve already distributed... 501(c)3 nonprofit and our plasmid service was started based on donations. That seed money was used to...Wyss Institute were instrumental in helping us establish consistent, high-quality protocols for lentiviral... allows us to maintain Addgene’s high quality standards?” Under the leadership of Addgene’s co-founder...

...and joined each fragment with one part of an established pair of proteins (iLID and SspB) that undergo...population, or identify differentiation and disease states. This collection of reporter plasmids is amenable...Weinstein  Listen to the CRISPR podcast segment Understanding the connection between specific genes and an...an immune response has been a longstanding challenge. Using shRNA, TALEN, or ZFN to edit immune cells ...non-immunogenic violet-excited GFP variant Vex. To understand the impact of this gene editing tool on the immune...

...Department of Stem Cell and Regenerative Biology is to understand the basis for cardiovascular and metabolic human...question – cardiomyocytes, adipocytes or neurons, for instance – to see how those mutations influence the disease...are the implications of this kit in terms of understanding human disease? Musunuru: The power here is that...which seem to contribute to a particular disease state. You could use the kit to insert all of those variants..., CRISPR may be more efficient. You might often start with CRISPR/Cas9, but TALENs do have more flexibility...