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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 773 results for: Dos

Showing: 481 - 500 of 773 results

  1. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...selection: labeling markers that your cells of interest do not have, and selecting for the cells that are not...cytosolic proteins alongside the intranuclear ones. You do not need to use a separate fix/perm cytosolic procedure...

  2. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...RGD-binding integrins at non-ligand-binding sites and do not interfere with integrin function. Overall, the...function of mouse αVβ3, again the first known reagent to do so. The 15 hybridoma-derived antibodies fall into...

  4. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ... started!  I learned a lot about creative ways to do science communication and outreach - if you'd like...

  8. Antibodies 101: Validation

    Type
    Blog Post
    ...little to no signal in the liver sample, which you do (C), so your antibody is validated! Genetic Strategies.... The simplest version would be to use cells that do not express your protein of interest endogenously...

  10. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...less than an hour. However large proteins typically do not transfer as well using this method.  Finally,...overexpression systems) while negative controls are those that do not naturally express the protein or have had gene...

  12. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...and then derive mice from these engineered ESCs. To do so, the engineered ESCs would be injected into developing...mechanisms rush in to repair the damage. If all they can do is stitch the two broken ends together through non-homologous...

  17. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...protein present on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid...multiple target genes in Escherichia coli. Koffas Do you have suggestions for other plasmids that should...pyogenes EGFP Zoldos pdCas9-DNMT3A-PuroR 71667 Mammalian U6 yes, methylation S. pyogenes Puro Zoldos pX330-U6... Mammalian U6 yes, methylation S. pyogenes Puro Zoldos pBLO1811_Cas9_noNLS_human 74490 Mammalian U6 yes...
Showing: 481 - 500 of 773 results