We narrowed to 769 results for: Dos
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TypeBlog Post...species. Some Cas proteins, like LbCas12a or AsCas12a, do not require a tracrRNA. sgRNA: “Single-guide RNAs...
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Addgene at the ASM Microbe 2016 Conference
TypeBlog Post... the fantastic work this Broad Institute lab is doing creating well-optimized CRISPR screening technology... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post...Your next cool experiment requires some AAV. Where do you start? Plasmids of course! You just need three... -
Seeing Red: Simple GFP Photoconversion
TypeBlog Post... and GFP does not display auto-photoconversion as do some FPs. "While this discovery is likely to be most... -
Future of Research Conference - Remarkable Opening Session
TypeBlog Post...for change in their opportunities. Scientists must do science to be happy so the beginnings of solutions... -
The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins
TypeBlog Post...could, and Addgene is a wonderful vehicle for us to do that.” At Addgene, we are equally excited to be helping... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog Post...mindful of the lab materials I was using. I started to do little things to save reagents and money. This was... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog Post...Transparency window That’s exactly what they reported doing in a paper that appeared in Nature Biotechnology... -
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
TypeBlog Post... lentiviral CRISPR library. “This enables you to do customized genetic modification on a scale that was... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog Post...tagging systems enabled you to advance your research? Do you have a favorite tagging system that isn't mentioned... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post...response to visible light (Zhang et al., 2011). What do microbial rhodopsins and neurons have in common? ...switch (aka DIO (Double-floxed Inverse Orientation) or DO (Double-floxed Orientation)) is a powerful tool that... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post.... Luckily, the Green Listed software can help you do just this, probably in less than a minute (1). Green...would be the best gRNAs. Interestingly, the libraries do not overlap much, showing that we are far from consensus... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post...recombination that can lead to leaky expression in cells that do not express Cre or Flp. Here at Addgene we quantify... cells that don’t express Cre or Flp, what can we do about it? Test the serotype and promoter The first... -
Antibodies 101: Introduction to Antibodies
TypeBlog Post...epitopes that antibodies can recognize. Imagine you’re doing a word search puzzle. You’re not always going to...protein of interest from the mixture. Antibodies can do this, too! In this method, antibodies bound to microscopic... -
The Golden Stopwatch Award: Recognizing Facilitators of Research
TypeBlog Post...why MTAs matter, check out another blog post: Why Do I Need an MTA Anyway?) Don't want to wait? Talk with... -
Sensing Neuronal Dopamine
TypeBlog Post...learning, decision making, and motor control. What do we understand about Dopamine? DA is produced by specific... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog Post... rhythmic movements like spontaneous swimming. To do so, the lab expressed the LiGluR opsin in different... -
The Strength of Story Telling
TypeBlog Post... crucial skill for career advancement. Scientists do and should tell stories all the time. They provide... -
Meet the New Addgene Board Members!
TypeBlog Post...high school, I knew that this is what I wanted to do,” Stegmeier says He began this journey in biochemistry... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog Post... the AAV data hub. How to share your AAV data Do you use Addgene AAV tools and want to join the ranks...methods. For example, you can provide location, titer dose (Example: 1E13 GC/mL) and subject age at time of...