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We narrowed to 644 results for: CAR

Showing: 461 - 480 of 644 results
  1. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...in a 50 mL conical tube and dispose after use. Carefully pour the filtered and diluted cell culture supernatant...Resin will appear compacted after centrifugation. Discard the flow through into a waste container. Add 5 ... 2 min to remove PBS. Repeat Steps 30–31 3x , discarding buffer from the collection tube each time. Place...Centrifuge at 1000 x g for 2 min to collect the sample. Discard column after use. Determine antibody concentration...min at RT . Centrifuge at 3100 x g for 8 min . Discard the flow through into a waste container. Add the...times to mix. Centrifuge at 3100 x g for 8 min . Discard the flow through into a waste container. Repeat...additional times to ensure a full buffer exchange. Discard the flow through into a waste container. Fill the...
  2. What the HEK?

    Type
    Blog Post
    ...machinery) along with the viral plasmid containing the cargo of interest, and HEKs will produce and package viral...
Showing: 461 - 480 of 644 results