...plasmids and backbones with high cloning potential. By making these plasmids available as cgDNA, we hope to aid...note that this does not guarantee that Addgene will make cloning grade DNA preps of this plasmid available...

...engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML, Thibodeau-Beganny...

...expression systems. Addgene has partnered with Ginkgo to make these plasmids available to scientists in academic... Truncated SARS-CoV-2 Spike gene constructs for making VSV pseudovirus Common human coronavirus (OC43,...

... I found Vectr to be fairly intuitive and I was making figures quickly. Inkscape Inkscape is the most...🔬! We built this so you could stop struggling to make figures in PPT 🔬 Also, the base version is free...

...scientists ask me about mentoring. Here they are: What makes a good Mentor? How do I choose an Advisor/PI who... benefits of taking on my own Mentee? Q1. What makes a good science mentor? I started a conversation ...their experience. This led to the topic of “What makes a good Mentor?” Some of the Tweeted comments: “...good Jedi does not necessarily an excellent mentor make. Are your current mentors/advisors like Yoda? If...for you) Helps identify a Mentee’s strengths and make the most of them Is not threatened by the Mentee...

...finger nucleases (ZFNs) are effective tools for making targeted mutations in zebrafish. With support ...engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML et al....

...plasmids. Animals - Antibodies produced in animals make use of the animal’s immune system. The antigen of...Overview of application Collect and lyse samples to make proteins available. Tissue samples may need additional...Ready to put your new knowledge to use? Addgene now makes ready-to-use recombinant antibodies made from plasmids...

...perform comprehensive quality control processes to make sure it is ready for your experiments. Shipping ...experience, from methods to troubleshooting insights, you make research more reproducible for scientists everywhere...

... 18 TAL effector repeats. The kit can be used to make arrays from 12-31 repeats. Perhaps one of the most...TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt...

... Selectable Marker PI Publication Epigenetics To make targeted epigenetic modifications, researchers have... require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system. Plasmid Gene...

...studied PIPs. The straightforward nature of BiFC also makes it easier to generate a large number of constructs...Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection. Cellular expression Whether...localization may differ from those of your BiFC construct, making it difficult to interpret your results. Unfortunately...cells. The ease with which BiFC can be visualized makes it amenable to studying PPIs under relatively physiological...

...individual features that make each tool unique. The CRISPR Software Matchmaker is composed of these features... the end of 2014, I began developing software to make sgRNA design accessible to all. At the time I thought...over another. On the other hand some software tools make use of empirically derived and/or prior knowledge... and parameters. This tapestry of ideas is what makes choosing the right tool confusing and it means that...at the glossary section of the CRISPR Software Matchmaker, it provides a list of terms I think are important...going shopping for one. Use the CRISPR Software Matchmaker to select the best tool based on your needs. ... and cherry pick the features that will actually make a difference in our experiments and lives. To do...

...and improving the orthogonal translation system to make singly or multiply phosphorylated proteins (Pirman...expressing recombinant phosphoproteins. These attributes make rEcoli XpS ( 192872 ) and pSerOTS-C1* ( 188537 )...

...of target sites that flox your genes of interest makes FLEx switch a powerful experimental tool. For more...interest by removing neoR and stop sequence; used for making transgenic mice. Plasmid 15037 also contains IRES-EGFP...transfection efficiency, selecting desired clones, and making transgenic mice. ID Plasmid/Article Description...

...Targeting vector for the mouse Rosa26 locus; Used to make Cas9 knockin mouse Vectors for cancer modeling 60224...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. Nature . 2015...

...you’ll likely need to tag it with something that makes it easy to see or easy to blot. The plasmids in...Christopher Voigt When combined with pCph8 and pPLPCB(S), makes lacZ expression inducible by red light. pCph8 50552...

... secreted, (semaphorin) 3E KIAA0331, M-SEMAH, M-SemaK, SEMAH, coll-5 SEMA3F sema domain, immunoglobulin...domain, secreted, (semaphorin) 3F SEMA-IV, SEMA4, SEMAK SEMA3G sema domain, immunoglobulin domain (Ig), ...H-SEMA-K1, H-Sema-L, JMH, MGC126692, MGC126696, SEMAK1, SEMAL SLIT1 slit homolog 1 (Drosophila) MEGF4,...

... gRNA sequence in your particular experiment, so make sure to check out the particular conditions of the...GGGGCCACTAGGGACAGGAT 72833 cut S. pyogenes 27052166 Kanemaki mmp21 D. rerio CGGAGCTGATCACTGACA 72890 cut S....

...transparency and accessibility in research and therefore makes the sequences of these affinity reagents available...