...Articles Addgene's Fluorescent Protein Guide Note: A. Max Juchheim contributed to the writing of this article...

... and we would be happy to answer them! Note: A. Max Juchheim, Marcy Patrick, and Meghan Rego contributed...the 3rd-generation packaging systems offer the maximal biosafety available for the technology. The minor...

...described by researchers at the NIH here.  Note: A. Max Juchheim contributed to the writing of this article...

... using a chemical transfection reagent (e.g. PEI-MAX). Simply cotransfect a mixture of 1248 ng of CMV-...

...chloroquine. After the incubation, add the DNA:PEI-Max mix to the conical containing the media and chloroquine...

...65 mm Petri dish (VWR, 11019-552) 4 MaxiPreps (Qiagen HiSpeed Max, Catalog #12663) Tips (1000 µL, 200 ... for the purpose of a Maxiprep. Purify plasmid DNA using the Qiagen HiSpeed Maxi Kit (one conical is its...representation is maintained. Maxipreps - Less is more: Do not overload the Maxipreps as yield can dramatically...spread out the harvested cells further than four Maxipreps worth. Count colonies on the most dilute Petri... its own Maxiprep). Critical Do not freeze pellets for later purification. Immediately purify them! Commercial...Commercial Maxipreps rely on incremental, ordered cell lysis. E. coli cells are subject to lysis by freeze-thaw...Glycerol or DMSO solutions. Quantify the individual Maxipreps by Nanodrop, Picogreen, or Qubit. Each different...

...Suppmann, the Recombinant Protein Production group at Max-Planck Institute of Biochemistry has developed a ...Multisite Gateway compatible destination vector for a maximum of 24 elements assembled into a final transgenesis...

...interaction strengths. Erich Wanker’s group at the Max-Delbrueck Center for Molecular Medicine has recently...independent gRNAs, each chosen strategically to maximize the likelihood of creating a functional knock-...

...dCas9VP64 on pmax expression vector. 48224 pAC92-pmax-dCas9VP96 : dCas9VP96 on pmax expression vector. 48225... 48225 pAC93-pmax-dCas9VP160 : dCas9VP160 on pmax expression vector. 48226 pAC94-pmax-dCas9VP160-2A-puro...promoters. Table 2. pmax dCas9 Activator Expression Plasmids ID Plasmid 48223 pAC91-pmax-dCas9VP64 : dCas9VP64...Plasmid 48222 pAC90-pmax-DEST : Gateway Destination vector derived from Clontech's pmax (CAGGS) expression...activators and sgRNAs can be separately transfected (pmax dCas9 activators [48223~48227] + sgRNA expression...transferred to Gateway destination vectors (e.g., pmax-DEST [48222] and (Link opens in a new window) Lifetechnologies...2A-puro : dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Table 3. dCas9 Activators Gateway...

...Mouse IgG2a 114541 Anti-KCNU1/Slo3 maxi-K+ channel [N2/16R] KCNU1/Slo3 maxi-K+ channel Mouse Mouse IgG2a 114542...IgG2a 128634 Anti-Slo1/BKAlpha maxi-K+ channel [L6/23R] Slo1/BKAlpha maxi-K+ channel Mouse Mouse IgG2a ...IgG2a 177486 Anti-Slo1/BKAlpha maxi-K+ channel [L6/60R] Slo1/BKAlpha maxi-K+ channel Mouse Mouse IgG2a ...IgG2b 199406 Anti-Slo1/BKAlpha maxi-K+ channel [L6/60R-2b] Slo1/BKAlpha maxi-K+ channel Rat Mouse IgG2b 199407...IgG2a 206523 Anti-Slo1/BKAlpha maxi-K+ channel [L6/48R] Slo1/BKAlpha maxi-K+ channel Mouse Mouse IgG2a ...IgG2a 222180 Slo1/BKAlpha maxi-K+ channel [L6/60R-rat IgG2a] Slo1/BKAlpha maxi-K+ channel Mouse Chimera...Mouse 199421 Slo1/BKAlpha maxi-K+ channel scFv [L6/60] L6/60 scFv Slo1/BKAlpha maxi-K+ channel Rat Mouse 199422...

...indel frequency. Koblan et al., 2018 BE4max, ArcBe4max, ABEmax   Improved nuclear localization and expression...localization signals and codon usage BE4 to create BE4max and AncBE4max with a 4.2-6-fold improvement in editing...adenine base editing When the Liu lab created BE4max and AncBE4max mentioned above, they also made an adenine...., 2020 SpRY Cas9, available fused to ABEmax(7.10) or to BE4max   Evolved SpCas9 with NRN or NYN PAM site... more flexible sequence preferences, evoAPOBEC1-BE4max, which is significantly more effective at editing...localization and expression. This base editor was named ABEmax. In 2020, two papers were published describing ...programmable adenine and cytosine editor) by fusing miniABEmax-V82G and Target-AID to Cas9 (Grünewald et al....

... continued to improve the maximum efficiency of prime editing. While maximum efficiency isn’t everything... PE2-5 approaches, sometimes referred to as PE2max, PE3max, etc. PE6: Specialized prime editing variants...end of PEmax to produce PE7. PE7 significantly improves editing efficiencies compared to PEmax with normal... originally introduced, rely on the PE2 enzyme. PEmax: Optimizing prime editor sequence to improve expression...be combined with any of the PE2-PE5 strategies. PEmax contains a reverse transcriptase with a sequence... although both enzymes still approach or exceed PEmax editing efficiencies for short, simple edits. PE6c... Innovation Purpose PE2 PE3 PE4 PE5 PEmax PE6 PE7 Pentamutant reverse transcriptase...

...spectrum shows two maxima, one local maximum at just under 500 nm and a global maximum at around 560 nm,... global excitation maximum lies around 650 nm, APC-Cy7's global excitation maximum is about 750 nm. If...and emission spectra. Both spectra possess clear maxima where excitation and emission are the most efficient...fluorophores. Each fluorophore has a defined emission maximum. Note, you can also use the tool to display excitation...wavelength of 488 nm (Table 1), which is near a local maximum of PE's excitation spectrum (dotted line, Figure...excites at 561 nm, which is close to the global maximum of PE's excitation spectrum where the excitation...

... M, Rollins MF, Hidalgo-Reyes Y, Wiedenheft B, Maxwell KL, Davidson AR. 2015. Multiple mechanisms for ...136-9. PMID: 26416740 Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR. 2013. Bacteriophage genes that...Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR. 2016. Naturally occurring off-...

... a structured motif to enhance pegRNA stability PEmax - optimized PE architecture; increased synergy between.... PMID: 25079318 Bondy-Denomy, J., Pawluk, A., Maxwell, K. L., & Davidson, A. R. (2013). Bacteriophage...Rollins, M. F., Hidalgo-Reyes, Y., Wiedenheft, B., Maxwell, K. L., & Davidson, A. R. (2015). Multiple mechanisms...Kranzusch, P. J., Sontheimer, E. J., Davidson, A. R., Maxwell, K. L., & Doudna, J. A. (2017). A Broad-Spectrum..., J., Edraki, A., Shah, M., Sontheimer, E. J., Maxwell, K. L., & Davidson, A. R. (2016). Naturally occurring...

... 50% of its maximum value. Maturation is the time for fluorescence to obtain half-maximal value. Structure...Color) For each table: Excitation and Emission maximum are listed in nm. Brightness is the product of ...

...inhibit MMR PE5 - PE3 plus MLH1 mutant to inhibit MMR PEmax - optimized PE2 enzyme containing RT optimized for...RNA-binding exonuclease protection factor La to PEmax to enhance pegRNA stability epegRNA - additional...

...mulatta). Cas-Designer searches for targets that maximize knockout efficiency while having a a low probability...dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression: pAC91 , pAC92 , pAC93...

...mutations). Its excitation maximum is reported as 603 nm, while the emission maximum is 675 nm. mCarmine is...boundaries of FP technology by shifting the emission maxima toward the red end of the spectrum (above 600 nm...

...molecules have a specific wavelength that they maximally absorb. The spectrophotometer measures these absorbances...contaminants. Nucleic acids (DNA and RNA) absorb maximally at 260 nm. Proteins on the other hand absorb best...nm and organic compounds and chaotropic salts maximally absorb at 230 nm. The A260/A280 ratio is used ...