We narrowed to 957 results for: Cre

...paths. Check out Joanne's Reddit AMA Here are some concrete things you can do to develop those transferable...Manage a project involving multiple scientists, create and stick to timelines Be a mentor in a formal ...If opportunities don't exist in your department, create them and show your leadership skills too. Teach...are always excellent Technical knowledge and creativity Become a “thought leader,” develop a technical...

... drug discovery by big pharma (i.e. HTRF and LanthaScreen) (Degorce et al., 2009), measuring intracellular...are critical in setting up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET... biomolecules each containing multiple binding crevices that can nonspecifically soak up your fluorophore...donors (Fig. 1). Scores of FP variants have been created, thereby enabling a broad spectrum of donor fluorescence...strategies. FRET experimental design lends itself to creativity and innovation, valuable commodities in any NIH...

...COVID-19 research? We decided to give it a try, by creating a service for COVID-19 researchers. So far, we...to researchers worldwide. “COVID-19 has spread incredibly fast and without any notion of national boundaries...-19. “Open scientific sharing is important for creating research synergies and accelerating the translational... but we believe that by coming together, we can create a significant impact on both clinical care and ...

...including Andrea Radtke, PhD, and Ziv Yaniv, PhD, have created the IBEX Knowledge-Base, a community repository...from the Zenodo dataset.     Figure 2: Screenshot showing reagent entries with associated metadata...others in the IBEX community.   Figure 3: Screenshot showing recent topics in the IBEX discussion ...when designing the IBEX Knowledge-Base, seeking to create an iterative process that is constantly becoming...

... The MORF library is only one of 22 libraries, created from 14 deposits, that became available through...would be remiss not to mention that 2024 is an incredibly special year for Addgene: it marks our 20th anniversary...twenty years while seizing new opportunities to create even greater value for the scientific community...every Addgenie, thank you for twenty years of incredible success, and for believing, as we do, that sharing...

...kit size, it may be tempting to try increasing your input to increase your output. But, exceeding a kit’...their relative qualities and quantities. Image created in BioRender.com. Transformation Grade DNA...that you are left with pure plasmid DNA. Image created in BioRender.com. For the clean-up stage, plasmid...kit.   Do you want eco-friendly options? It’s no secret that labs produce a lot of waste. If you are looking...

... at eScience Labs, an educational company that creates hands-on science lab kits and digital curricula...writing is clear, Caitlin’s career plans weren’t concrete when she finished grad school at the University...their research from the bench to the classroom by creating the outline of a college level course based on...

...within content and business.  Joining the Addgene crew!  This pattern was consistent: everyone I spoke ...One of the bigger projects I was tasked with was creating and sustaining a biweekly report of our social...coworkers and that trust then translated into this incredible experience.  Of course, I wasn’t presenting to... research on loads of different best practices, create an email strategy, and make the occasional TikTok...

... to honor the creativity, passion, and dedication to sharing epitomized by the creators of these plasmids...distribute our plasmids via Addgene. Given the increasing popularity of CRISPR in the plant community, ... to send these small tokens of thanks for the incredible impact our depositors have had on Addgene and...

... then degraded by the cell.4.) The transposase creates a double strand break in the DNA and allows the...transposase gains its name from the fact that it was recreated from a defective transposase found in fish. This...transposases. Improvements have been made since then by increasing the consensus between a greater number of extinct...primary cells. In 2009, Zsuzsanna Izsvak’s lab screened many SB tranposase variants for hyperactivity ...

...and Benjamin Glick from SnapGene. SnapGene was created to meet a need. While there were software tools...the DNA molecules they were using. SnapGene was created to alleviate these problems through good software...control elements. Development of this tool required creating a database of common features, and devising rules...the beginning or end of the feature. With the increasing popularity of gene synthesis, many researchers...

...reminds us that, although fluorescent proteins are incredibly useful, chemical fluorophores can still be used...Dangerous Viruses Contributed by guest blogger Tessa Cressy from the labs of Dr. Elke Mühlberger and Dr. Rachel...CombiGEM and CRISPR for Combinatorial Genetic Screening CombiGEM is a technology that can be used to quickly...barcode small libraries of CRISPR gRNAs to use for screening experiments. Get the full details on how to make...

.... “They took a dividing yeast and did a genetic screen to identify the factors that regulate cell division...Leuven in Belgium. “That’s where I not only got incredibly compelled by wet bench molecular research, but... over the years. “Some of the walls we’ve built create inefficiencies that ultimately make innovation ...science slow,” Vandenberghe says. “The continuous creativity that Addgene takes to find ways to disrupt these...

...who are also students in AP biology classes Creating a podcast about genetics using dog breeds as an...structure, and intent to the messaging - while also creating space for people outside of the primary audience...your team?)  Defining your audience allows you to create common ground and understanding that you can use...Image courtesy of Ron Mader, via Flickr, under the Creative Commons license     ...

... of several SARS-CoV-2 variants coincide with increased vaccination efforts. So, how effective are the...Neutralization levels against spike pseudovirus decreases depending on the variant. The lowest levels of... assay to study SARS-CoV-2 spike variants. “We created a series of spike expressing plasmids which encode...different circulating variants. This allowed us to create pseudoviruses that represent lineages and variants...

...RspWYL1 increases both targeted and collateral RNA degradation by RspCas13d. RspWYL1 also increased EsCas13d...Cas13d, both groups used very general bioinformatic screens that looked for a CRISPR repeat array near a putative... WYL-domain-containing accessory protein WYL1 increases RspCas13d and EsCas13d cleavage activity The majority...

...application of an electrical charge to cells, to increase cell membrane permeability and thus transformation...DNA can be accidentally packaged into the newly created phages. Transfer of this DNA to another cell is...Viruses (AAV). Lentiviral and AAV can be used to create both transient cell lines, where a gene of interest...Electroporation on the other hand uses electrical pulses to create transient pores in the cell membrane that genetic...

...sequencing by NGS. Addgene’s QC scientists immediately screen these plasmids to confirm they contain the expected...of plates will have select samples prepared and screened by NGS to spot check the samples, the rest will...are requested by scientists, will immediately be screened by our QC scientists to confirm the plasmids contain...By leveraging our core strengths of intensive screening and the use of high throughput platforms, Addgene...

... preserving cell viability. Library delivery Screening libraries, large collections of biological independent...independent variables, are becoming increasingly popular tools with many applications. These libraries ...library cargo delivered are controllable. For many screening applications, it is essential to deliver a single...being explored as the delivery mechanism for increasingly popular nucleic acid-based vaccines. These vectors...

...of Producing Monoclonal Antibodies, 1999). This creates an immortal cell population that will continue ...hybridomas for monoclonal antibody production. Image created with BioRender.com.   The antibody-producing... the genes from the hybridoma into a plasmid to create a recombinant antibody.     Figure 2: Changes...Generation of Hybridomas: Permanent Cell Lines Secreting Monoclonal Antibodies. https://www.ncbi.nlm.nih.gov...