We narrowed to 166 results for: ipp
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TypeBlog Post...which is limited to the hippocampus. Thus, only neurons signaling from the hippocampus to Broca’s area would...access all the neurons that signaled from the hippocampus to Broca’s area. This could be done by injecting...of a mouse specifically expressing Cre in the hippocampus (Fig. 3). The Cre-dependent AAV would access ...
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The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog Post...repository. 100,000 Plasmids Shipped! - By 2010, Addgene had hit our stride. We shipped our 100,000th plasmid... 10 years ago today. In that time, Addgene has shipped over 350,000 individual plasmids to 5,000 different... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog Post...this information in mind if you’re planning on shipping something on dry ice or if you’re receiving samples...for 48 hours (to replicate how our viruses are shipped), where they would be exposed to a closed environment...function optimally at -80°C. For samples that are shipped on dry ice, this can affect the pH of the sample... -
Technical Design of a Western Blot
TypeBlog Post...nitrocellulose. PVDF is a sturdy membrane that can be stripped and reprobed multiple times. It doesn’t degrade...washes. It’s less sturdy and not recommended for stripping and reprobing, unless you buy specialty nitrocellulose...nitrocellulose membranes specifically developed to allow stripping. And as a safety note, nitrocellulose is flammable...enzymes, they are more likely to be compatible with stripping and probing. Imaging At the final step of your... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog Post...expertise in shipping hundreds of plasmids a day, the Lab and Office Teams develop shipping systems and...provide extensive logistics support for export and shipping on dry ice. The Business and Finance Teams continue... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog Post...manipulate RNA species through exon skipping. Previous CRISPR exon-skipping approaches used two guide RNAs ...iPS-derived cortical neurons, dCasRx-mediated exon skipping improved the ratio of pathogenic to non-pathogenic... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...studies have found reduced BDNF levels within the hippocampus and frontal cortex during depression, yet there...injecting lentiviral vectors expressing BDNF in the hippocampus causes a shift towards antidepressant behavior...-mediated suppression of microRNA124a in the hippocampus evokes antidepressants-like effects in rats."... -
An Inside Look into Addgene's Dev Team
TypeBlog Post...could have a shipping sprint and all the stories will touch different parts of the shipping code. The following...that the checkout takes the user directly to the shipping step, and no longer takes the user to a terms ... -
Filming Science Videos in the Age of Social Distancing
TypeBlog Post...using a simple, external video recorder and a mic clipped to her shirt. She rolled several long takes, and... videos like this would prove to be difficult. Shipping the gear, and walking everyone through the recording...record into an external recorder using a lav mic, clipped to the talent’s shirt. But knowing I would have... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog Post...by chemical treatment or photobleaching or by stripping out the antibodies, then start over with the next... β1 (white) and anti-GABA-AR β3 (green). (C) Hippocampus labeled with anti-Kv2.1 (magenta), anti-AnkyrinG...that your target protein is expressed in the hippocampus, or counting the number of puncta in each cell... -
DNA Service - Cloning Grade DNA
TypeCollection...including the check out and will require separate shipping charges. Where can I find cloning resources or... -
E11 Bio PRISM Collection
TypeCollection...neurons across 10 million cubic microns of mouse hippocampus using PRISM. Adapted from Park & Sheridan et ... -
Viral Vector Guides and Plasmids
TypeCollection...viral vectors, wild-type viral genomes have been stripped of any unnecessary accessory genes and divided... -
Twenty Years of Sharing Science!
TypeBlog Post...countries. Just eight days into 2024, we've already shipped 2,078 items to 764 scientists at 375 organizations...different countries. It's safe to say we keep our shipping team busy! But they're not alone: to better serve... -
mTOR Pathway
TypeCollection... B1 TSC1/2 TSC1 TSC2 Tuberous sclerosis VHL Von Hippel-Lindau tumor suppressor Return to top mTORC2 Complex... -
Distribution to Industry
TypeCollection...MTA documents, your order will be processed and shipped. Industry Distribution Policy A portion of Addgene's... -
New Viral Vectors - Spring 2025
TypeBlog Post...allow scientists to conduct experiments faster, skipping the preparation steps of production, purification... to the convenience of smaller sizes and quick shipping. We continue to expand the collection to meet ... -
MassBioEd Career Exploration Day at Addgene
TypeBlog Post...of busy Addgenies preparing plasmid samples for shipping. This was the favorite part of their visit for...sure to tell us what we already knew about our Shipping Operations Coordinator: “Kory was awesome!”. The... -
Promega Plasmid Collection
TypeCollection...Orders of the 20 µg DNA size are processed and shipped within 6–8 business days. For more information ... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog Post... Shah NG, McKenzie A 3rd, Steever AB, Wach A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):943-51....3rd, Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):953-61....