...mental-mistakes-that-kill-your-productivity Finkelstein, S. (2019, July 15). A brief exercise to spur innovation...

...Foronda M, Zimmerman J, Diaz B, Zafra MP, Goswami S, Dow LE (2020) GO: a functional reporter system to...

...Many thanks to our guest blogger Gary McDowell! Gary S. McDowell is currently the Executive Director of Future...

...minor extension can usually ensure that the 3’-base(s) do not form secondary structures. The introduction...

...PMID: 25895059 Barnea, et al. Proc Natl Acad Sci U S A. 2008. PubMed PMID: 18165312 CIDAR MoClo parts ...or clusters. Veggiani, et al. Proc Natl Acad Sci U S A. 2016. PubMed PMID: 26787909 EasyClone 2.0 Yeast...Hanson SM, Rodríguez-Laureano L, Albanese SK, Gradia S, Jeans C, Seeliger MA, Levinson NM, Chodera JD. bioRxiv...

...antibiotics! References Espah Borujeni, Amin, Anirudh S. Channarasappa, and Howard M. Salis. "Translation ...

...: 19363495. 2. Wang JW, Wang A, Li K, Wang B, Jin S, Reiser M, Lockey RF. CRISPR/Cas9 nuclease cleavage...

... approachable relationship for the mentee, where (s)he feels truly understood and supported, and can more...

...Common gene mutations found in E.coli strains Gene(s) Description Functional Consequence dam DNA adenine...

...24700457. PubMed Central PMCID: PMC4077948.  Yan, Kelley S., et al. "The intestinal stem cell markers Bmi1 and...

...Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR/Cas9-Derived Mutations...

...selective expression in the brain. Proc Natl Acad Sci U S A . 2010 Sep 21;107(38):16589-94. PubMed PMID 20807748...

...of AAV serotypes, indicating the optimal serotype(s) for transduction of a given organ. Tissue Optimal... OS, Velardo MJ, Peden CS, Williams P, Zolotukhin S, Reier PJ, Mandel RJ, Muzyczka N. Mol. Ther. 2004 ...

...self-inactivating” (SIN) after integration. Packaging plasmid(s) Envelope plasmid Addgene’s packaging and envelope...one transfer plasmid one or two packaging plasmid(s) one envelope plasmid After media change and a brief... Retroviral integration site selection. Desfarges S, Ciuffi A. Viruses. 2010. Jan;2(1):111-30. PubMed ...

... Kang SH, Bae YJ, Hong JT, Chung YB, Lee CK, Song S (2006) PCR-based detection of mycoplasma species. ...

...27786307. PubMed Central PMCID: PMC5476805. 8. Inagaki, S., et al. (2017) Genetically encoded bioluminescent...

...do in the future. Questions to ask: Will someone(s) train you technically? Will the PI/supervisor look...

...1:24 - 29:31 - Reproducibility Overview - Jeffrey S. Flier, Researcher at Harvard Medical School, former...

...CRISPR Class 2 C lustered R egularly I nterspaced S hort P alindromic R epeat (CRISPR) systems, which ...

...Being a scientist in my late 20’s, new graduates often ask me for advice on careers available to new ...