...Genetic Code Expansion You may also like... GCE4All: Making Genetic Code Expansion Accessible Using Phosphoserine...with multiple copies of the tRNA. Applications By making small changes in selected amino acids within a ...working off of a previously established protocol, make sure to match the growth medium, ncAA concentration...unallocated codon. Therefore many controls must be used to make sure that these conditions are true. Always express...protein with and without the ncAA in the media to make sure that the full length protein is only made when...

... vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Packaged on Request...protocols for how to use your viral prep and how to make your own preps. Recipient Instructions What to do...purified DNA from the original plasmid that was used to make the virus. In order for Addgene to provide viral...

... CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible Degron Tagging Masato Kanemaki's lab has developed a ...alternative DSB repair pathway, instead of HDR, which makes it easy to construct a donor vector, because MMEJ...

...reagents are appropriate for a given experiment. Make sure to check whether reagents are available to ...efficiency compared to NHEJ knockout. Base editors can make a limited set of mutations. Repress or Interfere...envelope plasmids provide the necessary components to make lentiviral particles (for details about lentivirus...

... stable, making time course experiments possible, but its short luminescence half-life makes it too dim...Renilla luciferase have short protein half-lives, making them useful as transcriptional reporters. However...

...expression systems. Addgene has partnered with Ginkgo to make these plasmids available to scientists in academic...Codon-optimized expression (recode) Truncated for making pseudovirus (Ctrunc) ID Plasmid Description Mutations...

...plasmids and backbones with high cloning potential. By making these plasmids available as cgDNA, we hope to aid...note that this does not guarantee that Addgene will make cloning grade DNA preps of this plasmid available...

...purified DNA from the original plasmid that was used to make the virus. For more details, see our FAQ on What... vast catalog of eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our ...

...engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML, Thibodeau-Beganny...

...plasmids. Animals - Antibodies produced in animals make use of the animal’s immune system. The antigen of...Overview of application Collect and lyse samples to make proteins available. Tissue samples may need additional...Ready to put your new knowledge to use? Addgene now makes ready-to-use recombinant antibodies made from plasmids...

...finger nucleases (ZFNs) are effective tools for making targeted mutations in zebrafish. With support ...engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML et al....

...perform comprehensive quality control processes to make sure it is ready for your experiments. Shipping ...experience, from methods to troubleshooting insights, you make research more reproducible for scientists everywhere...

... 18 TAL effector repeats. The kit can be used to make arrays from 12-31 repeats. Perhaps one of the most...TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt...

... Selectable Marker PI Publication Epigenetics To make targeted epigenetic modifications, researchers have... require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system. ID Plasmid Gene...

...of target sites that flox your genes of interest makes FLEx switch a powerful experimental tool. For more...interest by removing neoR and stop sequence; used for making transgenic mice. Plasmid 15037 also contains IRES-EGFP...transfection efficiency, selecting desired clones, and making transgenic mice. ID Plasmid/Article Description...

...and improving the orthogonal translation system to make singly or multiply phosphorylated proteins (Pirman...expressing recombinant phosphoproteins. These attributes make rEcoli XpS ( 192872 ) and pSerOTS-C1* ( 188537 )...

...Targeting vector for the mouse Rosa26 locus; Used to make Cas9 knockin mouse Vectors for cancer modeling 60224...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. Nature . 2015...

... secreted, (semaphorin) 3E KIAA0331, M-SEMAH, M-SemaK, SEMAH, coll-5 SEMA3F sema domain, immunoglobulin...domain, secreted, (semaphorin) 3F SEMA-IV, SEMA4, SEMAK SEMA3G sema domain, immunoglobulin domain (Ig), ...H-SEMA-K1, H-Sema-L, JMH, MGC126692, MGC126696, SEMAK1, SEMAL SLIT1 slit homolog 1 (Drosophila) MEGF4,...

...you’ll likely need to tag it with something that makes it easy to see or easy to blot. The plasmids in...Christopher Voigt When combined with pCph8 and pPLPCB(S), makes lacZ expression inducible by red light. pCph8 50552...

... gRNA sequence in your particular experiment, so make sure to check out the particular conditions of the...GGGGCCACTAGGGACAGGAT 72833 cut S. pyogenes 27052166 Kanemaki mmp21 D. rerio CGGAGCTGATCACTGACA 72890 cut S....