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  1. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...Yamanaka himself, during his keynote address, appeared almost incredulous that the field has exploded so quickly...applications with the potential to save lives. While most scientists recognize and appreciate the importance...

  2. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...Postdoctoral Research Fellow with Pamela Silver.  Most types of biological motion (whether endocytosis,...cartoony explanation of R-bodies, check out this video. Most types of biological motion (whether endocytosis,...

  3. How to Lead a Great Meeting

    Type
    Blog Post
    ...meeting with no idea why you are there. Make the most of everyone’s time by considering the level of meeting...to meet monthly, but we found our meetings took almost two hours and we felt out of touch. By moving to...

  4. Components of CRISPR/Cas9

    Type
    Blog Post
    ...Updated Mar 26, 2020. At their most basic level, CRISPR/Cas9 genome editing systems use a non-specific...crRNAs from pre-crRNAs derived from CRISPR arrays. In most systems used for CRISPR-mediated genome editing,...

  5. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...pathways with sophisticated gene expression controls. Most cell types do not respond to low-intensity light...number of plasmids, with one RR (ompR) encoded chromosomally. Leakiness was also an issue; some promoter ...

  6. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...fluorescent proteins in common use (particularly not the most recently published ones), they can be a good starting...proteins the green and red proteins are generally the most detectable and so should be used to tag your least...

  8. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...goals. These first hires will set the tone and atmosphere that future hires will look up to." She also ... "Running Your Lab" booklet. What has been the most surprising thing about becoming a PI? Tom Ellis ...

  11. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...educational resources. Filters We identified the most useful kinds of information per resource category... details that make our new search engine unique. Most importantly, with this new implementation, we laid...

  13. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    .... While some developers had science backgrounds, most did not. It was my job to translate complex lab ...front-end and back-end coding frameworks to use. I mostly sit and listen to stay informed on the details,...

  14. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...promoters Chemically regulated promoters are among the most common inducible promoters. The positive inducible... agents to allow promoter activation. One of the most commonly used prokaryotic promoters is the negative...

  15. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...Overall, these approaches have two main drawbacks: 1) Most rely on transfection of more than one vector to ...lentiviral transduction, and AAV transduction.  In most of Zetsche et al’s experiments, a single vector ...

  16. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...nuclear envelope, which occurs during mitosis. The most well-known retrovirus is HIV. Lentiviruses are ... Gene Therapy with AAV Figure out How to Get the Most from Your Lentiviral Transduction Additional Resources...

  17. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...the light microscope has been a powerful tool in almost all disciplines. In biology, innovative new imaging...Since I started my PhD, I have been convinced that most microscopy techniques can be replicated with commercially...

  18. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...available to the research community.    Validation For most cloning projects, the next step would be to validate...digest of the PCR products prior to cloning, which in most cases left the functional light chain intact, the...

  19. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...integrated into the host genome upon viral transduction. Most transfer plasmids available from Addgene were derived...can be. Plasmids that encode SIN γ-retrovirus lack most of the U3 region of the 3’ LTR and are primarily...

  20. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...intrigued by the tRNA processing machinery present in almost every living organism. Precursor, or pre-tRNAs ...levels of gRNA expression. Since tRNAs are among the most abundant molecules in a cell, this system is clearly...
Showing: 401 - 420 of 740 results