... of selective reagent for 12 days with media exchanges every 3–4 days. After 12 days of selection, no ...adapted to a variety of target cell lines and selection markers. Note this assay requires staining resistant... 2: Replace media with fresh media containing selection reagent Days 3–14: Change media as needed Days...Colonies were allowed to grow in the presence of selective reagent for 12 days with media exchanges every...

... We also offer ready-to-use AAV preparations of select luciferase expression plasmids; these are noted...

...on Request service offers you even more options. Select from a vast catalog of eligible AAV plasmids and...

...Learn about selecting the correct pipettor and pipette tip, how to dispense liquids, and how to handle...experimental results. This protocol will guide you in selecting the correct pipette and pipette tip and using ...know the different parts of the pipette, how to select tips, and how to read a pipette, you’re ready to...display or the top of the plunger of the pipette. Select the pipette tip that is designed to fit your pipette...

...bacteria that do not contain it by artificial selection (i.e. growing the bacteria in the presence of ...addition of an antibiotic to this gel allows for the selection of only those bacteria with resistance to that...stable, so it is potentially more effective at selecting only bacteria containing the plasmids of interest...the antibiotic at too low a concentration for selection. Negative Result 2: Neither Strains Grows If neither...

... tRNA. Applications By making small changes in selected amino acids within a protein, any alterations ...

...most widely used method scientists employ to selectively move a specific piece of DNA from one plasmid...plasmid contains the expected size insert. By selecting the appropriate enzyme(s), one can either linearize...beginning your diagnostic digest, you will need to select a restriction enzyme or enzymes that cut your plasmid...

...for a wide range of applications, with videos for select protocols in the right-hand column. You can find...and use Watch the Video! Pipetting Learn about selecting the correct pipettor and pipette tip, how to dispense...pipette Watch the Video! Centrifugation Learn about selecting and using a centrifuge to separate different components...

...scientific research. Addgene's AAV are made from a selected set of plasmids that are currently in our repository...

...same technique can be used to move promoters, selectable markers, or any other DNA element between plasmids...restriction sites are present in a given sequence. When selecting restriction enzymes, you want to choose enzymes...information about restriction enzyme buffers). If you select enzymes that can function in the same buffer, it...

...min for proteins >150 kDa. Select Start Run. When the run is complete, select Done. Section 5: Blocking...Gel Transfer Device. Close the lid of the device. Select the desired method and make sure the parameters...

...high-throughput next generation sequencing (NGS). Select a restriction enzyme for digest that will cut the...propagation (origin of replication and antibiotic selection). This recombination, at a low rate, is not typically...Recombination is thought to be in part related to selective pressure. These recombined plasmids are much smaller...

...proteins, point mutations, deletions, etc.) involves selecting one or more unique oligonucleotide primers that...There are a number of factors to consider when selecting sequencing primers, including: Is the primer unique...

...bacteria that do not contain it by artificial selection (i.e. growing the bacteria in the presence of ...flask. Using a sterile pipette tip or toothpick, select a single colony from your LB agar plate . Drop ...

...the reaction mixture, and may provide counter-selection for negative clones as in the example shown here...removing the stuffer sequence and sacB counter-selection marker (see our restriction digest protocol )....

...sequence to be amplified (usually 18-21bp). When selecting restriction sites, you should use a DNA analysis... longer the expected PCR product is. You should select an annealing temperature based on the melting temperature...

...for 1 hour. Lithium Chloride (Option B): LiCl selectively precipitates RNA versus DNA or proteins. Add ...

...chemically by joining nucleotides together. One must selectively block and unblock repeatedly the reactive groups...

...Learn about selecting and using a centrifuge to separate different components in a liquid sample....

...adapted to a variety of target cell lines and selection markers. Note, because this protocol uses microscopy...