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We narrowed to 771 results for: Dos

Showing: 481 - 500 of 771 results
  1. Molecular Biology Reference

    Type
    Guide
    ... can be converted back to a regular base and thus do not halt the reaction. Illumina NGS, therefore does...is important to note that NGS platforms in general do not require a specific primer for your DNA of interest...
  2. Antibodies 101: Validation

    Type
    Blog Post
    ...little to no signal in the liver sample, which you do (C), so your antibody is validated! Genetic Strategies.... The simplest version would be to use cells that do not express your protein of interest endogenously...
  3. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...less than an hour. However large proteins typically do not transfer as well using this method.  Finally,...overexpression systems) while negative controls are those that do not naturally express the protein or have had gene...
  4. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...and then derive mice from these engineered ESCs. To do so, the engineered ESCs would be injected into developing...mechanisms rush in to repair the damage. If all they can do is stitch the two broken ends together through non-homologous...
  5. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...species. Some Cas proteins, like LbCas12a or AsCas12a, do not require a tracrRNA. sgRNA: “Single-guide RNAs...
  6. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...recommended for most imaging applications. What can you do with SunTag? Tanenbaum et al. performed a variety...
Showing: 481 - 500 of 771 results