We narrowed to 772 results for: Dos

1. Addgene at the ASM Microbe 2016 Conference

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   Blog Post

   ... the fantastic work this Broad Institute lab is doing creating well-optimized CRISPR screening technology...

2. Seeing Red: Simple GFP Photoconversion

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   ... and GFP does not display auto-photoconversion as do some FPs. "While this discovery is likely to be most...

3. Future of Research Conference - Remarkable Opening Session

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   ...for change in their opportunities.  Scientists must do science to be happy so the beginnings of solutions...

4. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

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   ...could, and Addgene is a wonderful vehicle for us to do that.” At Addgene, we are equally excited to be helping...

5. The Stingy Scientist: How the Baby Gel Box Was Born

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   Blog Post

   ...mindful of the lab materials I was using.  I started to do little things to save reagents and money.  This was...

6. In Living Color: The Skinny on In Vivo Imaging Tools

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   ...Transparency window That’s exactly what they reported doing in a paper that appeared in Nature Biotechnology...

7. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

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   ... lentiviral CRISPR library.  “This enables you to do customized genetic modification on a scale that was...

8. Tag Your Favorite Yeast Genes with Ease

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   ...tagging systems enabled you to advance your research? Do you have a favorite tagging system that isn't mentioned...

9. Antibodies 101: Antibody Engineering and Directed Evolution

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   ...therapeutics. What is antibody engineering (and why do we like it)? Antibody engineering is the modification...ultimately increasing treatment effectiveness. How do you engineer an antibody? There are many, many ways...

10. Custom CRISPR Screens & the Green Listed Software

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    .... Luckily, the Green Listed software can help you do just this, probably in less than a minute (1). Green...would be the best gRNAs. Interestingly, the libraries do not overlap much, showing that we are far from consensus...

11. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    ...recombination that can lead to leaky expression in cells that do not express Cre or Flp. Here at Addgene we quantify... cells that don’t express Cre or Flp, what can we do about it?  Test the serotype and promoter The first...

12. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    Blog Post

    ...response to visible light (Zhang et al., 2011). What do microbial rhodopsins and neurons have in common? ...switch (aka DIO (Double-floxed Inverse Orientation) or DO (Double-floxed Orientation)) is a powerful tool that...

13. Antibodies 101: Introduction to Antibodies

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    ...epitopes that antibodies can recognize. Imagine you’re doing a word search puzzle. You’re not always going to...protein of interest from the mixture. Antibodies can do this, too! In this method, antibodies bound to microscopic...

14. A Tour of Addgene's Most Popular Pooled Libraries

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    ...CRISPR libraries As CRISPR technologies multiply, so do CRISPR pooled libraries! Addgene’s collection also...

15. Year of the Camelid: Antibody Style

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    ...of different cancers (Jin, et al. 2023).  So where do all these sdAbs come from? In the early years of ...

16. Antibodies 101: Affinity Tags

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    ...where affinity tags come in – they are peptides which do not naturally exist in most expression systems that...

17. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

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    ...both bioinformatics tools and knowledge databases. Do you have a favorite online molecular biology tools...

18. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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    ...enveloped viruses bud out, instead of lysing, but they do generally still kill the infected cell.) This is ...

19. Addgene Begins Distribution of Recombinant Antibodies

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    ...antibodies are excellent for reproducibility because they do not share hybridomas’ genomic drift or limited material...

20. RUBY-Red Siliques

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    ...., Sun, H. et al. Hortic Res. (2020).   I can do this by expressing a gene that rescues the mutations...