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  1. Call for Guest Bloggers

    Type
    Blog Post
    ... Replica plating Anti-CRISPR Retroviral vectors Career development for scientists A new plasmid tool your...

  3. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ...efforts, you’ll find new ways to learn about science careers, lab protocols, and, of course, plasmids. Today...

  9. Editor's Choice, October 2016

    Type
    Blog Post
    ...Engineering the Plant Genome Using CRISPR/Cas9 Healthcare Consulting: A Door to the Business of Life Sciences...

  12. AAV ddPCR Titration

    Type
    Protocol
    ...Bio-Rad, 1863005 DG8 cartridge, Bio-Rad, 1864008 DG8 gasket, Bio-Rad, 1863009 DG8 cartridge holder, Bio-Rad... step 9 to the NTC tube. Place a DG8 cartridge into the cartridge holder. Using a 2–50 µL multichannel...Cover the cartridge with the DG8 gasket, making sure that it is secure. Transfer the cartridge holder to... centrifuge and place on ice. Wipe down a DG8 cartridge holder with bleach and place in the Biological...reservoir. Using a 20–200 µL multichannel pipette, carefully add the dilution buffer to the dilution plate ...Add 20 µL of the master mix to each PCR tube. Be careful to dispense to the bottom of the tube without collecting...reaction mixtures into the middle wells of the cartridge. Add 800 µL of droplet generation oil to a polystyrene...

  13. Kit Free RNA Extraction

    Type
    Protocol
    ... access to a refrigerated centrifuge, you can carefully bring a centrifuge into a cold room for centrifugation...interphase layer, and a top, aqueous layer. Take care to not disturb or collect the interphase layer with...be small, RNase-free Glycogen may be used as a carrier to facilitate RNA precipitation. This does not ...Centrifuge for 20 minutes at 10,000 x g at 4°C and discard the supernatant. There should be a gel-like white...Pro-Tip To prevent overdrying, watch the pellet and carefully remove any residual ethanol wash and add RNase-free... the bottom layer will be a red-pink color. Take care to not disturb or collect the interphase layer with...

  16. AAV Production in HEK293 Cells

    Type
    Protocol
    ...attached to the dish surface and should be handled carefully. Avoid touching the cells when replacing media...pyruvate, Corning 10-014-CV (optional) 7.5% sodium bicarbonate, Corning 25-035-CI (optional) 1 M HEPES, HyClone... 7.5 mL 100X glutaGRO, 7.5 mL of 7.5% Sodium Bicarbonate, 7.5 mL 1 M HEPES to 750 mL DMEM + 1 g/L glucose...stored at 4 °C for up to 2 months. After 2 months, discard the tube and thaw a new working stock. 40% POLYETHYLENE...incubator and pour the media into a waste container. Carefully add the OptiMEM + DNA + PEI solution to the CS5...cells (not poured on them) and can be adjusted by carefully tilting the CS5 back and forth. Pro-Tip To help...other components, such as sorbitol and sodium bicarbonate, sorbitol has been found to increase viral titers...
Showing: 401 - 420 of 625 results