...sometimes called “VUS”) function is unknown. How can we make a decision for each patient if the majority of information...high-throughput manner and therefore push us closer to making cancer precision medicine a reality. Challenges...have worked closely with our Addgene colleagues to make these available to the scientific community. Functional... Institute of Harvard and MIT and is focused on making precision functional genomics a reality for future...

...Zetsche, B., Gootenberg, J., Abudayyeh, O., Slaymaker, I., Makarova, K., Essletzbichler, P.,  Zhang, F. (2015... diagram below for an example of how this works. Make sure to order 5’ phosphorylated oligos or treat ...single plasmids are generally more efficient. Pro-Tip Make sure to reverse the orientation of the crRNA’s direct...

...feature, you can compare different Cas enzymes to make sure you are getting the desired features and cutting...and discover orthologs with similar properties (Makarova et al). CasPEDIA is an evolving database, and... to it! The CasPEDIA Consortium will, of course, make efforts to keep it updated with new entries and ...Structural Biology. https://doi.org/10.32942/X2C31F     Makarova, K. S., Wolf, Y. I., Iranzo, J., et al. (2019)...

...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...Parton Lab recently created a flexible system that makes use of pre-existing transgenic lines, termed BLITZ...system provides researchers better flexibility and makes the approach more accessible, as it reduces the ... be packaged into an AAV system. dCas9-SSAP will make the editing of kilobase-scaled sequences more efficient...

...will be a positive asset throughout your career? 1. Make scicomm practice a priority The good news for young...of the work you presented may also be unclear. 2. Make the audience your primary focus When writing a paper...address that 'why' question effectively, you need to make your audience the focus of every sentence you write...know this? How will they react to it - what will it make them think? Does this lead to the conclusions I ...

... task will keep you from repeating your work or making similar mistakes in the future. Stay organized...camelCaseFormat since spaces between each word can make it difficult to accurately call a file. Version...your exact changes. Alternatively, you can simply make a note in your methods section about the edit that... Your Experiments Try out the CRISPR Software Matchmaker Additional Resources on Addgene.org Find Protocols...

...behind. While commercial kits are available for making small point mutations, large deletions/insertions...Current SDM methods rely on in vitro assembly. To make a point mutation, you design two oligonucleotide...instead of relying on in vitro assembly, they could make the E. coli do the work by using the principles ...-12 kb range. REPLACR-mutagenesis also requires making your own electrocompetent cells in which the phage...

...between blue and white colonies. Take care in making your plates: X-gal is light and temperature sensitive...autoclaving. If spread on top of pre-made plates, make sure it is evenly distributed and allow sufficient...specific analysis, like PCR or restriction digest. Make sure you use a proper E. coli strain (i.e. contains...JM109, STBL4, JM110, and Top10 are a few examples. Make sure you use a proper plasmid (i.e. contains the...

...how the new kit works, the research the kit now makes possible, and how it compares to his CRISPR/Cas9...step. The kit also has other unique aspects that make it especially well-suited for use in stem cells....hands of pretty much all investigators. Our kit makes it extremely simple to design TALENs. It only takes...apples-to-apples comparison of all those mutations. If you make induced pluripotent stem (iPS) cells from patients...

... a little thoughtful planning, affinity tags can make protein purification a cinch. Types of affinity ...that is unlikely to perturb protein function. This makes it amenable to being left on your protein of interest...

...a means to gain employment, so it’s important to make sure a masters helps you get a job you want. But...bench. So if you enjoy wielding a pipette, it might make sense to earn a master’s instead of a PhD. Deciding...

...in large supplementary data files, BioGRID ORCS makes it possible for researchers to query all public ...conditions. This increased ease of access not only makes CRISPR screen results available to the entire biomedical...

...temperatures and unstable ground around these hot springs make them extremely dangerous, we used telescoping poles...protocol. IgnaviCas9’s incredible thermostability makes the enzyme particularly useful. Additional resources...

... with short generation times and high fecundity, make zebrafish ideal for high throughput studies of optogenetics...depends on these charged particles and whether they make the inside of the neuron more positive or more negative...

...in healthy patients. However, the R206H mutation makes ACVR1 responsive to members of the ubiquitous activin...awareness of FOP. Please consider running with us or making a donation via the link below. Please continue ...

...scientists commonly rely on RNA isolation kits to make their life easier. Recently, we published a blog...cold and work quickly to mitigate RNA degradation. Make sure to use DEPC-treated or RNAse free water. If..., 2006).  View our protocol for RNA extraction! Making the simple protocol even more foolproof (still ... if you need really clean RNA, it’s important to make sure that when extracting, to only take the aqueous...

...properties of the fluorophores you are considering to make sure that they’ll be compatible with your instruments...properties allow for efficient signal amplification, making biotin conjugated antibodies good options for detecting...antibody. 2) Endogenous cysteines, such as those that make up the disulfide bonds between antibody chains. ...antibodies yourself, there are kits available to make the process quick and easy. Before getting started...

...experiments, and here we will focus on a unique gene that makes the popular GatewayTM method possible: ccdB. But... more about it? Read on to find out how ccdB can make your cloning experiments a little easier. One of...contain your insert of interest. Simply stated, ccdB makes cloning easier by selecting against vectors that...not contain the recombinant plasmid and therefore makes the cloning process much more efficient, as one ...

... see who you are when you look at their profile. Make your professional contact information available.... be important for a good friend, but if you are making a professional inquiry, you always want to read...type of connection can help you gather information, make introductions, and give advice for your interviews...should be as good or better than your resume Don’t make the mistake of having a shoddy profile and not updating...

...address the research challenge. Now is the time to make bold statements. While some of the statements may...6-12 months to give feedback on the application. Make sure to invest time and consider the reviewer's ...the next application. Although some comments may make it clear that the reviewer misunderstood the point...that was being made, take it as an opportunity to make clearer statements on the next round of applications...