Skip to main content
Addgene
Showing: 481 - 500 of 795 results

  1. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...terminal repeats (ITRs). ITRs are the elements that make an AAV plasmid an AAV plasmid.  Within the AAV ...and disruptive mutations and truncations that can make vector propagation and sequencing a headache. How...

  2. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...tools out there to visualize genomic loci, what makes CRISPR-Sirius unique? Unlike fluorescent in situ...predecessor CRISPRainbow, CRISPR-Sirius is brighter making it suitable for observing low-copy genomic loci...

  3. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...working in our laboratory! Don’t worry though, we make sure that everyone gets proper equipment and safety...engineer yeast to do similar things. The focus is to make yeast engineering accessible to everyone but these...

  4. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...Our Plasmids 101 eBook! One of the features that makes NEBuilder HiFi so accurate (apart from the high-...restriction enzyme digests to DNA assembly. What makes it so forgiving, is the incorporation of a 3’-5’...

  5. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...published in the same journals as those that did make deposits to Addgene.  Insomuch as different journals... argument that Addgene is achieving its goal of making plasmid-based science more accessible…and accruing...

  6. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...and molecular biologists use to tinker with DNA. Making changes to a DNA sequence can help scientists identify... are a few drawbacks with these approaches that makes it challenging for users to achieve targeted diversification...

  7. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...If you’re using CRISPR to make a genome edit, how do you know if your CRISPR experiment was successful...different constructs for the reporter to work and to make the desired genome edit:  A donor plasmid that ...

  8. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...control for a specific marker in your experiment. To make an FMO sample, you take a subset of your experimental... is prepared very similarly to a FMO sample: you make a sample from a combined subset of your experimental...

  9. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...then inserted into the host's genome and is used to make more virus. The most studied (and used in the lab...also transduce dividing and non-dividing cells, making it conducive to targeting all cell types. Altogether...

  10. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ... Thus, the adjustable nature of viral libraries makes them the ideal vector for the job. Therapeutic ...immunogenicity, and cell-specific targeting features make them an ideal delivery system for human patients...

  13. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...     The Trimmer Lab has continued to work to make the sequences of monoclonal antibodies publicly ...sequences from the Trimmer lab hybridoma collection and make them publicly available. The lab has also expanded...

  16. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...testing and a lack of well-characterized genetic tools make it challenging to engineer a specific function in...construct our color changing flower we are carefully making our way through the IP landscape, using plant parts...

  19. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...systemically administered in an organism. These features make AAV ideal for human therapies and in vivo laboratory...delivery of genetic cargo to a host cell. In order to make room for a gene of interest, AAVs used in research...

  20. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...suppression of expression is faster and easier than making a knockout and is a great way to temporarily reduce...integrated (once or many times) into the genome. Make sure to choose an appropriate promoter to express...
Showing: 481 - 500 of 795 results