...Paquet, D., Teo, S. et al. Precise and efficient scarless genome editing in stem cells using CORRECT. Nat...

... - they can hold up to 8 kb, while AAV can only carry around 4.5 kb. Different AAV serotypes have preferences...

...containing structural genes and a transfer plasmid carrying the transfer gene. By dividing the viral elements...

...improved transcript life, and so should be handled with care (4). For this reason, viral vectors often employ...

... 4 °C prior to use. Thawed aliquots should be discarded after 1–2 months. 1 mg/mL polyethylenimine, linear...stored at 4 °C for up to 2 months. After 2 months, discard the tube and thaw a new working stock. Considerations...DNA tube. Incubate the mixture 15–20 min at RT. Carefully transfer the transfection mix to your HEK293T ...cells. Add the transfection mix dropwise, being careful not to dislodge the cells. Incubate the cells for...the following morning. The following morning, carefully aspirate the media. Replace the media with 15 ...

...move your microscope around the lab, be sure to carry it with two hands - one hand supporting the base...base and the other holding the arm - or use a cart. Revolve the nosepiece so that the lowest power objective...objective. Take your eyes away from the eyepiece and carefully revolve the nosepiece so that the next objective...will notice that the objectives get larger. Be careful to avoid hitting your sample with the objective...laboratory instrument, you should be sure to take care of your microscope. Bumps, scratches, and dust can...

...interest. They created a selection phage (SP) to carry catalytically dead SpCas9 (dCas9) fused to the bacterial...

...alter for a purpose and To direct the professional career of. Like everyone else I mostly learned through...

...now have another model of Cas9 – a flexible alpha-carbon backbone model made of nylon – that more accurately...

...your mutation. To maintain stable lines of flies carrying a specific mutation, Drosophila geneticists use...

...PMCID: PMC3074624. 5. Bindels, Daphne S., et al. "mScarlet: a bright monomeric red fluorescent protein for...

... the plasma membrane by fusing various vesicle cargoes to FP timer mk-GO (Tsuboi et al.) Monitor gene ...

... plasmid collection Browse the RESOLUTE solute carriers collection Find other COVID-19 and Coronavirus...

...the Biohub in San Francisco. Before starting his career in software, he completed a MSc in Psychology at...

...reviews journal. She joined Cell Press to pursue a career in science communication and publishing after completing...

...resolution of positive and negative populations, Careful panel design In both conventional and spectral ...

...achieve greater efficacy, researchers will have to carry out repeated transfections with the ssDNA guide,...

...could be forming dimers in cells. Why should you care? Three simple ways a dimeric FP could ruin your ...

...Pubmed. C. Soderblom, D.-H. Lee, A. Dawood, M. Carballosa, et al, (2015) eNeuro, March/April 2015, 2(2)...

...segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes...