pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
Type
Blog Post
... OriP and induces amplification of the episomes concurrently with DNA amplification in the host cell. ...possibilities for the clinical and therapeutic applications of iPSCs. The generation of human iPSCs (hiPSCs...hiPSCs, reprogramming methods that do not involve modification of the original genome by integrating viral ...where each vector contains a viral origin of DNA replication, OriP and the EBNA-1 sequence that encodes for...are necessary and sufficient for retention and replication of the vectors in human cells [8]. The EBNA-1...cocktail. pCXWB-EBNA-1 lacks oriP and is unable to replicate in human cells, thus expressing additional EBNA...., Recent technological updates and clinical applications of induced pluripotent stem cells. Korean J ...