We narrowed to 776 results for: Dos

...you high-quality educational resources to help you do better research. CRISPR is an incredibly fast-moving...

...species. Some Cas proteins, like LbCas12a or AsCas12a, do not require a tracrRNA. sgRNA: “Single-guide RNAs...

... the fantastic work this Broad Institute lab is doing creating well-optimized CRISPR screening technology...

... and GFP does not display auto-photoconversion as do some FPs. "While this discovery is likely to be most...

...for change in their opportunities.  Scientists must do science to be happy so the beginnings of solutions...

...could, and Addgene is a wonderful vehicle for us to do that.” At Addgene, we are equally excited to be helping...

...mindful of the lab materials I was using.  I started to do little things to save reagents and money.  This was...

...Transparency window That’s exactly what they reported doing in a paper that appeared in Nature Biotechnology...

... lentiviral CRISPR library.  “This enables you to do customized genetic modification on a scale that was...

...tagging systems enabled you to advance your research? Do you have a favorite tagging system that isn't mentioned...

...therapeutics. What is antibody engineering (and why do we like it)? Antibody engineering is the modification...ultimately increasing treatment effectiveness. How do you engineer an antibody? There are many, many ways...

...recombination that can lead to leaky expression in cells that do not express Cre or Flp. Here at Addgene we quantify... cells that don’t express Cre or Flp, what can we do about it?  Test the serotype and promoter The first...

...epitopes that antibodies can recognize. Imagine you’re doing a word search puzzle. You’re not always going to...protein of interest from the mixture. Antibodies can do this, too! In this method, antibodies bound to microscopic...

...response to visible light (Zhang et al., 2011). What do microbial rhodopsins and neurons have in common? ...switch (aka DIO (Double-floxed Inverse Orientation) or DO (Double-floxed Orientation)) is a powerful tool that...

.... Luckily, the Green Listed software can help you do just this, probably in less than a minute (1). Green...would be the best gRNAs. Interestingly, the libraries do not overlap much, showing that we are far from consensus...

...CRISPR libraries As CRISPR technologies multiply, so do CRISPR pooled libraries! Addgene’s collection also...

...antibodies are excellent for reproducibility because they do not share hybridomas’ genomic drift or limited material...

...., Sun, H. et al. Hortic Res. (2020).   I can do this by expressing a gene that rescues the mutations...

...EasyTag System is a straightforward workflow for doing gene targeting, reporter generation, controllable...

...able to detect extracellular stimuli. One way they do so is through G protein-coupled receptors (GPCRs)...