We narrowed to 599 results for: arl

1. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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   Blog Post

   ...PMCID: PMC3074624. 5. Bindels, Daphne S., et al. "mScarlet: a bright monomeric red fluorescent protein for...

2. The Scientific Conference Poster Session: Tips for Success

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   Blog Post

   ...business cards to tack up next to your poster. Arrive early: You might be nervous before your poster session...

3. Plasmids 101: Optimizing Plasmid Yields

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   ... might need to use multiple techniques for a particularly stubborn prep. Check the bacterial strain Increase...

4. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   ...increasing the signal from the second FP. Several early FRET biosensors were made with CFP and YFP, cyan...

5. Experimenting with New Careers while in Grad School

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   Blog Post

   ...Indiana University School of Medicine. She is particularly interested in science communication and fundamental...

6. PITChing MMEJ as an Alternative Route for Gene Editing

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   Blog Post

   ... TALENs and CRISPR. MMEJ is active during M and early S phases, when HDR is inactive, and the balance ...

7. Genome-wide Screening Using CRISPR

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   Blog Post

   ...cell types that are of low abundance are not particularly well suited for genome-wide screening. Avoid...

8. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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   ...www.protocol-online.org/ Protocol Online is one of the early protocol sharing websites. It was created in 1999...

9. Plasmids 101: Multicistronic Vectors

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   Blog Post

   ...the same cassette is sometimes advantageous, particularly when only a portion of the plasmid is packaged...

10. Tips for Using BLAST to Verify Plasmids

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    Blog Post

    ...COBALT for aligning multiple protein sequences, particularly for comparing different isoforms. In addition...

11. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    Blog Post

    ...many efforts in open science, Endy was recognized earlier this year by the White House as a Champion of Change...

12. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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    Blog Post

    ...reagents, making it more difficult to detect. A particularly strong absorber at 230 nm is guanidine thiocyanate...

13. Great Results Start with Great Standard Curves

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    Blog Post

    ...consistent dilution factors, change pipette tips regularly, and mix samples very well.  Use duplicates or...

14. Antibodies 101: Producing Recombinant Antibodies

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    Blog Post

    ..., Zhan C, Malm M, Field R, Turner R, Sellick C, Varley P, Rockberg J, Chotteau V. Small-scale bioreactor...

15. Getting to Know Expansion Microscopy

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    ...the protocol, samples can be expanded to ~4-10x linearly! This expansion physically separates molecules...

16. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    ...adventure, just remember that even though these early steps don’t even involve antibodies, they are still...

17. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post

    .... Most notably, China was affected by COVID-19 earlier than the rest of the world, from mid-January to...

18. DNA Purification Without a Kit

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    Blog Post

    ...extraction method described in Kim and Morrison is nearly identical to that of commercially available kits...

19. Droplet Digital PCR for AAV Quantitation

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    Blog Post

    ...droplet separation (Witte et al., 2016). This is particularly important if your amplicon is longer than 150...

20. MXS Chaining

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    Blog Post

    ...robustly, quickly, and simply. We hope that you can similarly apply MXS chaining to your experimental needs....