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  3. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ... a homologous template provided by the sister chromosome. As cells take those two ends and put them back... a technology that was difficult for all but the most experienced users and puts it in the hands of pretty...

  4. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ... is further processed to isolate the antibodies. Most commonly, antibodies of the IgG isotype are used...When to stay away from polyclonal antibodies The most prevalent issue with polyclonal antibodies is their...

  7. RNA Extraction Without A Kit

    Type
    Blog Post
    ...during tissue collection and homogenization. The most widely known of these products is TRIzol® (also ...researchers can do to combat this issue. First and foremost, be mindful of your extractions- if you need really...

  8. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...dry lab scientists (or vice versa), then you have almost certainly experienced some of the hurdles I needed...needed to overcome.  I eventually pinpointed and (mostly) solved two main problems with Addgene’s cross ...

  9. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...relationships (also known as “networking”), one of the most common questions I receive is “Do I have to be on...to you (unless they’ve chosen to hide it). While most people use LinkedIn messaging, not everyone likes...

  10. Antibodies 101: Conjugation

    Type
    Blog Post
    ...conjugate class than fluorophores are enzymes. The most common enzyme conjugates are Horseradish Peroxidase...be achieved through several different mechanisms. Most take advantage of chemical groups intrinsically ...

  11. To Each HIS Own

    Type
    Blog Post
    ...isolate proteins, this blog will focus on one of the most common, purification based on the polyhistidine ...containing two or more adjacent histidines are present in most production systems and can bind and co-elute with...

  12. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...The shredder What sets Cas3 apart from Cas9 and almost all other Cas enzymes is its method of DNA cleavage...abilities   Choosing a Cas While Cas9 may be the most adaptable Cas currently available, the other Cas...

  13. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...sharing your science - which can drive away even the most enthusiastic of learners.  What contributes to cognitive...audience to take on, just to understand you at the most basic of levels.  Concepts This can be obvious, ...

  14. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ... a number of research and clinical applications. Most of the time, when someone speaks about an affinity...thing to go when downsizing an affinity reagent, most of the alternative reagents have reduced immunogenicity...

  15. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...was successfully used in conditions that are the most similar to yours. Take a look at the publications... You now know that application validation is the most important factor in antibody selection, but what...

  16. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ... 145,000 more quality-adjusted life years. While most of the results were positive, the analysis did find...et al. provide a table indicating the sex of the most commonly used cell lines appearing in American Journal...

  17. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...://pubmed.ncbi.nlm.nih.gov/32675376/ mGold, the most photostable yellow fluorescent protein to date By... as bright to mVenus, the parent YFP, and is the most photostable YFP to date. As such, mGold is expected...

  18. Hot Plasmids - October 2020

    Type
    Blog Post
    ...traditionally introduced in Caenorhabditis elegans on extrachromosomal arrays can lead to artifacts due to overexpression...flow cytometry. Cells grown in high IL-3 showed mostly blue fluorescence, indicating a quick cell cycle...

  20. Engaging with science and society at pgEd

    Type
    Blog Post
    ...well as schools, community events, churches or mosques. On occasion, we also get interviewed for print...fields of research, what often concerns them the most about research is its implications for their lives...
Showing: 421 - 440 of 740 results