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Showing: 481 - 500 of 557 results

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Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

Type

Blog Post

..., Dickerson KE, Xie S, Hon GC, Xuan Z, Zhang MQ, Shao Z, Xu J (2017) In Situ Capture of Chromatin Interactions...
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

Type

Blog Post

...the common antibody is heftier (~150 kDa) and Y-shaped because it contains two large heavy chains and ...
Polymerase Chain Reaction Overview and Applications

Type

Blog Post

... in the early 1980s by Kary B. Mullis who later shared a Nobel Prize in Chemistry for his work. Since ...
Hot Plasmids: Winter 2025

Type

Blog Post

...If they do (or if they don't!), please consider sharing your experience with other researchers by contributing...
Degrading DNA with Cascade-Cas3

Type

Blog Post

.... S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R., Brouns, ...
Common Injection Routes in Mice

Type

Blog Post

... extra prep, as the injection site area must be shaved first. Restraint: anesthesia Location: between ...
Production of Virus in Insect Versus Mammalian Cells

Type

Blog Post

...protocols Viral Plasmids and Resources References Shaw, G., Morse, S., Ararat, M., & Graham, F. L. (2002...
Antibodies 101: Conventional vs Spectral Flow Cytometry

Type

Blog Post

...those markers are identical and completely indistinguishable. This tool is of particular value when designing...
Antibodies 101: Flow Compensation

Type

Blog Post

...She is currently a research technician in Arlene Sharpe's lab at Harvard Medical School. More resources...
PhD Applications After COVID

Type

Blog Post

...Every student about to graduate shares the same nightmare: the question “So, what comes next?” For those...
Truncated gRNAs for Regulating Gene Expression

Type

Blog Post

...26344044. PubMed Central PMCID: PMC4666719. 4. Mali, Prashant, et al. "RNA-guided human genome engineering via...
Plasmids 101: Knockout/Knock-In Plasmids

Type

Blog Post

...knockout animals or cell lines, please consider sharing them by depositing with Addgene! Figures were ...
AAV Vector Quality Control: Going the Extra Mile with NGS

Type

Blog Post

...do we make sure to catch all of these potential mishaps before distributing the vectors? Viral genome sequencing...
Cloning Mammalian Cells with the Agarose Method

Type

Blog Post

...obtaining antibiotic-resistant colonies, use a Sharpie to circle 12-48 individual colonies by (carefully...
Addgene's Cancer Collection Pages Connect the Research Community

Type

Blog Post

...therapeutically due to their unusually “smooth” shape and lack of “druggable” domains. In other words,...
Tips for Using BLAST to Verify Plasmids

Type

Blog Post

...sequencing results or comments on our suggestions? Share your thoughts here to help other labs speed up their...
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

Type

Blog Post

...Addgene as a professional public-benefit partner who shares our core values." Addgene spoke with Endy more ...
Capturing a Moment with Marc Zimmer’s “The State of Science”

Type

Blog Post

...transparent about his own biases--many of which will be shared by fellow scientists--but that doesn’t make his...
Plasmids 101: Choosing an Antibiotic Resistance Gene

Type

Blog Post

...period after transformation (the dreaded 60 minutes shaking at 37 degrees) can be shortened since the plated...
Getting to Know Expansion Microscopy

Type

Blog Post

... actin filaments or microtubules. This issue is shared by other SRM techniques and can be alleviated by...

Showing: 481 - 500 of 557 results

Results per page:

Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

Polymerase Chain Reaction Overview and Applications

Hot Plasmids: Winter 2025

Degrading DNA with Cascade-Cas3

Common Injection Routes in Mice

Production of Virus in Insect Versus Mammalian Cells

Antibodies 101: Conventional vs Spectral Flow Cytometry

Antibodies 101: Flow Compensation

PhD Applications After COVID

Truncated gRNAs for Regulating Gene Expression

Plasmids 101: Knockout/Knock-In Plasmids

AAV Vector Quality Control: Going the Extra Mile with NGS

Cloning Mammalian Cells with the Agarose Method

Addgene's Cancer Collection Pages Connect the Research Community

Tips for Using BLAST to Verify Plasmids

Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

Capturing a Moment with Marc Zimmer’s “The State of Science”

Plasmids 101: Choosing an Antibiotic Resistance Gene

Getting to Know Expansion Microscopy

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