...Find Cpf1 Plasmids at Addgene   Image attribution: Zetsche, Bernd, et al. "Cpf1 is a single RNA-guided...differing from that of Cas9 (3’-NGG) in both distribution and GC-content, which might improve editing ...directional gene transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer...

...This post was contributed by Nikolai Braun and Keira Havens, co-founders of Revolution Bioengineering...with very little money. So when did inspiration strike us so hard that we upended our lives and took this...things we could do for work, and they weren’t restricted to science jobs. Keira’s an optimist, I’m a realist...

...downstream flanking sequences to their input string (Figure 2). This string can then be used as input for the prediction...many genomic locations. In our study, we used the TRIP (Thousands of Reporters Integrated in Parallel; ...

...This post was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University....their germ cells (sets of genomic material are contributed by mother and father). While some mutations can...start working with live animals yet, Harvard’s DRSC/TRiP- Functional Genomics Resources website has in vitro...

...creating the Expression Systems table. Marcy Patrick contributed to the writing of this post. Additional Resources...Last updated Sep 30, 2020 by Marcy Patrick. CRISPR technology has been widely adopted for genome editing...

...default scoring matrix BLOSUM62, but you may want to check the description of the other matrices to see if ...laboratories that deposit their plasmids with us or other entries in NCBI’s numerous databases. As our repository...

...production, reliable expression, and easy distribution as DNA (Trimmer, 2020), you may be interested in using...Purification Protocol Addgene's Antibody Guide References Trimmer JS. Recombinant Antibodies in Basic Neuroscience...

...sgRNA-level metrics to assess library performance in viability screens. Based on these metrics the Brunello... CRISPRi libraries with 10 sgRNAs in the sgRNA metrics, confirming again that sgRNA design plays a significant...

...and relatively easy? Have all of these things contributed to the steadily increasing distrust the general...that “Addgene, with its easy, fast, and cheap distribution of the newest CRISPR-Cas plasmids, has been ...to thank fellow Addgenies Joanne Kamens, Marcy Patrick, Aliyah Weinstein, Fabienne Furt, and Quintin Marcelino...

...This post was contributed by guest blogger, Luke Lavis, a Group Leader at the Janelia Research Campus...synthetic fluorophores. Add a dash of DNA with an electrical jolt and cells become perfectly capable of synthesizing... D. Lavis! All images in this blog post were contributed by Luke D. Lavis. Luke D. Lavis is currently ...

...therapy. In 2003, VIRxSYS began the first clinical trial with a lentiviral vector. CD4+ T-cells from patients... lentiviral vectors have been observed in small trials for other diseases, including the common hematopoietic...monitoring and follow-up in lentiviral clinical trials. McGarrity GJ, Hoyah G, Winemiller A, Andre K, ...

...insert. Traditionally this has been done with restriction enzyme digest; however colony PCR can accomplish...you’re screening for positive clones! Tips and tricks from the bench Don't pick too large of a colony...Additional Resources on the Addgene Blog Learn to do Restriction Cloning Try SLIC cloning Learn about Gibson Assembly...

...competitions (ranging from cook-offs, to corn toss to trivia, and everything in between). Eleven years later...everything from kayaking, to field day, to relay triathlons, to 5Ks. Many of these examples we’ve done annually...are the sole event planner)! If you have a very intricate itinerary, there is a higher likelihood that something...

...specific DNA sequences. Thus, we've made some contributions in technology development in biology and hope...in these professional activities because I can contribute by, for example, helping to improve the papers...optimized for living for Japanese, which is not trivial, and the grant situation is currently much better...

... mYFP S65G; V68L; Q69K; S72A; T203Y; A206K Citrine S65G; V68L; Q69K; S72A; T203Y ECFP F64L; S65T...Fluorescent Protein Guide Note: A. Max Juchheim contributed to the writing of this article. ... Marcy Patrick...

...This post was contributed by Joe James from Binning Singletons. The sheer scale of a large conference...their meetings. Amanda Gunn ran randomized coffee trials for us at ASCB|EMBO 2019, matching Mentors with...ways. Scientific societies and conferences should strive to provide support to early career scientists, ...

...This post was contributed by Rory Macneil, founder of Research Space. There are many types of electronic... the ELN is reproducible, and only the highly restricted number of people who can access the ELN have ... the ELN arises here. Notwithstanding access restrictions based on permissions, however, in some cases...

...This post was contributed by Kutubuddin Molla, a Fulbright Visiting Scholar at the Pennsylvania State...strand, dramatically reducing the likelihood of detrimental indel formation. After binding with DNA, the ...editing causes the creation or destruction of any restriction enzyme (RE) recognition site, RE analysis of ...

... binding signal for Cas9 and this sequence is a strict requirement for Cas9-mediated DNA cleavage. The...below. Note: Jennifer Tsang and Mary Gearing contributed to the update of this post. References Abudayyeh...Wolfe SA, Sontheimer EJ (2018) NmeCas9 is an intrinsically high-fidelity genome-editing platform. Genome...

... the nucleus and modulate transcription of the mCitrine gene. mCherry is constitutively expressed. Cells... fluorescent proteins, they found that adding a triple nuclear localization signal or including fusions...CRISPRi-based synthetic oscillator, bistable network, stripe pattern-forming incoherent feed-forward loop. ...