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  1. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...Addgenie Mary Gearing contributed to the content of this article. Biosensors (‘biological sensors’) are...separated by CaM and M13. When calcium binds, it triggers CaM/M13 interaction that increases FRET transfer...

  2. Sensing Neuronal Dopamine

    Type
    Blog Post
    ... tool to detect and image DA in live animals (Patriarchi et al., 2018). Similar to calcium and glutamate...PMID: 4403305. PubMed Central PMCID: PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging ...

  4. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ... functions they may induce?  Fc receptors are intriguing from a therapeutic standpoint. Engineered monoclonal.... (2008). The immunoglobulin constant region contributes to affinity and specificity. Trends in Immunology...

  5. Hot Plasmids - October 2022

    Type
    Blog Post
    ...channelrhodopsins (KCRs), from a fungus-like protist Hyphochytrium catenoides (Govorunova et al. 2022). The authors...not just because of the title!) The Myc-tag is a string of 10 amino acids, EQKLISEEDL, that can be incorporated...

  6. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...for sale, and photos of the Agar Art competition entries were displayed in a gallery on the second floor... to find out more about our free deposit and distribution service! Did you see a must-have plasmid during...

  7. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...RFP, no FP). The vectors each contain two SfiI restriction sites with different overhangs depending upon...Cancer Center presented results from a clinical trial using the Sleeping Beauty transposase as a nonviral...

  9. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...one step involves boiling the glass powder in 50% nitric acid, but I never let that stop me. I only had ...glass slide using only surface tension.  It's a bit tricky to pour the right amount so the agarose doesn’t...

  13. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...This post was contributed by Richi Sakaguchi from Kyoto University, and Marcus N. Leiwe and Takeshi Imai...copies per cell per color, following a Poisson distribution. To overcome the limited expression levels of...

  15. The Strength of Story Telling

    Type
    Blog Post
    ... This post was contributed by guest blogger Jack Leeming Editor of Naturejobs, part of Nature magazine...dialogue specifically about the project and contributions, rather than getting defensive or getting into...

  16. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...fragments that recognize the endogenous IgGs (Lu and Partridge, 1998). After washing away unbound fragments, ...https://doi.org/10.1369/0022155413511620  Lu QL, Partridge TA (1998). A New Blocking Method for Application...

  17. What the HEK?

    Type
    Blog Post
    ...HEKs have an unusual ploidy and are considered hypotriploid: most chromosomes are represented by two to ...cells may be particularly useful due to their intrinsic properties. HEK-293 cells are tumorigenic, ...

  18. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...fantastic fodder for science communication. The trick is to find one or two roadblocks that sparked creativity...makes them want to know more. This could be by outright stating the big-picture problem, by asking your...

  19. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...suggest topics you’d like to see on the blog or to contribute ideas for your own guest posts by e-mailing us...generate knockouts, but how does it work? Guest contributors David Wyatt and Dale Ramsden from UNC Chapel...
Showing: 481 - 500 of 882 results