...guest blogger Derek Simon. The actual experiments you do will be determined by the topic you’re interested...Second, this may sound rudimentary, but before you do any type of behavior analysis, simply take a few ...

...Education & Tools menu Further down What happens if you do scroll down? You’ll find Blugene, as well as information...

...yourself, you’re probably wondering: What does it do and how does it work? LentiCRISPR v2 purpose As its...

...students next year! Does your lab or organization do work to help improve K-12 education? Let us know ...

...less likely to alter a protein’s properties. Why do we need new epitope tags? Short epitope tags like...

...alone. (Seriously, is there anything CRISPRs can't do?) Structural details All this, and scientists are...

...function and the correct gene of interest. So how do scientists sequence DNA? Sanger Sequencing In 1975...fluorescently tagged nucleotide. Because these special bases do not have a binding site for adding the next nucleotide... can be converted back to a regular base and thus do not halt the reaction. Illumina NGS, therefore does...is important to note that NGS platforms in general do not require a specific primer for your DNA of interest...

...of people willing to make that idea a reality! How do you share your science with the community? Addgene...

...it. 6. Edit your DNA sequence Finally, when you do see a miscalled peak, don’t be shy. Feel free to ...

...available through Addgene, and they hope others will do the same. “This isn’t the final version,” she said...

...tools in C. elegans and in Plasmodium. For those who do want to apply LITEs for the transformation of neurons...exposure. Third, the response can be graded with light dose and is fully reversible after light retraction. ...

...more. Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that should...separate non-integrating episomes to allow sorting and dosage tracking of reprogramming factors Fluorescent tagged...

...Davidson Fluorescent Protein Collection . Return to top Do you have suggestions for other plasmids that should...14437 mRFP-Rab5 Early endosomes RAB5A mRFP Ari Helenius 61801 GFP-Rab4B Early endosomes Rab4B AcGFP Gia Voeltz...49201 mCh-Rab5 Early endosomes Rab5 mCherry Gia Voeltz 49147 BFP-Rab5 Early endosomes Rab5 BFP Gia Voeltz...GFP-Rab5B Early endosomes Rab5B AcGFP Gia Voeltz 79802 pTag-RFP-C-h-Rab5a-c-Myc Early endosomes Rab5a TagRFP...pTag-BFP-C-h-Rab5a-c-Myc Early endosomes Rab5a TagBFP James Johnson 13050 DsRed-Rab5 WT Early endosomes RAB5A DsRed2 Richard...GFP-EEA1 wt Early endosomes EEA1 GFP Silvia Corvera 42635 TagRFP-T-EEA1 Early endosomes EEA1 TagRFP-T Silvia...61804 mCh-Rab7A Late endosomes Rab7a mCherry Gia Voeltz 61803 GFP-Rab7A Late endosomes Rab7a AcGFP Gia Voeltz...

... a single gene to cells. Still in bacteria, which do not produce BV, an additional gene coding for heme...proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular and molecular brightness...

...selection: labeling markers that your cells of interest do not have, and selecting for the cells that are not...cytosolic proteins alongside the intranuclear ones. You do not need to use a separate fix/perm cytosolic procedure...

...RGD-binding integrins at non-ligand-binding sites and do not interfere with integrin function. Overall, the...function of mouse αVβ3, again the first known reagent to do so. The 15 hybridoma-derived antibodies fall into...

...repair, these methods do not involve double strand break (DSB) formation and do not require donor DNA,...

...GFP, or RFP. Cells that express the construct but do not undergo recombination can be detected by immunostaining...transgene expressing either YFP or CFP, and cells that do not express Cre fluorescing with the default RFP....

...small probes that are entirely genetically encoded do not exist, but the development of non-natural fluorescent...functional heterotrimeric G-alpha subunit fusions that do not tolerate N- and C-terminal fusions (Adjobo-Hermans...

...USA and Europe, something that I would not able to do without a program like this. However, I realized ... she does research at UK in the same field that I do: prostate cancer. She has guided me in my next steps...