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Your Top Requested Plasmid in 2015!
TypeBlog Post...yourself, you’re probably wondering: What does it do and how does it work? LentiCRISPR v2 purpose As its... -
MassBioEd Career Exploration Day at Addgene
TypeBlog Post...students next year! Does your lab or organization do work to help improve K-12 education? Let us know ... -
Inntags: Innovative Protein Epitope Tagging
TypeBlog Post...less likely to alter a protein’s properties. Why do we need new epitope tags? Short epitope tags like... -
Keeping Up With CRISPR/Cas9
TypeBlog Post...alone. (Seriously, is there anything CRISPRs can't do?) Structural details All this, and scientists are... -
Science Communication Snapshot: DayCon 2015
TypeBlog Post...of people willing to make that idea a reality! How do you share your science with the community? Addgene... -
6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results
TypeBlog Post...it. 6. Edit your DNA sequence Finally, when you do see a miscalled peak, don’t be shy. Feel free to ... -
Designer PUF Proteins for Any RNA Target
TypeBlog Post...available through Addgene, and they hope others will do the same. “This isn’t the final version,” she said... -
Let There Be LITE Plasmids
TypeBlog Post...tools in C. elegans and in Plasmodium. For those who do want to apply LITEs for the transformation of neurons...exposure. Third, the response can be graded with light dose and is fully reversible after light retraction. ... -
An Integrin Antibody Toolkit from IPI
TypeBlog Post...RGD-binding integrins at non-ligand-binding sites and do not interfere with integrin function. Overall, the...function of mouse αVβ3, again the first known reagent to do so. The 15 hybridoma-derived antibodies fall into... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog Post... a single gene to cells. Still in bacteria, which do not produce BV, an additional gene coding for heme...proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular and molecular brightness... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...small probes that are entirely genetically encoded do not exist, but the development of non-natural fluorescent...functional heterotrimeric G-alpha subunit fusions that do not tolerate N- and C-terminal fusions (Adjobo-Hermans... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...GFP, or RFP. Cells that express the construct but do not undergo recombination can be detected by immunostaining...transgene expressing either YFP or CFP, and cells that do not express Cre fluorescing with the default RFP.... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post...selection: labeling markers that your cells of interest do not have, and selecting for the cells that are not...cytosolic proteins alongside the intranuclear ones. You do not need to use a separate fix/perm cytosolic procedure... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog Post...USA and Europe, something that I would not able to do without a program like this. However, I realized ... she does research at UK in the same field that I do: prostate cancer. She has guided me in my next steps... -
Microbes: Look, Listen, and Tell
TypeBlog Post... started! I learned a lot about creative ways to do science communication and outreach - if you'd like... -
Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton
TypeBlog Post...remember to step back and ask, "Why did I start doing this in the first place?" Many thanks to our guest... -
Addgene's Top Blog Posts from 2020
TypeBlog Post...methods to quantify DNA? Here we cover five methods to do this. 5. Plasmids 101: Plasmid Incompatibility by... -
COVID-19 Resources
TypeCollection...window) Content last reviewed on 26 September 2025. Do you have suggestions for other plasmids that should...al., 2020). Cathepsin L plasmids - a cysteine endosomal protease that triggers proteolysis of SARS-CoV...2020). Airway Ciliary Beating Affected by the Pcp4 Dose-Dependent [Ca2+]i Increase in Down Syndrome Mice... -
Molecular Biology Reference
TypeGuide... can be converted back to a regular base and thus do not halt the reaction. Illumina NGS, therefore does...is important to note that NGS platforms in general do not require a specific primer for your DNA of interest...