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We narrowed to 964 results for: Cre

Showing: 461 - 480 of 964 results

  1. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ...large number of constructs for high-throughput screening of PPIs. For example, Bischof et al. (2018) generated...generated an open reading frame (ORF) library to screen PPIs among 450 transcription factors in live fruit...organisms lags behind. BiFC can also be used to screen for small-molecule inhibitors of a particular PPI...Here, one of the endogenous PIPs is expressed at increasing levels alongside your BiFC constructs. If your...co-expressing one of the endogenous PIPs should decrease the BiFC signal in a dose-dependent manner. This...

  2. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...CRISPR, and the challenges of science becoming increasingly politicized. It would be nearly impossible for...all of these things contributed to the steadily increasing distrust the general public has in science and...self-promotion, and personality is playing an increasingly important role in science. The science produced...reserved Franklins and Prashers will find it increasingly difficult to compete (for funding, etc.) with...chapter on deep learning addresses the dilemma of creating a technology that could eventually replace scientists...

  3. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...Komagataella phaffii (a.k.a  Pichia pastoris) because it secretes fewer of its own proteins, making the purification...production of Fab fragments where variable chains are secreted into the periplasmic space to be oxidized and ...incubation time to maintain cell viability and increase protein production (Schwarz et. al., 2020). Harvest...   Figure 1: The steps of rAb production. Created with biorender.com   Another method of harvesting...contaminating bands or a few strong ones, consider increasing the number of washes performed during your harvest...

  4. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ... 2021). In fact, completion of Provost’s CURE increased students’ confidence in their research capabilities...projects have also been proposed as a mechanism for increasing diversity and inclusion in STEM disciplines (... Brownell, 2014; Duboue et al., 2022), as they increase student interest in scientific career paths while... Provost believes these networking experiences increase student enthusiasm, and research backs up that...undergraduate research experiences are a “CURE” that increases engagement by students and teachers. FEMS Microbiology...

  5. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...the templated DNA. Check out our blog post on increasing HDR efficiency to read more about optimizing ...pathways have been shown to be efficacious for increasing knock-in events (Yang et al., Int J Mol Sci)....culture media prior to and after Cas9 introduction increases HDR, just make sure to remove the inhibitors within...Early HR factors fused to Cas9 have been shown to increase HDR frequency by committing a break to performing...reading up on validating genome edits to find screening strategies for your experiment. Also learn about...

  6. CRISPR Antimicrobials

    Type
    Blog Post
    ...newest line of defense against bacteria. Created with BioRender.com.   The problem of antibiotic...may also induce cell death. CRISPR also enables increased specificity - rather than targeting a process ...mouse skin colonization model, phagemid treatment decreased the proportion of virulent S. aureus from 50% ...death. Image from Beisel et al., licensed under a Creative Commons Attribution 4.0 International License....rendering the population sensitive to antibiotics. Created with BioRender.com. Phages could be used to...

  7. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ... shalt create community to help Involve family. If you don’t have local family to help, create “fake” ... on the Society for Laboratory Automation and Screening (SLAS) Electronic Laboratory Neighborhood.   Work... to church, join a club, meet the neighbors and create a network of support to help with elder care, carpooling...partner – no kids. Get a massage – no guilt. Have recreational time with your friends – no spouses or kids....

  8. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...is possible with efficiency >70%. CRMAGE is an incredibly fast protocol, with only 5 hours incubation time...system that combines recombineering with CRISPR to create a system for scarless, iterative genome engineering... pathways within this genus, Huimin Zhao’s lab created two “pCRISPomyces” systems for use in Streptomyces...CRISPR in bacteria, they also led early efforts creating tools for CRISPRi and CRISPRa (Bikard et al., ... in Mycobacterial species, Sarah Fortune’s lab screened 11 other Cas9 orthologs to identify a more efficient... used to engineer the bee gut microbiome. They created a modular system using a broad host range plasmid...

  9. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...to solve this problem. This tool was initially created to support the CRISPR analysis needs of Synthego... sgRNAs. Since multiplexing is commonly used to create functional knockouts or large deletions, the ability...optimizing and no complicated steps to learn. For increased flexibility and scalability, the ICE software ...editing analysis Once the analysis is complete, a new screen appears with a graphical representation of the ...Data button on the bottom right of the analysis screen. ICE analysis details Contributions   The Contributions...

  10. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...careers. External collaborations are becoming increasingly important as more complex approaches to scientific...seminar to connect the imaging community and to create a platform for discussions and potential collaborations...postdoctoral work there are loads of opportunities to create communities, platforms, and to be a part of projects...your ideas and don’t be afraid to make something concrete happen. You may have more willing collaborators...make sure to share them with a short note - this creates a thoughtful way of interacting and is much more...

  11. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...fluorescent protein can be truncated to increase the FRET efficiency. Creating fusions at the N- and C- termini...double the size of the protein. In addition, it increases avidity because it doubles the number of binding...non-aliphatic amino acids. However, long linkers increase the chance that a fusion protein is cleaved by...Sheridan, Douglas L., et al. "A new way to rapidly create functional, fluorescent fusion proteins: random...

  12. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...for quantitative imaging, Ming Hammond’s group created Spinach2 using site-directed mutagenesis of Spinach...was cloned into bacterial expression vectors to create a library. Bacteria were transformed, and then ...for in vivo studies. Dimerization of Broccoli increases its fluorescence by about 1.8-fold, and may be...for greater contact with its RNA aptamer. This increased interaction between Corn and its small molecule... to TO1-biotin. Two of these variants exhibit increased brightness compared to Mango, and all are photostable...

  13. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...Dicer chops off the loop of hairpin structures to create a mature siRNA duplex. Fun fact! Endogenous miRNA...production starts with transcription of the miRNA to create pri-miRNA, which is then processed by a complex...shRNA and miRNA processing and silencing pathways. Created on BioRender.com.   Expressing shRNAs using...Highlighted blue regions show flanking genomic sequence. Created on BioRender.com. When it comes to the backbone...expression of specific mRNAs. shRNAs are a quick way to create stable knockdown cells lines, and you can usually...

  14. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ... used for a variety of research purposes, like creating stable cell lines, delivering CRISPR/Cas9 to cells...vector with an integrase-deficient viral vector. Created with BioRender.com. What are integrase-deficient... episomal forms of non-integrating transgenes. Created with BioRender.com; adapted from Banasik and McCray...regions of the genome (Schröder et al., 2002), thus increasing the risk for insertional mutagenesis. This integration...remember: You can never trust a lentivirus with secrets... Because they tend to insert themselves into ...

  15. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ... ligase The Roux Lab in 2016 optimized BioID to create BioID2, a smaller and improved promiscuous biotin...was its low sensitivity. Sensitivity could be increased with higher expression levels but for many fusion...until they identified a mutant with significantly increased sensitivity that did not affect the function or...miniTurbo were developed in the Ting Lab in 2018 to increase the speed of proximity labeling by promiscuous... BASU exhibits 1,000 fold faster kinetics and increases the signal to noise ratio 30-fold compared to ...

  16. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ... optimized Cas9 variants have been created in an effort to increase translation in a particular plant ... the development of disease-resistant crops to increase agricultural yield (Wang et al., 2016; Wang et... the intervening region into the plant genome. Created with BioRender.com. In recent years, deaminase-mediated...Addgene carries several useful CRISPR toolkits for creating plant expression plasmids, including plant CRISPR...

  17. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...highly recommend watching this entertaining video created by our friends on the Cambridge 2010 iGEM team ...of homology at their ends, which are typically created by PCR. Then, the fragments are incubated together... homology between neighboring fragments can be created via PCR amplification with primers that contain...typically fairly high, so there is usually no need to screen a large amount of colonies. Excluding the time ...advantages that Gibson assembly does not such as increased efficiency, shorter incubation times, or the ability...

  18. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...from Aschauer et al., 2013, Figure 1. Used under Creative Commons Attribution License. Within the nervous...can become methylated over time, resulting in decreased expression (Damdindorj et al., 2014; Nieuwenhuis...from Griffin et al., 2019, Figure 4. Used under Creative Commons Attribution 4.0 International License....vice versa. The total volume needed, however, increases with the size of the region you want to cover ... effects (e.g. recombinase-dependent vectors) increases with the amount of virus you add, so it is strongly...

  19. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...generated by SnapGene software.  Thanks to increasing affordability and accessibility, more researchers...by growing seed stocks at 30°C compared to 42°C. Screen multiple colonies to isolate the monomer Sometimes... 1984; Summers et al., 1993). In these cases, screening multiple bacterial colonies using one of the detection...DNA-forming sequences and WRN deficiency independently increase the frequency of base substitution in human cells... self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol Microbiol. ...

  20. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...promoters and create sophisticated patterns of UAS-driven gene expression. To further increase the complexity...system into a repressible system, Gossen and Bujard created the tetracycline-controlled transactivator (tTA...bacterial operons. A chimeric transactivator, cTA, was created by fusing CymR to the activation domain VP16. cTA...state, and co-expression of the inducer QF can increase QUAS driven expression by ~3,300-fold in Drosophila...
Showing: 461 - 480 of 964 results