...PMCID: PMC307639. 2. Daiguan Yu, Hilary M. Ellis, E-Chiang Lee, Nancy A. Jenkins, Neal G. Copeland, and Donald...recombination system for chromosome engineering in Escherichia coli. PNAS 2000 97 (11) 5978-5983; May 16, 2000...

... target. There are a multitude of options for achieving success in a science policy career with many opportunities...organizations, working in student government, and teaching and mentoring others (see more in Figure 2). Starting...

...string of letters is a part of the word you’re searching for. Antibodies may recognize a linearized sequence...a slide, or whole organs. Because the tissue architecture is preserved, this technique allows you to not...

... mammalian cells. Type IV Type IV is the middle child (like me!) of CRISPR types — weird and poorly understood...involved in plasmid competition or can hijack machinery from other CRISPR systems to carry out various...

... inhibitor with a degradation tag (LVA tag) to achieve specific point mutations in E. coli. gRNAs are ...plasmids from this paper at Addgene. 4. Albers, Joachim, et al. “A versatile modular vector system for ...

...Cofsky et al., 2022; Osuka et al., 2018). Each matching DNA:RNA pair gives just enough stability for the...Molecular graphics and analyses performed with UCSF ChimeraX, developed by the Resource for Biocomputing, Visualization...

...easy for you to share plasmids. To achieve this goal, we will archive any plasmids you've deposited with...

...versatile, but it requires careful optimization to achieve the best efficiency. The type of edit, genomic ...Liu lab contributed an improved prime editing “architecture” that can be combined with any of the PE2-PE5...

...With both siRNA and shRNA, it can be difficult to achieve sufficient knockdown to enable robust assessment...S., Wright, A. K. A., Sousa, J., Weston, C. A., Chachi, L., Roach, K. M., & Bradding, P. (2019). Validation...

... can also download the software for use on any machine with OpenCL enabled hardware. This OpenCL dependency...genomes), then it is worthwhile buying a dedicated machine. On the offline front, there is a Python script...

...Gibson Type IIS Ligation Independent Cloning Oligo Stitching Molecular cloning or the creation of recombinant...Top Yeast-mediated Cloning and Oligonucleotide Stitching Yeast-mediated cloning is very similar in principle...advantage is the ability to perform oligonucleotide stitching, in which pieces of DNA that share no end homology...

...these common antibody applications.  Thanks for watching, and we hope you found the video radd-ically helpful...

...the lab? Or looking for ways to supplement your teaching? You’re in luck! Addgene’s Intro to the Lab Bench...

...prime editing systems through manipulating MMR machinery. On the fifth day of CRISPR, Addgene gave to me...

...DuanVisualizing Your Plasmid Request Data Author: Joachim Goedhart  Created in collaboration with Addgene...

... and tacos The Miller Lab at the University of Michigan decorated with a little bit of everything: frog...

...today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo. Check them out: https://t.co/uDEE9PsspD...

...mission and how they assess whether or not their achieving it. Chapter 5 - JEI's future - getting more involved...

...that will keep the lab running like a well-oiled machine. Lab tip #1: Glove trick Putting on lab gloves ...

...community, we've partnered with distributors in China, Korea, Japan, India, and Brazil.  We've also created...