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  2. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...edits but the double strand breaks it introduces makes it difficult to perform two or more integrations...targeting another site of interest can be used to make other edits. Once you have all the edits you want...

  3. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo...S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b...

  6. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...standard curve of known concentration. We regularly make and validate fresh plasmid standards. We validate...determine accuracy and reliability. When doing this, we make a judgement call on whether the two titer values...

  7. Hot Plasmids - November 2023

    Type
    Blog Post
    ...effective and with no additional off-target edits, making them ideal for size-limited AAV delivery. PE6c ...and MMLV gag, to specific cell types. It can also make CAR-T cell therapy more accessible by simplifying...

  8. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...buffer components can interfere with conjugates. Make sure to do your research before conjugating at the...The assay only uses one antigen binding antibody, making it less specific than other methods. In addition...

  10. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...for whom English is a second or third language (making publishing their research all the more challenging...Washington in St. Louis, offer guidance on how to make a low-cost whiteboard video about your research....

  12. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...microscopy techniques. FRET measurement sensitivity makes it suitable for studying interactions within living...approaches and appropriate controls are required to make this method useful for dynamic experiments in which...

  13. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...replication are necessary to replicate plasmids and make sure the cells maintains it. Here, we'll cover the...on type of RNA transcript, you will also need to make sure your plasmid has a promoter suited to working...

  14. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...contributed to this post. Adeno-associated viruses (AAVs) make fantastic gene delivery vehicles for episomal gene...approach requires G418 selection of infected clones, making it unsuitable for most in vivo applications.  Third...

  16. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... available as lentiviral plasmids if you want to make a stable cell line. This design allows highly localized...bioRxiv, under a CC-BY-NC-ND 4.0 license.   Just make sure you don’t overdo it! The moderately-enhanced...

  17. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...minimizes sample loss while also supporting imaging, making it easy to identify dozens or hundreds of particles...filament expressed in astrocytes throughout the brain, making it a popular target for labeling these cells. Check...

  18. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...express multiple integrins that bind the same ligand, making it difficult to dissect the function of one integrin...protein research institute, partnered with Addgene to make our collection of integrin antibodies available ...

  20. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...easy, but the risk of PCR-introduced mutations can make alternative approaches more favorable if you want...restriction sites. Alternatively, if you want to make your protein non-functional, you may simply introduce...
Showing: 541 - 560 of 795 results