We narrowed to 771 results for: Dos
- 
  TypeBlog Post...remember to step back and ask, "Why did I start doing this in the first place?" Many thanks to our guest...
- 
  Molecular Biology ReferenceTypeGuide... can be converted back to a regular base and thus do not halt the reaction. Illumina NGS, therefore does...is important to note that NGS platforms in general do not require a specific primer for your DNA of interest...
- 
  Management for Scientists: Seeking FeedbackTypeBlog Post...about changes influenced by feedback. If you don't do this, employees will stop providing feedback pretty...
- 
  Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated VirusTypeBlog Post...limit, some like prime editing's PE2 enzyme do not. So how do you fit a big gene into a tiny vector like...
- 
  Antibodies 101: ValidationTypeBlog Post...little to no signal in the liver sample, which you do (C), so your antibody is validated! Genetic Strategies.... The simplest version would be to use cells that do not express your protein of interest endogenously...
- 
  Antibodies 101: The Basics of Western BlottingTypeBlog Post...less than an hour. However large proteins typically do not transfer as well using this method. Finally,...overexpression systems) while negative controls are those that do not naturally express the protein or have had gene...
- 
  CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9TypeBlog Post...repair, these methods do not involve double strand break (DSB) formation and do not require donor DNA,...
- 
  Generating Mouse Models Using CRISPR/Cas9TypeBlog Post...and then derive mice from these engineered ESCs. To do so, the engineered ESCs would be injected into developing...mechanisms rush in to repair the damage. If all they can do is stitch the two broken ends together through non-homologous...
- 
  Phage Directory: From Phage Therapy to a Repository of Phage InformationTypeBlog Post... infect and then kill bacteria they enter. But to do so, the phage must recognize a specific part on the...
- 
  2023 and 2024: Reflections and Looking AheadTypeBlog Post...years of incredible success, and for believing, as we do, that sharing speeds science. This year, Chonnettia...
- 
  Easing the Protein Purification Process with pCriTypeBlog Post...later. Protein purification specialists recommend doing some research, notably determining the subcellular...
- 
  Inside Addgene's 10 Year Anniversary GalaTypeBlog Post...business partners, without whom Addgene could not do what it does. Melina talked about her grandfather...
- 
  New CRISPR Web Resources and #12DaysofCRISPR RecapTypeBlog Post...you high-quality educational resources to help you do better research. CRISPR is an incredibly fast-moving...
- 
  Addgene is Expanding Our Viral Vector Service!TypeBlog Post...with the request in your Addgene account. Orders do not need to be placed immediately upon request approval...
- 
  Build Your CRISPR VocabularyTypeBlog Post...species. Some Cas proteins, like LbCas12a or AsCas12a, do not require a tracrRNA. sgRNA: “Single-guide RNAs...
- 
  Viral Vectors 101: Parts of the AAV Packaging PlasmidTypeBlog Post...Your next cool experiment requires some AAV. Where do you start? Plasmids of course! You just need three...
- 
  Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and IndustryTypeBlog Post...sharing impact grow beyond COVID-19. But, we can’t do this alone. If you have deposited plasmids at Addgene...
- 
  Supporting Rare Disease Research with BeHeard 2017TypeBlog Post...much international attention. Their small numbers do not, however, make these patients suffer any less...
- 
  SunTag and Fluorescent ImagingTypeBlog Post...recommended for most imaging applications. What can you do with SunTag? Tanenbaum et al. performed a variety...
- 
  IBEX Knowledge-Base: A Data Resource for Multiplex Tissue ImagingTypeBlog Post...degrading the tissue (Radtke et al., 2020, 2022). I do mean highly multiplexed: you can image over 65 different...