...end-joining, but will also efficiently ligate many undesirable structures, including substrates containing gaps...fidelity") better than T4 DNA Ligase (21, 25, 26). Despite this higher fidelity, Taq DNA Ligase can still ...possible NN pairs in the template. The probes were designed such that each possible pairing resulted in a ... high fidelity ligase, along with careful probe design and reaction condition optimization, will be critical...

...to be present and recognizable by your antibody despite the manipulation. Additionally, not all sample ...large deletion that removes the entire exon that codes for the epitope recognized by your antibody. You...specific, then you expect that the bulk of the peptides identified to be from the target protein. This...tuned for ready-to-use antibodies from Addgene Designing a recombinant validation experiment? Find plasmids...

...response element (RRE), an internal promoter, and the desired gene (transfer vector plasmid), 2) the HIV-1 genome...process is to test several protocols that have been described (protocol 1, protocol 2, protocol 3) to find the...quantify viral RNA. All of these methods have been described in a previous Addgene blog Once you know your ...Miller, A. DUSTY, and C. A. R. O. L. Buttimore. "Redesign of retrovirus packaging cell lines to avoid recombination...

...update, we have added new sections to share and describe some incredible new CRISPR technologies developed...much-anticipated prime editing section and additions describing CRISPR’s ability to make large genome edits using...transposases or the Cascade-Cas3 system. We also describe newly engineered Cas9s with more flexible requirements...

...eBooks, the Antibodies 101 eBook is designed to be a helpful desktop reference. Each chapter brings together...an Antibody” quite useful. If you’re looking to design your first flow cytometry experiment, “Chapter ...

...When performed correctly, fluorescence microscopy provides a high contrast image in which a bright signal...specificity to the viewer. Finally, modern microscope designs may further exploit the unique characteristics ...one object in the final image. Over the past two decades a number of techniques have been developed that...resolution and decreased phototoxicity. Lightsheet designs that allow for multi-view imaging can image thick...

...been infected with a microbe, to trigger immune cascades that clear these infections. Anything that generates...immune response and initiates different immune cascades. In the research setting, antibodies of different...antibody, which recognizes the primary antibody and provides the method of detection. You’ll also want to use...used for. Addgene’s Antibody Plasmid Collection includes plasmids that express antibodies, nanobodies, ...

...occurring earlier but that the photo evidence was destroyed to protect the innocent revelers. Our Halloween...for Halloween and how seriously we take costume design. In case you need more convincing, I’ve got just...to great customer support by dressing up as our Zendesk email system.  Our 2013 winners dressed up as ...

...single gene KO and pooled screening approaches; (4) encodes a Thy1.1 selectable marker to facilitate the isolation...assess T cell responses and clonal dynamics in lymph nodes (LN) and tumor cells. Figure used with permission...also reveal novel pathways. The FITS deposit also includes pLX_305_Ovalbumin, a plasmid that enables introduction...

...2022, from the launch of a new material to the redesign of a community resource. Now that we’re in the...antibodies. Everything in this collection, which includes both neuroscience and general-use antibodies, ...website saw 2.2 million visitors from 231 countries. Guides and protocols were our most popular content, while...

...and the Addgene Help Center. A big part of this redesign was pulling all of our science options into a ...Addgene’s website easier to navigate, thanks to design choices informed by Web Content Accessibility Guidelines...word for it — check out all the new features and designs addgene.org! And when you’re finished, feel free...

...Feng Zhang’s lab at the Broad Institute and was designed and cloned by Neville Sanjana. This fantastic ...your gRNA of interest to mammalian cells. It was designed to have increased lentiviral titer as compared...sampling of the research it has already been used for includes work probing mTOR signaling in cancer cells, deciphering...

...one-day conference aimed at the general public that provides an accessible introduction to various scientific...science cafes, and Reddit AMAs. Their website also provides a variety of short- and long-form written content... information about SITN: DayCon event website (includes archived content) Science in the News website ...

...part of the story. This year, we made significant strides in making science more accessible and reliable....supporting the tools in our collection, such as blogs describing viral vector serotypes, or hundreds of Data Hub...detailing antibody experimental results. We also redesigned our website to make it easier to find what you...

...of familiar flowers." This new x-ray technique provides scientist with a new method to explore the inner...of delicate plants and other organisms without destroying the original, which could provide a new tool ... collected by Darwin on his voyage. Previous destructive techniques were incompatible with the need to...

...process When I first saw the comprehensive job description for this co-op, I was immediately interested ...had stood out to me because it gave me a full description of the tasks I would be responsible for, in comparison...it relates to other teams at the company. This provides new (and old) employees with information on how...

...spatial biology enthusiasts. This dataset currently includes 1049 community-validated reagents from 20 contributors... And the dataset is rich. Experimental data includes reagent type, target species, target name, host... inspired by the open-software community when designing the IBEX Knowledge-Base, seeking to create an ...

...happening inside cells for at least a couple of decades. Green is the classic color, but fluorescent proteins... greater than any fluorescent protein earlier described. “Until our proteins, there were lots of fluorescent...illumination with far-red light. As the researchers described in Nature Communications last year, “The capability...

...ve just discovered that your favorite gene was described in a CRISPR screen publication. You see this mentioned...This format is inherently flexible and allows the broadest possible scope of screens to be curated, for example...other than cell viability. The following video provides an in-depth walk through of how to use ORCS:   ...

...the region of the brain where DA quantitation is desired (Figure 1). The probe perfuses liquid, which causes...et al., 2018). These sensors also exhibit high magnitudes of fluorescent responses. Studies on the kinetics...Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors." Science 360.6396...