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  1. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...stop for refreshment along the way? What ecosystem makes your heart sing with delight? From developmental...other scientists, or science journalists, or policy makers. The term editor covers a wide variety of careers...

  4. Plasmid Cloning by PCR

    Type
    Blog Post
    ...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding...restriction site (GAATTC) to the 5’ end of this primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC...

  6. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...?” and "What type of science careers would would make a good fit for me?" Whatever would I do next and...for sales and marketing jobs Like most choices we make, my job choices were mostly by chance. Luckily, ...

  7. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...terminal repeats (ITRs). ITRs are the elements that make an AAV plasmid an AAV plasmid.  Within the AAV ...and disruptive mutations and truncations that can make vector propagation and sequencing a headache. How...

  9. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...working in our laboratory! Don’t worry though, we make sure that everyone gets proper equipment and safety...engineer yeast to do similar things. The focus is to make yeast engineering accessible to everyone but these...

  10. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...Our Plasmids 101 eBook! One of the features that makes NEBuilder HiFi so accurate (apart from the high-...restriction enzyme digests to DNA assembly. What makes it so forgiving, is the incorporation of a 3’-5’...

  11. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...tools out there to visualize genomic loci, what makes CRISPR-Sirius unique? Unlike fluorescent in situ...predecessor CRISPRainbow, CRISPR-Sirius is brighter making it suitable for observing low-copy genomic loci...

  12. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...published in the same journals as those that did make deposits to Addgene.  Insomuch as different journals... argument that Addgene is achieving its goal of making plasmid-based science more accessible…and accruing...

  14. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...control for a specific marker in your experiment. To make an FMO sample, you take a subset of your experimental... is prepared very similarly to a FMO sample: you make a sample from a combined subset of your experimental...

  15. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...then inserted into the host's genome and is used to make more virus. The most studied (and used in the lab...also transduce dividing and non-dividing cells, making it conducive to targeting all cell types. Altogether...

  16. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ... Thus, the adjustable nature of viral libraries makes them the ideal vector for the job. Therapeutic ...immunogenicity, and cell-specific targeting features make them an ideal delivery system for human patients...

  17. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...and molecular biologists use to tinker with DNA. Making changes to a DNA sequence can help scientists identify... are a few drawbacks with these approaches that makes it challenging for users to achieve targeted diversification...

  18. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...If you’re using CRISPR to make a genome edit, how do you know if your CRISPR experiment was successful...different constructs for the reporter to work and to make the desired genome edit:  A donor plasmid that ...

  20. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...     The Trimmer Lab has continued to work to make the sequences of monoclonal antibodies publicly ...sequences from the Trimmer lab hybridoma collection and make them publicly available. The lab has also expanded...
Showing: 461 - 480 of 780 results