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Showing: 461 - 480 of 771 results
  1. COVID-19 Resources

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    Collection
    ...window) Content last reviewed on 26 September 2025. Do you have suggestions for other plasmids that should...al., 2020). Cathepsin L plasmids - a cysteine endosomal protease that triggers proteolysis of SARS-CoV...2020). Airway Ciliary Beating Affected by the Pcp4 Dose-Dependent [Ca2+]i Increase in Down Syndrome Mice...
  2. Addgene's Website Update

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    Blog Post
    ...Education & Tools menu Further down What happens if you do scroll down? You’ll find Blugene, as well as information...
  3. Keeping Up With CRISPR/Cas9

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    Blog Post
    ...alone. (Seriously, is there anything CRISPRs can't do?) Structural details All this, and scientists are...
  4. Let There Be LITE Plasmids

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    Blog Post
    ...tools in C. elegans and in Plasmodium. For those who do want to apply LITEs for the transformation of neurons...exposure. Third, the response can be graded with light dose and is fully reversible after light retraction. ...
  5. CRISPR Plasmids - Empty gRNA Vectors

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    Collection
    ...protein present on the plasmid. gRNA plasmids that do not co-express a Cas protein require a separate plasmid...multiple target genes in Escherichia coli. Koffas Do you have suggestions for other plasmids that should...pyogenes EGFP Zoldos pdCas9-DNMT3A-PuroR 71667 Mammalian U6 yes, methylation S. pyogenes Puro Zoldos pX330-U6... Mammalian U6 yes, methylation S. pyogenes Puro Zoldos pBLO1811_Cas9_noNLS_human 74490 Mammalian U6 yes...
  6. Antibodies 101: Beyond Surface Labeling

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    Blog Post
    ...selection: labeling markers that your cells of interest do not have, and selecting for the cells that are not...cytosolic proteins alongside the intranuclear ones. You do not need to use a separate fix/perm cytosolic procedure...
  7. Fluorescent Proteins 101: When GFP lets you down

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    Blog Post
    ...small probes that are entirely genetically encoded do not exist, but the development of non-natural fluorescent...functional heterotrimeric G-alpha subunit fusions that do not tolerate N- and C-terminal fusions (Adjobo-Hermans...
  8. Quick Guide to Near-Infrared Fluorescent Proteins

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    Blog Post
    ... a single gene to cells. Still in bacteria, which do not produce BV, an additional gene coding for heme...proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular and molecular brightness...
  9. An Integrin Antibody Toolkit from IPI

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    Blog Post
    ...RGD-binding integrins at non-ligand-binding sites and do not interfere with integrin function. Overall, the...function of mouse αVβ3, again the first known reagent to do so. The 15 hybridoma-derived antibodies fall into...
  10. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ... started!  I learned a lot about creative ways to do science communication and outreach - if you'd like...
Showing: 461 - 480 of 771 results