...streptavidin-binding strep-tag and, more recently, OLLAS (Escherichia coli OmpF linker and mouse langerin) and Sun ...Spaghetti Monster is a symbol of opposition to the teaching of intelligent design in public schools and was...

...10.1126/science.aaf5573 Broughton JP, Deng X, Yu G, Fasching CL, Servellita V, Singh J, Miao X, Streithorst...Miller S, Pan C-Y, Guevara H, Wadford DA, Chen JS, Chiu CY (2020) CRISPR–Cas12-based detection of SARS-CoV...

...particular topic. Alternatively, maybe you’re researching a topic for which no relevant or recent review...Blog Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing Tips for Improving Your...

...Videos Over at the Addgene Youtube Channel   After watching these trends for a few years, I decided to look... brief introduction to the research and, after watching the video to determine if you’d like to learn ...

...Reddit Science is heavily moderated. I started watching the questions and comments go up early in the ...mechanics of doing the AMA were interesting. I started watching the questions and preparing answers early in the...

...hope that a similar reversal may be possible for children and adults with Rett Syndrome.  Since these discoveries...these approaches, such as delivery of the editing machinery to the brain, minimizing off-target editing, and...

...rate dependence of transfer RNA abundance in Escherichia coli." The EMBO journal 9.13 (1990): 4359-4366...rate of full‐length human proteins expressed in Escherichia coli." Protein Science 19.7 (2010): 1312-1326...

...Grover M. Hutchins. "Henrietta Lacks, HeLa cells, and cell culture contamination." Archives of pathology...

...the quantitative increase in fluorescent signal achieved when utilizing an optimized moxFP as opposed to...Griffith, her research focuses on the replication machinery of Kaposi’s sarcoma-associated herpesvirus.   ...

...protein products, which can prevent the culture from reaching the desired density within a reasonable time-frame...cystosolic protein products, but this is not always achievable. Proteins which contain hydrophobic regions or...

...biotin, avidin. Avidin was aptly named for the chicken, “Avian”, egg it was purified from. Snell later...Southern/Northern blotting Cell surface labelling Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated...

...retrievable, searchable and traceable. However, switching from paper to an electronic lab notebook requires...within your lab? In every lab, there are certain hierarchies and restrictions to data access. If these are...

...or difficult to create this list. Start BEFORE searching for a faculty position. This is important to ensure...seem, you should consider it if it will help you achieve your research goals, even if that means saying ...

...opening the door for immunostaining analysis. To achieve even cell labeling, they generated farnesylated..., Zimmerman S, Ciruna B, Sanes JR, Luchtman JW, Schier AF. Development. 2013 Jul;140(13):2835-46. doi:...

...Adeno-associated virus (AAV) is a widely-used tool for achieving in vivo expression of chemogenetic receptors, ... our AAV guide . FLEx Vectors are also used to achieve cell-specific expression of AAV-encoded chemogenetic...Mathews WB, Sysa-Shah P, Rodriguez LA, Ellis RJ, Richie CT, Harvey BK, Dannals RF, Pomper MG, Bonci A, ...M-R, Tomita Y, Nakahara J, Suhara T, Takada M, Higuchi M, Jin J, Roth BL, Minamimoto T (2020). Deschloroclozapine...Mouravlev A, Carpenter JC, Snowball A, Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D,...

... particularly interested in, but I’d recommend watching the whole livestream for a more holistic understand...future work cost both time and money with estimates reaching in the billions of dollars (Freedman et al 2015...

...Although there are different ways to ultimately achieve success, we find it is usually faster and easier...large number of different cells. Promoters such as chicken-beta-actin or CAG can drive strong early gene expression...

...need! How does it work? There are several ways to achieve multiplex IF. One of the most common approaches...the sample, either by chemical treatment or photobleaching or by stripping out the antibodies, then start...

...alternative sequencing approaches have been developed. Cas ChIP-Seq, where only DNA sites bound by Cas are sequenced...for an activator or repressor Cas. Similarly, Cas ChIP-Seq would also identify all DNA binding sites within...

...rural health clinic in Ghana. My role involved teaching the nursing students and community health workers... world. The research project was hard; I was researching the epidemiology of breast cancer and I loved...