...plasmids pX330S-2 to pX330S-(last gRNA number). The 5’ most gRNA is cloned into the Cas9-containing destination...Danilo, et al. “In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system.” ...

...many applications in biotech, and is considered the most studied Gram-positive bacterium, many of its genes...looking for the production of ECT-DNA (extra-chromosomal telomeric DNA), which accumulates when T-loops...

...the wild-type state, and is thus thought to be the most suitable virus for therapeutic applications. Clinical...host genome at a specific site, AAVS1 on human chromosome 19. This site is favored due to the presence ...preferred site of genomic integration on human chromosome 19, termed AAVS1. Instead, the rAAV genome is...molecular weight structures that are maintained extrachromosomally. rAAV are more likely than wild-type AAV ...been identified, with the best characterized and most commonly used being AAV2. These serotypes differ...

...There are 57 accepted human adenovirus types, and most adenoviral vectors are based on Ad5. Ad5-based vectors...AdEasy™, developed by Bert Vogelstein , is by far the most popular method for creating adenoviral vector constructs...The adenoviral backbone pAdEasy-1 is suitable for most purposes. For especially long transgenes, the use...promote bi-directional transcription Ad5-based vector Most common type of adenoviral vector pAdTrack Class ...

...screens POSITIVE screen: Apply library Apply selection Most cells die or do not pass selection (in the case ...selection) In parallel, apply library Apply selection; most cells will survive Perform NGS on the surviving ...The selective pressure must be strong enough that most of the cells die, removing their plasmids from the.... Check out our blog posts featuring some of our most popular libraries! Genome-wide Screening Using CRISPR...

...Core Promoter The core promoter region is located most proximal to the start codon and contains the RNA...factors promote the initiation of transcription. The most 3' portion (closest to the gene's start codon) of...eukaryotes and archaea, however, contain this TATA box. Most prokaryotes contain a sequence thought to be functionally...contains transcription factor binding sites, but mostly contains regulatory elements. Eukaryotic Promoters...

...of viral integration have shown that lentiviruses most often integrate into actively transcribed genes,...p75 binding sites are enriched in gene bodies and mostly absent in promoters and intergenic regions, mirroring...promoter within the transfer plasmid must be modified. Most shRNA‐expressing lentiviral plasmids, such as pLKO...Broad tropism envelope protein used to psuedotype most lentiviral vectors. Packaging Gag in trans Precursor...

... pathways targeted to specific cell populations (most often neurons) that can be turned on or off by the...have few, if any off-target effects. They are the most widely employed type of chemogenetic receptors. ...: hM1Dq, hM3Dq, and hM5Dq, however, hM3Dq is the most widely employed. Gi-DREADDs signal through the Gαi...

..., Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses, lentiviruses...

...Addgene is poised to reach in the near future! We’re almost at:  100,000 different plasmids available from...

... will expand the repository in ways that will be most useful to you, the members of our community.   ...

...CRISPR guide is a comprehensive resource that covers most things CRISPR - from nickases to base editing to...

...Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  On the tenth day of CRISPR, Addgene...

...April Fool’s Day on the Addgene blog.  We’ve been mostly working remotely for the last year to give our ...

...YanevCellosaurus: A Cell Line Information DatabaseResearcher: Amos BairochViral Vectors 101: Systemic CapsidsResearchers...

...by Maki Naro   3)   “Bird and Moon” by Rosemary Mosco   4)   “Unearthed Comics” by Sara Zimmerman   5)...

...conference blog posts before you go!  Tips to Make the Most of a Scientific Conference Poster Session TipsHow...

... it regularly as people send me suggestions. The most useful parts of the Guide are probably the papers...

...GIPHY   We’ve been working hard to create the most informative and entertaining protocol and explainer...

...breakdown Introduction to Vini, Amy, and SITN The Most Difficult thing Vini, Amy, and Outreach Scientist...