...PE6b was very efficient for short, unstructured templates and significantly reduced undesired edits (indels...complex edits, such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing...potential to reduce negative side effects. This platform offers a promising approach for experimental therapies...illuminated with blue light, catalyzing the biotinylation of nearby proteins. B) TransitID uses sequential... et al. (2023). Engineered allostery in light-regulated LOV-Turbo enables precise spatiotemporal control...

... to grow on plates or in liquid culture to amplify the library, followed by DNA isolation. The amplified...screening-scale transduction of cells, resulting in cell population mutated by the viral pooled library. These cells...payload is adequately represented in the cell population, you’ll need to infect many more cells than the...Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Genome-scale...

...peripheral blood mononuclear cells (PBMCs). PBMCs isolated from whole blood present many benefits for the...patients are reprogrammed less efficiently, no such correlation has been found between the age of the patients... amplification in the host cell. This enables relatively high and long-term expression of the reprogramming...using PBMCs as the source of somatic cells. This translates to only about 16 hiPSC colonies per 100 ml of...Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation. Stem Cells...

...in your favorite neuronal population and track connections to that population. Limitations of common genetically...to define those that target specific neuronal populations in the brain. The Allen Institute and the Janelia...also been important in mapping, monitoring, and manipulating neuronal circuits. Epitope tags are short antigenic...cells. They can be used for protein localization, isolation, and tracking experiments (Rizzo, 2009). With ...

...do a lot of DNA work but never get to actually manipulate the organism they study (unless if you count ...study of diverse organisms. Methods for genetic manipulation still need to be developed for each organism... currently for terrestrial frogs. Cnidarian-Dinoflagellate symbionts (Virginia Weis) Sea anemone Aiptasia...Henry) This black footed slipper snail can be manipulated by injected RNA and can express fluorescent proteins...is developing tools for pan-arthropod genetic manipulation. The ReMOT control system (Receptor-Mediated...

...Secretary of Labor Thomas Perez updated the overtime regulations of the FLSA. The exemption salary was set at ... postdocs are at each of these institutions (populations potentially from 1 to 5761, according to the ...research on these institutions, we can say with relative certainty that just over 40% of current postdocs...for academia Changes are afoot for the postdoc population, and it is unclear how this will all shake out...effects the new laws will have on the postdoc population. In the current funding climate combined with...

...strategy for increasing the size of the AAV donor template for CRISPR-mediated homology directed repairs ...acceptor of the transgene to help prevent the accumulation of such truncated proteins. Examples of use...homologous recombination approach delivers large donor templates using two sequential homology directed repairs... the gRNA target site that mediated the donor template’s integration so that the site is reconstituted...which results in the integration of a larger donor template.  Drawbacks: Transgene expression varies with...

...Having successfully navigated the trials and tribulations of the process, I’ve provided guidance and mentoring...interviews but no job offers, is a failure to clearly articulate one's talents and ideas. Some key points from...question? This should be something that anyone could relate to, that people would agree is important to understand...provide the first defined model system that we can manipulate to study pathology and therapies." This is...interview, your previous success will not necessarily translate into getting a job. Your papers and pedigree get...

...can do and, in this way, science has always been related to government and, consequently, politics. More...Inclusion of data informing policy specifically related to the following subjects: Climate change Vaccines...Healthcare Science education To make scientists relatable (17, 20). This is an effort to show the general...all you need to do is select your state. State Legislatures: To find the website to contact representatives...Lawmakers need to use a scientific approach to formulating views Pew Research Center: Public and Scientists...

...factor fusions modulate iPSC reprogramming efficiency. Listen to the segment on modulating iPSC reprogramming...Hot Plasmid 3: Regulating telomere length with TZAP. Listen to the segment on regulating telomere length...

...Being a scientist in my late 20’s, new graduates often ask me for advice on careers available to new ... felt I needed more practical lab experience to later gain a full time position in a lab. I worked on ...left me optimistic that I would find a genetics related role soon after finishing my MSc. The masters program...I had received all the training and experience relating to good manufacturing practice (GMP) and good ...and decided to compromise and applied anyway. I later found out the job was with Addgene. I had used plasmids...

..., generating new therapeutics, and sometimes formulating new biological concepts. However, rates of reproducibility... However, even simple procedures like pouring a plate or running a gel have intricacies that aren’t always...have plans to expand to other protein technologies later. Organizations such as BenchSci and FPbase are working...find information about research tools. BenchSci collates information about antibodies while FPbase curates... soon, the Center for Open Science does host a platform for pre-registration wherein researchers can submit...

...Tetracycline-Dependent Transactivator Correlates with Long-Term Doxycycline-Regulated Transgene Expression in Nonhuman...primate. Likewise, in vitro efficacy doesn’t always translate to in vivo efficacy. You’ll therefore need to ... strong early gene expression, but can become methylated over time, resulting in decreased expression ...consider. First, the titer required is inversely related to transduction efficacy: the higher the efficiency...

...at Warp Drive Bio. The post is in memoriam of his late father and pBABE’s namesake, Harold (Babe) Morgenstern...represents a lasting tribute to Dr. Morgenstern’s late father, Harold (Babe) Morgenstern. The origins of...vastly decreasing the cost of stable selection relative to the then heavily used neomycinR/G418 tandem... countless researchers - a fitting tribute to my late father. Many thanks to guest blogger Jay P. Morgenstern...

...investigator. In basic science (as opposed to translational, biomedical, or applied sciences), people conduct...everything there is to know is a strange and exciting revelation. Science in any field can generally be applied...neurodegenerative disease because they had close relatives suffering from similar diseases. Others were inspired...t know yet has helped me cultivate stronger relationships with my labmates. Sometimes what I thought was...students, women, BIPOC, and other minoritized populations. Research universities often have scholarships...

...broth and on the agar plates. Mycoplasma colonies are easily visible on the agar plates. This method is capable...detection, DNA staining, fluorescent tagging, agar plating, etc. Be wise to choose any of the techniques listed... (Uphoff and Drexler, 2011). Culturing on agar plates/broth is considered the ‘gold standard’ for detecting...prevent contamination rather than eradicating it later. Don’t be scared when mycoplasma infects your cell...

...genome-wide CRISPR inhibition Covalent tethering of DNA template to Cas9 SECURE base editors Nme2Cas9 CRISPR interference...inhibition library Covalent tethering of DNA repair template to Cas9 Homology-directed repair (HDR) can be ...using a single-stranded oligodeoxynucleotide HDR template covalently attached to the Cas9-gRNA RNP complex...Candida albicans A new CRISPR interference (CRIPSRi) platform for targeted gene repression in Candida albicans...

...said. “It opens a new avenue of modifying and manipulating endogenous eukaryotic RNA.” Endless possibilities...Biological Engineering in March is fused to a translational repressor. In principle, however, designer PUF...PUF proteins for specific post-transcriptional regulation of endogenous RNA. J Biol Eng. 2014 Mar 1;8(1...(1):7. doi: 10.1186/1754-1611-8-7. PubMed.  Related Resources Addgene's Synthetic Biology Overview More...

...like manipulations, anomaly, and so on, differently than scientists would. For example, “manipulate” could...different topics of science, especially disease related topics. Neurodegenerative and brain disorders are...illicit tampering” to general audiences while “manipulate” could mean “worked with” or “changed” to scientists...

...Expanding your network of relationships early and often is the most effective tactic a scientist in training...result of existing professional (and personal) relationships. However, most job seekers will and should apply...after the academic bench. Utilizing existing relationships as part of the job application process can dramatically...you can use alumni databases, friends, and even relatives to network your way to a hiring manager. Learn...