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  1. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...10.1089/hgtb.2013.131 Lock, M., McGorray, S., Auricchio, A., Ayuso, E., Beecham, E. J., Blouin-Tavel, ... Bosch, F., Bose, M., Byrne, B. J., Caton, T., Chiorini, J. A., Chtarto, A., Clark, K. R., Conlon, T.,...

  2. Pooled Library Amplifications

    Type
    Blog Post
    ...decide what transformation efficiency you need to achieve. Most pooled library protocols aim for somewhere...weight of your library plasmids. As long as you achieve sufficient coverage from your transformation, any...

  3. PhD Applications After COVID

    Type
    Blog Post
    ...research that aligns with your interests and goals.  Reaching Out to Potential PIs Even if you’re applying to...you determine if the program is the right fit. Reaching out to PIs also means doing plenty of mini-interviews...

  9. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...scientific training. Cartoons may be read as “for children” or may need text for audience interpretation....experience.  Can combines cons of multiple medias. Switching from one media type to another is cognitively ...

  10. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...limited in the level of resolution that they can achieve. This means that regardless of how powerful of ...evenly in all directions, aka isotropically. But achieving isotropic expansion depends on how well the gel...

  11. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...opportunities for his students. But he also found that his teaching responsibilities left him with limited time for...the U.S. Army, and hockey coach all shaped his teaching style, with the former offering excellent training...

  12. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...RNA that bind to a cell’s DNA, telling the Cas machinery where to cut.      Fig. 2: Heatmap indicating...when interpreting findings, particularly when researching ancestry groups. And, of course, it’s imperative...

  13. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...using a plasmid such as pET-28b-Cas9-His from Alex Schier’s Lab. gRNAs can be in vitro transcribed from ssDNA...publicly available by both the Jacob Corn and Alex Schier laboratories. Delivering Cas9-gRNA ribonucleoproteins...

  15. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...gene fusions for analyzing gene expression in Escherichia coli and yeast. Methods Enzymol. 1983;100:293...2547163. 5. Ozawa, T., Kaihara, A., Sato, M., Tachihara, K., Umezawa, Y.l. Split luciferase as an optical...

  17. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ... yourself, “I’m not married,” or “I don’t have children, so my life will be under control.” Think again...starve while you are away a few days. Also, your children will enjoy day care and school if you tell them...

  18. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ... a virus and the plant's post-transcriptional machinery to silence genes of interest (Unver and Budak,...plants." Plant Biology 5.02 (2003): 103-115. Hu, Ching-yeh, et al. "Intrinsic GUS-like activities in seed...

  20. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...efficient base rewriting in the genome became achievable in 2016 when two independent groups (Komor et...is either repaired by cellular mismatch repair machinery making an original C-G pair converted to T-A or...
Showing: 381 - 400 of 646 results