...domain.   Check out our new Isotype trading cards!     References and resources References Janeway...

...of molecules including proteins, lipids, and carbohydrates, in this blog post we’ll focus on antibodies...

...DREADDs were engineered from members of the human muscarinic receptor family and are activated by small molecule...

... through Addgene. These plasmids can be used to carry out any biochemical and biophysical study involving...

...a transformed mesophilic, for example E. coli, carrying a plasmid expressing a protein of interest). Mesophilic...

...cancer variants derived from analysis of lung adenocarcinoma genomes (Lung Cancer Mutant Collection) or ...

... system from a lentiviral vector.  Lentiviruses carry a (+) strand RNA copy of the DNA sequence, which...

...  At some point in your long and illustrious lab career, you will likely accidentally leave your antibody...

...keep track of what works, and what does not work. Careful documentation will keep you on task and will prevent...

...result in a high number of background colonies carrying the unmodified plasmid. For insertions, it’s possible...

...differentiation between blue and white colonies. Take care in making your plates: X-gal is light and temperature...

...laboratory animals during in vivo research and the CARE guidelines for case reports are two sets of guidelines...

... die unless they have incorporated the plasmid carrying the required gene.  The table below lists some...

...dissolved in dH 2 O. *Carbenicillin can be used in place of ampicillin. Carbenicillin is more stable, so ...Protocols Cloning Protocols Introduction Plasmids can carry one or more antibiotic resistance genes, which confer...resistance to a specific antibiotic to the bacteria carrying them. The presence of an antibiotic resistance...that antibiotic - usually conferred by a plasmid carrying the antibiotic resistance gene. The following ...100 mg/mL 100 µg/mL Bleocin 5 mg/mL 5 µg/mL Carbenicillin* 100 mg/mL 100 µg/mL Chloramphenicol 25 mg/mL...

...proteins will refold once denatured, however, so take care when using this method.      Fig 2:  A, ...

... be done by either plasmid or virus. A plasmid carrying your cDNA can be targeted for integration at a...

...proteins of interest - the secondary antibody needs careful consideration before selection. If all the primary...

...of CRISPR-Cas9 by CASANOVA Article contributed by Cary Valley  Listen to the anti-CRISPR podcast segment...

...incredibly dedicated team. Many thanks to Tyler Ford, Caroline LaManna, Joanne Kamens, Lenny Teytelman, April...

...necessary result of the #MeToo movement. We are carefully attentive to genuine inclusion, but not worried...