... allows CRISPRi experiments in diverse bacteria Carol Gross’s lab recently deposited “Mobile-CRISPRi” ...

...provide great training for a number of alternative careers in science. In return, the public receives trustworthy...

... Carissa Fish...

...editing CRISPRi toolkit for Burkholderia Silvia Cardona’s lab recently developed a CRISPRi toolkit for Burkholderia...

...a culture of openness and trust that comes from caring about your colleagues on a personal level, and ...

...Bio-Rad, 1863005 DG8 cartridge, Bio-Rad, 1864008 DG8 gasket, Bio-Rad, 1863009 DG8 cartridge holder, Bio-Rad... Generating the Droplets Place a DG8 cartridge into the cartridge holder. Using a 2–50 µL multichannel...Cover the cartridge with the DG8 gasket, making sure that it is secure. Transfer the cartridge holder to... centrifuge and place on ice. Wipe down a DG8 cartridge holder with bleach and place it in the Biological...Add 16 µL of the master mix to each PCR tube. Be careful to dispense to the bottom of the tube without collecting...reaction mixtures into the middle wells of the cartridge. Add 800 µL of droplet generation oil to a polystyrene...

...molecular biology protocols here Visit the Addgene careers page ...

...RNPs to cleave substrate when the reaction was carried out at elevated temperatures. In both cases, GeoCas9...

...in addition to the Halloween contest, we’ve also carved pumpkins (yes, Blugene made an appearance there...

... Blog Resume Writing for Non-academic Science Careers 10 Steps to a Perfect Science Talk 5 Reasons to...

...that you are not interested in. You can use the carats in the datatable headers to sort alphabetically...

...25386923. PubMed Central PMCID: PMC4227841. 2. Young, Carissa L., Zachary T. Britton, and Anne S. Robinson. "...

... Caroline LaManna...

...the routine, I realized that the “9-5” was not as scary as it seemed. The transition into a traditional ...

...can create a significant impact on both clinical care and public health for this current COVID-19 pandemic...

...ways to improve delivery by increasing transgene cargo capacity, enhancing anterograde transport efficiency...

...professionally to network, learn more about alternative careers for scientists, and share cool science. Unfortunately...

...around Agrobacterium tumifaciens. Agrobacterium carries a “tumor-inducing” or Ti plasmid, which allows ...

...coworkers and doing something meaningful with my career. I can’t pinpoint exactly when I realized I ...

...preferences to help you identify proximal lines you care about. If you have cell lines without a certified...