We narrowed to 593 results for: arl

1. PITChing MMEJ as an Alternative Route for Gene Editing

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   Blog Post

   ... TALENs and CRISPR. MMEJ is active during M and early S phases, when HDR is inactive, and the balance ...

2. Genome-wide Screening Using CRISPR

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   Blog Post

   ...cell types that are of low abundance are not particularly well suited for genome-wide screening. Avoid...

3. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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   Blog Post

   ...www.protocol-online.org/ Protocol Online is one of the early protocol sharing websites. It was created in 1999...

4. Plasmids 101: Multicistronic Vectors

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   Blog Post

   ...the same cassette is sometimes advantageous, particularly when only a portion of the plasmid is packaged...

5. Tips for Using BLAST to Verify Plasmids

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   Blog Post

   ...COBALT for aligning multiple protein sequences, particularly for comparing different isoforms. In addition...

6. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   Blog Post

   ...many efforts in open science, Endy was recognized earlier this year by the White House as a Champion of Change...

7. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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   Blog Post

   ...reagents, making it more difficult to detect. A particularly strong absorber at 230 nm is guanidine thiocyanate...

8. Great Results Start with Great Standard Curves

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   Blog Post

   ...consistent dilution factors, change pipette tips regularly, and mix samples very well.  Use duplicates or...

9. Antibodies 101: Producing Recombinant Antibodies

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   Blog Post

   ..., Zhan C, Malm M, Field R, Turner R, Sellick C, Varley P, Rockberg J, Chotteau V. Small-scale bioreactor...

10. Getting to Know Expansion Microscopy

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    Blog Post

    ...the protocol, samples can be expanded to ~4-10x linearly! This expansion physically separates molecules...

11. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post

    .... Most notably, China was affected by COVID-19 earlier than the rest of the world, from mid-January to...

12. DNA Purification Without a Kit

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    Blog Post

    ...extraction method described in Kim and Morrison is nearly identical to that of commercially available kits...

13. Droplet Digital PCR for AAV Quantitation

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    Blog Post

    ...droplet separation (Witte et al., 2016). This is particularly important if your amplicon is longer than 150...

14. MXS Chaining

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    Blog Post

    ...robustly, quickly, and simply. We hope that you can similarly apply MXS chaining to your experimental needs....

15. Tips for arabidopsis transformation

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    Blog Post

    ...transformant. Use young plants: If you transform early, your plants will still have plenty of time to produce...

16. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    Blog Post

    ...transfection (B in the figure above), are in the early stages of development. David Liu’s Lab has demonstrated...

17. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    Blog Post

    ...journal articles. From paper to plasmid Authors regularly indicate that their plasmids are available from...

18. AAVs CREATed for Gene Delivery to the CNS and PNS

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    Blog Post

    ...difficult it is to trace individual cell’s axons, particularly the two green neurons near the arrows. When ...

19. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    Blog Post

    ...adventure, just remember that even though these early steps don’t even involve antibodies, they are still...

20. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post

    ...cargos must be flanked by the L and R ends - thus, scarless insertions are not currently possible, unlike ...