...are more than welcome in contributing to The Addictive Brain. A contribution can either be an article,...This post was contributed by guest blogger Chinmaya Sadangi, a postdoc at the University of Toronto. ...

... use the material in human subjects or clinical trials Acknowledgement in recipient’s future publications... benefits, we know that legal documents can get tricky. Here’s how Addgene helps: For Providers: One-time... FREE!) and we’ll take it from there. We will distribute your materials under the UBMTA terms, so you ...

...difficulty with NgAgo, but a few successes have trickled into the survey. In response to a bit of a Twitter...to make single base edits in DNA. The first, contributed by guest blogger Kristian Laursen from Cornell...mutated plasmid constructs. The second post was contributed by Addgene Scientist Mary Gearing and discusses...

...The following post was contributed by Daniel Bauer and Matthew Canver of Boston Children’s Hospital and...frameshift mutations. However these may require restriction fragment length polymorphism analysis, allele-specific... you to our guest bloggers! Daniel Bauer is a pediatric hematologist and principal investigator at Boston...

...Yeast. 1998 Jul;14(10):953-61. Note: Marcy Patrick contributed to the writing of this article....isolation of successful transformants. Addgene distributes several ready-to-use, modular plasmids, combining...

...amazing plasmid technologies contributed by our depositors, Addgene strives to make it easier for you to...among all the noise. Scientists have a variety of metrics they can use to evaluate the impact of their work...

...heavy restrictions on what I could and couldn’t do as an intern. I expected that these restrictions would...choose what gene to focus on. The gene choice had restrictions. It couldn’t be a Covid gene, a toxin, or an...

...Recycle plastic packaging Packaging is a huge contributor to the increase in plastic manufacturing, accounting...accept and to print a free shipping label. Recycle nitrile gloves Terracycle is a company that takes items... a box with the prepaid label and fill it with nitrile gloves, affix the prepaid label, and divert these...

...NEBCutter2: For finding restriction enzyme cut sites Webcutter 2.0: For finding restriction enzyme cut sites ... different types of cloning methods, such as restriction enzyme cloning, Gibson assembly, or In-Fusion...

... a repair template, using Gibson Assembly or restriction enzyme cloning. Streptomyces bacteria are more... presence of just a single organism, such as Clostridium difficile, could be targeted and preferentially...base editing in Streptomyces Some of the most industrially and clinically relevant bacterial species reside...CM, Ousterout DG (2020) In Vivo Targeting of Clostridioides difficile Using Phage-Delivered CRISPR-Cas3...

... This post was contributed by Magdalena Julkowska, a postdoctoral researcher at KAUST, Saudi Arabia. ...general, I think that this is an (e.g. great contribution to the community because of..." This first couple...on-line repository (like GitHub, Zenodo, Figshare, Triad) while the wet-lab resources, like constructs or...

... such as Stbl3s. ITR loss can be detected by restriction digest using enzymes, like SmaI, which have recognition...organism or tissue type-specific, which can help you restrict the expression of your transgene to a specific...result is a permanently flipped transgene. By restricting the availability of Cre, lox sites lets researchers...

...Minigenomes - A Safe Way to Study Dangerous Viruses Contributed by guest blogger Tessa Cressy from the labs of...leaving virulent components behind. Minigenomes are stripped down viral genomes containing only the sequences...fantastic depositors and guest writers. Your contributions make Addgene and the Addgene Blog useful resources...

...This post was contributed by guest bloggers Samantha Zyontz and Neil Thompson from the MIT Sloan School...thanks to Addgenies Pamela McA'Nulty and Zachary Tribbett for helping compile the data used in this work..., computing performance, and randomized control trials in innovation. Visit his website at: http://www.neil-t.com...

... with biorender.com. ITR structure  ITRs are symmetrical 145-nucleotide sequences that flank the ends ...identification of non-dominant ITR sequences.  Restriction digests with the enzymes SmaI and SrfI can also...deletions and point mutations will go undetected by restriction digest methods.    Resources and references...

...tuberculosis Dr. Patricia Sheen, Universidad Peruana Cayetano Heredia, Peru Patricia Sheen, Professor ...Biology Resources Learn about Global Plasmid Distribution ...

... research community. At Addgene, we’ve helped distribute many of these technologies in plasmid form and...libraries. Please note, the pooled libraries we distribute in lentivirus format only deliver gRNA and therefore...like to thank all those who are allowing us to distribute their CRISPR tools as ready-to-use virus and,...

...developed in terms of the collection, storage, and distribution of the plasmids and their ability to identify...plasmids so scientists aren’t bogged down with distributing them and spending more time on research. What...LLP, auditing insurance, finance and high-tech industries. He has earned both an MBA in finance and a B.S...

...,850 SARS-CoV-2 plasmids to help academic and industrial labs accelerate research and to help find a faster...citation to the plasmid page on our website. Contributing your results helps other researchers using these...high throughput platforms, Addgene and Ginkgo are striking a balance by modifying certain processes in both...

...This post was contributed by guest bloggers Joachim Goedhart and Marieke Mastop from the Section of Molecular...sample. Practical brightness A protein with a high intrinsic brightness that does not fold well or cannot be...explanation of the assay is depicted in figure 1. The strict correlation between the fluorescent protein of ...