...fluoresce, the Jaffrey lab asked:  Could an RNA aptamer do the same thing? Using the HBI derivative 3,5-difluoro...recognition of ions or particularly small molecules that do not generate an immune response. Aptamers can be ...

...fluorescence microscopy (Dani, et al., 2010). How do you overcome this resolution limit? Electron Microscopy...Prakash K, et al. 2022). So what’s a researcher to do if they want to understand the ultrastructural relationships...

... beads are particularly advantageous because they do not require centrifugation between washes which can...controls include knockout cell lines or tissues that do not express the protein. With this control set, you...

...expression. To design a viral vector system that could do both, Addgene depositor Didier Trono and collaborators... lentiviral system (Figure 2). The HIV genes that do remain are very important for viral production: Gag...

...Gene X in the untreated cells—but can we be certain? Do we know whether the plasmid delivered into the cells...negative controls that produce a similar product, but do not target an endogenous gene in your experimental...

...of necessary data requirements and likely will not do so (calling it a “sisyphean task” given the endless...Firstly, we rely on many other labs to teach us how to do experiments and understand their data. Likewise, ...

...other aspects of molecular biology. “What I'd like to do with the plasmids from Addgene is have the students...

...quantification so that you can focus on what you want to do with your plasmid without spending time and resources...

... Michael Früh submission, which we have entitled "Do You See What I See?" I was bored this weekend while...

...expect given the excitement surrounding anything to do with CRISPR, our most popular post in July (with ...

...comes from. Then consider the following questions: Do you need polyclonal or monoclonal antibodies? Will...direct or indirect detection method? If indirect, do you already have secondary antibodies available in...have identical features and epitopes, to ensure you do not validate the same antibody multiple times. You...antibodies will also have a source species. What conjugate do you want to use? Most secondary antibodies will be...buy small aliquots of many different antibodies and do batch validation up front rather than risk delaying...

...wild-type and engineered variants of Cas nucleases. In doing so, they also created a modular toolkit for Cas-...

...all the excitement and speculation, we thought we’d do a little digging and assemble a rough guide to the...

...to make all 12 possible base-to-base changes. How do base editors work? Base editing requires three elements...to use the deaminated DNA strand as a template. To do so, the lab used a Cas nickase, instead of dCas9....editors often produce a mixed population of edits, ABEs do not display significant A to non-G conversion at ...

...editor. Cas13 enzymes are advantageous because they do not require a PAM, and RNA targeting is potentially...flanking sequence (PFS) adjacent to the target, but many do not, showing the flexibility of this system.  As ...

...tool that is easy to use with other plasmids.  How do you know if your plasmids are incompatible? Scientists...will be based on sequence data. However, if these do not provide clarity, a wet lab transformation experiment...

...being published on our own topic. Only on occasion do we then have time to read about research from other...otherwise never learn from the valuable research we are doing. Browse the site, learn from others, share ideas...

... using site-directed mutagenesis of Spinach. In doing so, they identified the nucleotides that contribute...Just as vegetables come in a variety of colors, so do aptamers! Corn was recently described in 2017 (Song...

...Expression in Mammals AAV Titers: Where do they come from and what do they mean? A Match Made in Heaven: CRISPR...

...array of base editing reporters that can help you do just that. Monitoring APOBEC and Cas9-mediated editing...tested their hypothesis with GFP fluorescence. To do this they generated a silent GFP construct, that ...