...Golden Gate Modular Cloning Toolbox for Plants. Carola Engler, Mark Youles, Ramona Gruetzner, Tim-Martin...

...many genes simultaneously. These parts have been carefully engineered to support reliable functional composition...

...the second stop and carefully raise the pipette straight out of the buffer. Carefully load your samples ...occasionally as the solution heats up.). Caution HOT! Be careful stirring, eruptive boiling can occur. Pro-Tip It...not added EtBr to the gel in the first place. Carefully load a molecular weight ladder into the first ...the electrodes from the power source, and then carefully remove the gel from the gel box. Optional: If ...

...with a reporter enzyme. For multicolor staining, careful consideration must be used when selecting which...

...how will you use these systems in your research? Cary Valley contributed to updating this article. References...

... manual droplet generator, you will have to be careful about transferring your droplets from the droplet...

...below. The main requirement for IVA cloning is carefully designed primers. The majority of mistakes are...

...2007-02-062117. PMID: 17510320. Tollefson, A. E., Scaria, A., Hermiston, T. W., Ryerse, J. S., Wold, L. ...

...distortions to a minimum with protocol optimization and careful execution, but they cannot be entirely eliminated...

...hours, so read up on the timing of this approach carefully (Lin et al., eLife)! Use a Cas that facilitates...

...science and society." Science 376, (2022): 250-252. Carlson J, Henn BM, Al-Hindi DR, Ramachandran S. Counter...

...Mozziconacci O, Schöneich C, Freund E, Ricci MS, Carpenter JF, Lengsfeld CS. Do not drop: mechanical shock...

... the outcome of your IF experiment and deserve careful thought and attention.   * There are other methods...

...Its main limitation is the small size of its DNA cargo - 4.8 kb at most - which means that a gene larger...

...experiments must be set up, conducted and analysed with care to get reliable information. Although two-hybrid...

... Caroline LaManna...

...line. When expressing a viral construct transiently care needs to be taken to ensure that the proper cell...

...up with new colors (Fig. 3), such as Radish and Carrot, and further means to increase aptamer stability...

...the themes in the field and to learn what people cared about. I also used reviews to get a list of research...

... clones. The entry clone and destination vector carry different antibiotic resistance markers (indicated...