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  2. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...This is the role of Gag proteins: they contain the matrix, capsid, and nucleocapsid viral components needed...yield dsDNA. Unlike LTRs, ITRs are perfectly symmetrical to allow for this pairing to occur. Like LTRs...

  3. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...focus on development, along with the inherent trickiness of using and optimizing GCE, led to a large toolbox...balance of trade-offs. This balance can be extremely tricky to find: each of the factors can negatively impact...

  4. How Addgene Creates Content

    Type
    Blog Post
    ...knowledge, related to the types of materials we help distribute and the research communities that we serve. ...on a case-by-case basis, as many of our blog contributors primarily write in the areas in which they have...

  5. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...References and resources References Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson ..., Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P, Frenzel A...

  7. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...This blog post was contributed by guest blogger Kate Palozola Traditional lab notebooks just won't cut... best way to find a system that does is through trial and error, and by asking for tips from others. Comment...

  8. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...top of pre-made plates, make sure it is evenly distributed and allow sufficient drying time before use. ...candidates for more specific analysis, like PCR or restriction digest. Make sure you use a proper E. coli ...

  9. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...Last updated Sept 1, 2020. This post was contributed by David Wyatt and Dale Ramsden, UNC at Chapel Hill...mutations at off-target sites. Christina Mork contributed to the update of this post. Thank you to our ...

  10. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...This post was contributed by Nikolai Braun and Keira Havens, co-founders of Revolution Bioengineering... science, these tools sometimes have limited distribution. We hope that will change with Addgene’s expansion...

  11. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...library in the appropriate combination and do a restriction enzyme digest to cut out the inserts followed...gene or in multiple genes, all of which seem to contribute to a particular disease state. You could use ...

  12. dTAG - You're it!

    Type
    Blog Post
    ...This post was contributed by guest blogger Behnam Nabet, a postdoctoral fellow at Dana-Farber Cancer ...of endogenous proteins (notably, BRD2/3/4, CDK9, TRIM24, FLT3, BTK, and ALK) by linking small molecules...

  13. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...antibody and a ≥100-fold increase in signal over intrinsic fluorescence. Increased signal is likely due to...stained with two HRP RANbodies if the sample is stripped. HRP-RANbodies are, however, compatible with most...

  15. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...Greek for “self-eating”) is induced by a lack of nutrients and targets cytosol and organelles to the vacuole... have fused with the vacuole or a lysosome are trickier to identify because their contents can be at various...

  17. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...approaching it. These include the traditional restriction enzyme/ligase-mediated method, the more recently.../19 vectors and demonstrating the concept of restriction enzyme-mediated disruption of ccdB, leading to...

  18. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...channels that are responsible for maintaining the nutrient and ionic balance of the cells,” says Dr Sangeetha...keratinocytes, which release inflammatory mediators that trigger the activation of dendritic cells, macrophages,...

  19. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...These modifications result in conformationally restricted RNA which improves mismatch discrimination and... specifically ones which do not contain a 5’ triphosphate in the gRNA, reduce the innate immune response...

  20. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...Differences in sample processing, fixation, antigen-retrieval, and permeabilization will influence how the antibody...support team, is a safer bet than one from a large distributor that may not have direct experience with the ...
Showing: 521 - 540 of 882 results