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  2. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...applications, SEC selection could replace other, more restrictive C. elegans selectable marker systems. This novel... the Caenorhabditis elegans genome using Cas9-triggered homologous recombination. Dickinson DJ, Ward JD...

  3. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ..., there is always another secret."     - Marcy Patrick, Associate Director of Quality Control Jonathan...from the Ice Age to the 1980s, Sarum follows an intriguing cast of characters from five different families...

  6. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ... This guest post was contributed by Marco Straccia, an Associate Professor at University of Barcelona...governed how this game was played. These rules restricted which cards could be played when and, depending...

  7. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...help make this easier for scientists, we now offer trial size 20 µL samples of control AAV that scientists...mCherry 50467  AAV2 pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine 121538  AAV5 pOTTC1484 - pAAV SYN1 HA-hM4D(Gi...

  10. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...(by CRISPRainbow) to 3-fold with CRISPR-Sirius. Triple color detection With a third sgRNA containing alternating... U2OS cells.   Figure 2: CRISPR-Sirius tricolor labeling. (A) Diagram of sgRNA-Sirius-8XMS2, sgRNA-Sirius...

  11. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated glass to...workshop. Future workshops for Addgene Addgene strives to be a great resource for the research community...

  12. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...This post was contributed by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela ...other barriers (like vasculature or extracellular matrices) in response to pH changes. Beyond these applications...

  13. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...This post was contributed by guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’...catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA." Nature biotechnology (2016). PubMed...

  14. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...Cofactors Secreted Luminous shrimp(Oplophorus gracilirostris) 455 19 Coelenterazine None Yes Luminous ...Gaussia princeps) 480 20 Coelenterazine None Yes Metridia longa 480 24 Coelenterazine None Yes North American...

  15. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...tissue types, recombinases allow you to further restrict gene expression to areas in which the recombinase...expression patterns, thus allowing you to further restrict gene activation to cells expressing both Cre and...

  17. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...we wanted to take a look back at some of the contributions from our depositors. Below are the 15 top requested...Zhang lab codon-optimized SpCas9. Using the BbsI restriction site, a pair of annealed oligos can be cloned...

  18. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...References and resources References Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson ..., Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P, Frenzel A...

  19. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...This guest post was contributed by Nathan Sanders of ComSciCon, the Communicating Science Conference ...employer that your past work has prepared you to contribute at a high level to their team.  When you're writing...

  20. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...Chromatin Cheryl Arrowsmith James Bradner Kerstin Bystricky Courtney Hodges Sharon Dent Archa Fox Elaine...theReddyLab), Thoru Pederson, Joanna Wysocka, Kerstin Bystricky, Ben Martin, and Berkley Gryder. Addgene depositors...
Showing: 501 - 520 of 882 results