...mental-mistakes-that-kill-your-productivity Finkelstein, S. (2019, July 15). A brief exercise to spur innovation...

...many closely-packed labeled cells. Plus, Voltron2’s fluorescence is tunable by changing the dye that becomes...

...Foronda M, Zimmerman J, Diaz B, Zafra MP, Goswami S, Dow LE (2020) GO: a functional reporter system to...

...2958.2005.04643.x Pubmed PMID: 15916596 Kamruzzaman M, Shoma S, Thomas CM, Partridge SR, Iredell JR (2017) Plasmid...

...Many thanks to our guest blogger Gary McDowell! Gary S. McDowell is currently the Executive Director of Future...

...minor extension can usually ensure that the 3’-base(s) do not form secondary structures. The introduction...

... tactic, and puts you ahead of most start-up P.I.’s. It doesn’t have to be complicated, or difficult to...

...PMID: 25895059 Barnea, et al. Proc Natl Acad Sci U S A. 2008. PubMed PMID: 18165312 CIDAR MoClo parts ...or clusters. Veggiani, et al. Proc Natl Acad Sci U S A. 2016. PubMed PMID: 26787909 EasyClone 2.0 Yeast...Hanson SM, Rodríguez-Laureano L, Albanese SK, Gradia S, Jeans C, Seeliger MA, Levinson NM, Chodera JD. bioRxiv...

...24700457. PubMed Central PMCID: PMC4077948.  Yan, Kelley S., et al. "The intestinal stem cell markers Bmi1 and...

...antibiotics! References Espah Borujeni, Amin, Anirudh S. Channarasappa, and Howard M. Salis. "Translation ...

...Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR/Cas9-Derived Mutations...

...: 19363495. 2. Wang JW, Wang A, Li K, Wang B, Jin S, Reiser M, Lockey RF. CRISPR/Cas9 nuclease cleavage...

... approachable relationship for the mentee, where (s)he feels truly understood and supported, and can more...

...Common gene mutations found in E.coli strains Gene(s) Description Functional Consequence dam DNA adenine...

...endogenous gene(s) without permanently modifying the genome dCas9-activator (such as dCas9-VP64) gRNA(s) targeting... (CRISPRi) Reduce expression of a particular gene(s) without permanently modifying the genome dCas9-repressor...repressor (such as dCas9-KRAB) or dCas9 gRNA(s) targeting promoter elements of target gene dCas9-KRAB ...

...selective expression in the brain. Proc Natl Acad Sci U S A . 2010 Sep 21;107(38):16589-94. PubMed PMID 20807748...

...plasmid. Additionally, the restriction enzyme site(s) allow for the cloning of a fragment of DNA to be ...information and a more extensive strain list. Strain Vendor(s) Genotype BL21 Invitrogen; New England BioLabs E. ...datasheet or the plasmid map to confirm which antibiotic(s) to add to your LB media or LB agar plates. Antibiotic..., or T K G or T M A or C N A, T, C, or G R A or G S C or G V A, C, or G W A or T Y C or T Amino Acids ..., UUC Proline Pro P CCU, CCC, CCA, CCG Serine Ser S UCU, UCC, UCA, UCG, AGU,AGC Threonine Thr T ACU, ACC...SV40 NLS PKKKRKV or PKKKRKVG Protein C EDQVDPRLIDGK S Tag KETAAAKFERQHMDS SB1 PRPSNKRLQQ Webpage and Blog...

...1:24 - 29:31 - Reproducibility Overview - Jeffrey S. Flier, Researcher at Harvard Medical School, former...

...27786307. PubMed Central PMCID: PMC5476805. 8. Inagaki, S., et al. (2017) Genetically encoded bioluminescent...

...do in the future. Questions to ask: Will someone(s) train you technically? Will the PI/supervisor look...