We narrowed to 769 results for: Dos
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TypeBlog Post... started! I learned a lot about creative ways to do science communication and outreach - if you'd like...
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Addgene's Top Blog Posts from 2020
TypeBlog Post...methods to quantify DNA? Here we cover five methods to do this. 5. Plasmids 101: Plasmid Incompatibility by... -
Management for Scientists: Seeking Feedback
TypeBlog Post...about changes influenced by feedback. If you don't do this, employees will stop providing feedback pretty... -
Plan Your Experiment
TypeGuide...model your specific disease or process of interest. Do you want to: Generate complete and permanent loss...percentage of DSBs are still repaired by NHEJ. How do you determine that your desired edit has occurred... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...limit, some like prime editing's PE2 enzyme do not. So how do you fit a big gene into a tiny vector like... -
Antibodies 101: Validation
TypeBlog Post...little to no signal in the liver sample, which you do (C), so your antibody is validated! Genetic Strategies.... The simplest version would be to use cells that do not express your protein of interest endogenously... -
Antibodies 101: The Basics of Western Blotting
TypeBlog Post...less than an hour. However large proteins typically do not transfer as well using this method. Finally,...overexpression systems) while negative controls are those that do not naturally express the protein or have had gene... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post...and then derive mice from these engineered ESCs. To do so, the engineered ESCs would be injected into developing...mechanisms rush in to repair the damage. If all they can do is stitch the two broken ends together through non-homologous... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog Post...repair, these methods do not involve double strand break (DSB) formation and do not require donor DNA,... -
Phage Directory: From Phage Therapy to a Repository of Phage Information
TypeBlog Post... infect and then kill bacteria they enter. But to do so, the phage must recognize a specific part on the... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog Post...years of incredible success, and for believing, as we do, that sharing speeds science. This year, Chonnettia... -
Easing the Protein Purification Process with pCri
TypeBlog Post...later. Protein purification specialists recommend doing some research, notably determining the subcellular... -
Inside Addgene's 10 Year Anniversary Gala
TypeBlog Post...business partners, without whom Addgene could not do what it does. Melina talked about her grandfather... -
Supporting Rare Disease Research with BeHeard 2017
TypeBlog Post...much international attention. Their small numbers do not, however, make these patients suffer any less... -
SunTag and Fluorescent Imaging
TypeBlog Post...recommended for most imaging applications. What can you do with SunTag? Tanenbaum et al. performed a variety... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog Post...you high-quality educational resources to help you do better research. CRISPR is an incredibly fast-moving... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog Post...sharing impact grow beyond COVID-19. But, we can’t do this alone. If you have deposited plasmids at Addgene... -
Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot
TypeBlog Post...arms so that they can hold and hug items closer and do so more animatedly. To communicate that sense of ... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog Post...degrading the tissue (Radtke et al., 2020, 2022). I do mean highly multiplexed: you can image over 65 different... -
Addgene is Expanding Our Viral Vector Service!
TypeBlog Post...with the request in your Addgene account. Orders do not need to be placed immediately upon request approval...