...This post was contributed by guest blogger Gairik Sachdeva. Healthcare consulting is a fast-paced field...success. Or the client might need help with the intricate task of designing a strategic vision for a portfolio...styles, team situations, and personalities. Teams striving to achieve a lot of great output in a short amount...

...Oct 7, 2020 by Gabrielle Clouse. This post was contributed by guest bloggers Marcelle Tuttle and Alex Chavez...activation can be made inducible upon environmental triggers. This is done by creating a split dCas9 protein...activation are also reversible: once the environmental trigger leaves, gene expression is no longer activated....

... This post was contributed by guest bloggers Erik L. Snapp and Lindsey M. Costantini. "You underestimate...position requirements. For example, a KDEL ER retrieval sequence only functions at the extreme C-terminus...the fluorescence pattern is equivalent to the distribution of FP-fusion proteins. Our studies and those...

... This post was contributed by guest blogger, Tea Pavlek, Product Marketing Manager at sciNote. Today,...information is consolidated to one place, easily retrievable, searchable and traceable. However, switching...every lab, there are certain hierarchies and restrictions to data access. If these are important in your...

...such system is Brainbow 2.0, which uses Cre to trigger the inversion of two genes aligned head-to-head.... Another variation, Brainbow 2.1, uses Cre to trigger the inversion of two tandem sets of genes aligned...homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells." Cell 143.1 (2010)...

... of Biotin Attachment to Proteins; Symposium: Nutrition, Biochemistry and Molecular Biology of Biotin”...forgotten vitamin.” American Journal of Clinical Nutrition 75.2 (2002): 179–180. PubMed PMID: 11815306. 4...technology to improve cell adhesion on nanofibrous matrices.” Journal of Nanobiotechnology 13.1 (2015): 1–...

...committees. Having successfully navigated the trials and tribulations of the process, I’ve provided guidance ...ability to interview and sell a vision for your contribution to a Department's/Institution's success is the...

...Originally written by Marcy Patrick and Mary Gearing on Mar 12, 2015; updated by Christina Mork, Jul ...2023. DNA damage drives genome instability and contributes to cancer, premature aging, and other harmful...catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA. Richardson CD, et al. Nat Biotech....

...This post was contributed by Kyle Hooper at Promega. Researchers have been sharing plasmids ever since...Intracellular Calcium Sensor Yang, J. et al. (7) wished to trigger intracellular Ca++ release with an optogenetic ...specificity High sensitivity Easy expression Gives ratiometric data The result was a fusion protein called ...

...This post was contributed by guest blogger Greg Lohman, a biochemistry researcher at New England Biolabs...joining for recombinant plasmid construction (restriction cloning), adaptor ligation for next generation... can be achieved through a combination of the intrinsic selectivity of the ligase active site and careful...

...with fluorescent derivatives of its substrate trimpethoprim (TMP-tag) (Miller et al., 2005). Although this...Benzylguanine (BG) Many Easy eDHFR 159 Trimpethoprim  (TMP-tag) Few Easy HaloTag 295 ... V. W. (2005). In vivo protein labeling with trimethoprim conjugates: A flexible chemical tag. Nature ...

...fluoresce, and then passing the cells through an electric field to deflect them into appropriate containers...understanding the key roles of immune cells in triggering an immune response. New fluorescent proteins ... PubMed PMCID: PMC5024581. 15. Celli, Susanna, Béatrice Breart, and Philippe Bousso. "Intravital two-photon...

... This post was contributed by guest blogger Fredrik Wermeling, leader of a research group at the Centrum...to ensure that each gRNA has a good chance to contribute to the screen. In the end, this results in an...could majorly accelerate progression into clinical trials. Secondary screens  If you have generated a list...

...This post was contributed by guest blogger Stephanie Hays, a researcher at the University of California...age live in a state with at least two abortion restrictions that conflict with scientific evidence. We need...promote harmful policies that benefit polluting industries.In March, three climate scientists and an economist...

...systems are less popular in the lab. This can be attributed to the multi-Cas effector, Cascade. Developing...Developing tools using a Cascade complex is tricky, as you need to ensure every unit of the complex expresses... Tolstoy, I., Johnson, D. B., Koonin, E. V., & Quatrini, R. (2021). Evolution of Type IV CRISPR-Cas Systems...

...audiencia internacional, incluido trabajando con distribuidores de todo el mundo para ayudar a aumentar el ...international audience, including working with distributors across the world to help increase global access...

... Addgene will then apply for the license for distribution to the requester’s organization. A non-refundable...for rabies materials may be required for some countries and may not be required for others. You will be...

...of all our depositors here at Addgene — your contributions make the repository what it is. So we’re thrilled...crystal structures demonstrating “the structure is strikingly similar to the design model at atomic resolution...

... to develop resources for your community! To contribute plasmids made in your lab, see www.addgene.org... a colleague who would benefit from our free distribution services for their popular constructs, why not...

...will vote on the entries, select up to three winners, and reveal the winning entries on Twitter and Instagram...