...Equipment needed: Mini PCR machines by DIY-Bio, P51 cardboard fluorescence visualizer with 9V battery Approach...fluorescently labelled RNA, the authors employ a P51 cardboard fluorescence visualizer that is powered by a 9V...

...issues Reproductive health Immigration Pollution Healthcare Science education To make scientists relatable...Scientists' Views PolitiFact: EPA head Scott Pruitt says carbon dioxide is not 'primary contributor' to global ...

...coupled with your technical skills, can open new career opportunities for you, if you can identify them...eye out for the phrase “I assumed they were taking care of that”, because that can indicate confusion around...

...First, CRISPR would need to be delivered to both cardiac and skeletal muscle cells, where precision editing... each observed the same positive phenotypes in cardiac and skeletal muscle. Even when the genome editing...

...cassette minimizes the sequence added, leaving a 34 bp “scar”, but currently requires additional experimental...efficiencies ranging from 11-100% of F1 animals carrying precise knock-ins, and homozygous knock-in animals...

...intrinsic selectivity of the ligase active site and careful balance of reaction conditions to reduce the incidence...Probes), use of a high fidelity ligase, along with careful probe design and reaction condition optimization...

...educational science led her to pursue a teaching career, and she now is a very happy scientific communication...Samulski. "Delivering transgenic DNA exceeding the carrying capacity of AAV vectors." Gene Therapy for Neurological...

...detergents such as triton-x, saponin, or tween. Take care when using strong detergents like triton-x and tween...while a droplet containing a negative cell is discarded. FACS can be performed aseptically allowing users...

...limitations or that other technologies need to be discarded but clearly we are in an era of neuroscience research... as the vector core at the University of North Carolina) that offer his constructs. Importantly, control...

...parts of the Cas9 enzyme.   Figure 1: A cartoon depiction of Cas9’s two major lobes, REC and NUC...process from start to finish:   Figure 9: Cartoon summary of Cas9 activity. The apo enzyme is shown...

... PubMed Central PMCID: PMC5529245. Choi, V.W., McCarty, D.M., and Samulski, R.J. (2005). AAV Hybrid Serotypes... and Holzbaur, E.L.F. (2014). Axonal Transport: Cargo-Specific Mechanisms of Motility and Regulation. ...

...selection curve below. Carbenicillin is used here in place of ampicillin because carbenicillin is more stable,...concentrations of carbenicillin plated over-agar. Control Plate with No Carbenicillin Plate shows a lawn...Plating of Carbenicillin. The above graph displays the stock concentration of Carbenicillin stock used...protocol will focus specifically on selection with carbenicillin; however it can be adapted for any antibiotic...antibiotic High concentration (100 mg/mL, 1000x) carbenicillin stock solution in sterile water (or other antibiotic...coli transformed with a plasmid containing the carbenicillin (amp) resistance gene (or other antibiotic resistance... resistance) Procedure Day 1 Prepare carbenicillin to a concentration of 1 mg/mL – 4 mg/mL in LB medium...

...you through Addgene's online resources. Career Videos Get career insight and advice from real Addgenies...discusses her career in science, the importance of international collaboration, and offers career advice for...how-to screen captures, lab procedure protocols, and career advice videos. Educational...protocols, tips on using Addgene's resources, and career advice. For written lab protocol material, visit...depositing plasmids with Addgene Depositing Page Career Videos (Link opens in a new window) Video Link ..., PhD In this first installment of the Addgene Careers series, we sit down with Addgene Outreach Scientist...Scientist Jessica Welch to discuss her career path, differences in research culture between the US and Australia...

...introduction for those who are just starting their careers in the lab and even those who might just need a...

...are currently three Gq DREADDs based on human muscarinic receptors: hM1Dq, hM3Dq, and hM5Dq, however, ...There are currently two Gi DREADDs based on human muscarinic receptors, hM2Di and hM4Di, and a Gi DREADD based...erythrocyte β-adrenergic receptor with a rat M3 muscarinic receptor. This DREADD was shown to have a small...DREADD, termed Rq(R165L), is based on the human M3 muscarinic receptor and has not been used in vivo . Figure...the text or table 1 for details. DREADD Ligands Muscarinic-receptor based DREADDs were engineered to respond... all been explored as alternative ligands for muscarinic-receptor based DREADDs. Compound 21 has similar...FDA and EMA approved. One pitfall of a suite of muscarinic-based DREADDs that all respond to the same ligands...

... de nuestra colección de libros electrónicos. Descargar CRISPR 101: Una herramienta de edición Addgene...

... again this year! All winners receive an Addgene care package with Addgene mugs and some delicious hot...

...°C and discard the flow through. Add your sample. Spin at 3500 rpm for 5–8 min at 4 °C, discard the flow...syringe and a blunt edge 18 ga Hamilton needle, taking care to avoid bubbles (Figure 1). 8 mL of 15% iodixanol...40% iodixanol step 3 mL of 60% iodixanol step Carefully add up to 5 mL of clarified supernatant on top...alternatively centrifuge for 2 h at 200,000 x g at 18 °C. Carefully take the QuickSeal tubes out of the rotor and ...elutes from the gradients. In a biosafety cabinet, carefully puncture the QuickSeal tube at the interface of...collected corresponds to the 60% phase and can be discarded. The fractions obtained from the 40% phase contain...the 40% step. Collect up to 5 mL per tube, taking care to avoid collecting the proteinaceous material at...

...Summer Internship Matteo Tardelli, who added to our career advice collection. Embracing Serendipity Nicolas...

... know? Fill out our referral form and we’ll take care of the rest. Know of a useful plasmid that’s not...