...packaged into phage and then extracted. Besides taking a couple days to generate the phage-derived uracil...with mutational scanning: ligand binding assays, phage display assays, ubiquitination activity assays, ...template is generated by transforming a backbone phagemid vector into an E. coli strain that is deficient...

...transcription of the phage repressor cI. Repressor cI inhibits transcription from phage promoter pR, preventing...

...specificity landscape of the SH3 proteins using peptide-phage display and deep sequencing, revealing that they...small molecule binding screens, peptide arrays, and phage display selections. In addition to these constructs...

... activate programmed cell death or dormancy for phage-infected bacterial cells so as to limit the spread...

...aTc-inducible pTet-dCas9 in the attachment site of the phage 186. Detailed information about the repression system...

...editors By Susanna Stroik The Liu lab recently used phage-assisted continuous evolution and protein engineering... here!  Doman, J.L., Pandey, S., et al. (2023). Phage-assisted evolution and protein engineering yield...

...only store a record of invading phages but also to destroy the phages upon re-exposure. More specifically... VI (Cas13) Fighting Back: Anti-CRISPR Genes in Phage The CRISPR adaptive immune system seems like a winning..., but their function as a safeguard against bacteriophages was not elucidated until 2007. Scientists hypothesized...identified the first set of anti-CRISPR genes in bacteriophages infecting Pseudomonas aeruginosa . Anti-CRISPR...J, Pawluk A, Maxwell KL, Davidson AR. 2013. Bacteriophage genes that inactivate the CRISPR/Cas bacterial...

... reporter vector expressing firefly luciferase. pHAGE-CMV-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter...

...CRISPRi screens to identify essential genes and phage host factors, and identify sequence-specific toxicity...

...cells were transduced with a range of dilutions of pHAGE-TO-dCas9-3XmCherry . 72 h post transduction, cells...

...able to destroy DNA viruses, similar to the anti-phage CRISPR effect observed in bacteria. Learn more about...

...endo-lysosomal trafficking of adeno-associated virus/phage." Journal of Biological Chemistry (2012): jbc-M112...

... activate programmed cell death or dormancy for phage-infected bacterial cells so as to limit the spread...

... to maximize the mutation rate. For example the Phage Assisted Continuous Evolution (PACE) method requires...of the thioredoxin-binding domain (TBD) from bacteriophage T7 DNA polymerase increased the processivity...

...single array to target the genome of the invading phage pathogen. Over the past decade, CRISPR tools have...

... sequence if not methylated (if it comes from a phage for instance). Aside from restriction modification...

...and specificity. In this first, the Liu lab used phage-assisted evolution selection systems to generate... LW, Zeng J, Bauer DE, Doudna JA, Liu DR (2020) Phage-assisted evolution of an adenine base editor with... indel formation 1.5-2 fold, the team fused bacteriophage protein Gam to the N-terminus of BE4 (Komor ...Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors...

...challenge, the Uppalapati and Woolley lab developed a phage display technique using small scaffold proteins ...

...occur naturally in any known genome other than P1 phage, and is long enough that there is virtually no chance...from the P1 bacteriophage: the Cre recombinase and a loxP recognition site. The P1 bacteriophage uses these...Let us know in the comments! References 1. Bacteriophage P1 site-specific recombination. I. Recombination...in mammalian cells by the Cre recombinase of bacteriophage P1. Sauer, B. and Henderson, N. 1988. PubMed...

...μL total per 10 cm dish). Using transfer plasmid pHAGE TRE dCas9-KRAB (total μg of plasmid DNA 27.8 μg)...