...methods to quantify DNA? Here we cover five methods to do this. 5. Plasmids 101: Plasmid Incompatibility by...

... started!  I learned a lot about creative ways to do science communication and outreach - if you'd like...

...remember to step back and ask, "Why did I start doing this in the first place?" Many thanks to our guest...

...about changes influenced by feedback. If you don't do this, employees will stop providing feedback pretty...

...less than an hour. However large proteins typically do not transfer as well using this method.  Finally,...overexpression systems) while negative controls are those that do not naturally express the protein or have had gene...

...limit, some like prime editing's PE2 enzyme do not. So how do you fit a big gene into a tiny vector like...

...little to no signal in the liver sample, which you do (C), so your antibody is validated! Genetic Strategies.... The simplest version would be to use cells that do not express your protein of interest endogenously...

...and then derive mice from these engineered ESCs. To do so, the engineered ESCs would be injected into developing...mechanisms rush in to repair the damage. If all they can do is stitch the two broken ends together through non-homologous...

... infect and then kill bacteria they enter. But to do so, the phage must recognize a specific part on the...

...later. Protein purification specialists recommend doing some research, notably determining the subcellular...

...business partners, without whom Addgene could not do what it does.  Melina talked about her grandfather...

...species. Some Cas proteins, like LbCas12a or AsCas12a, do not require a tracrRNA. sgRNA: “Single-guide RNAs...

...Your next cool experiment requires some AAV.  Where do you start? Plasmids of course! You just need three...

...degrading the tissue (Radtke et al., 2020, 2022). I do mean highly multiplexed: you can image over 65 different...

...arms so that they can hold and hug items closer and do so more animatedly. To communicate that sense of ...

... the fantastic work this Broad Institute lab is doing creating well-optimized CRISPR screening technology...

...you high-quality educational resources to help you do better research. CRISPR is an incredibly fast-moving...

...much international attention. Their small numbers do not, however, make these patients suffer any less...

...recommended for most imaging applications. What can you do with SunTag? Tanenbaum et al. performed a variety...

...sharing impact grow beyond COVID-19. But, we can’t do this alone. If you have deposited plasmids at Addgene...