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Showing: 21 - 40 of 149 results
  1. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...which is limited to the hippocampus. Thus, only neurons signaling from the hippocampus to Broca’s area would...access all the neurons that signaled from the hippocampus to Broca’s area. This could be done by injecting...of a mouse specifically expressing Cre in the hippocampus (Fig. 3). The Cre-dependent AAV would access ...
  2. mTOR Pathway

    Type
    Collection
    ... B1 TSC1/2 TSC1 TSC2 Tuberous sclerosis VHL Von Hippel-Lindau tumor suppressor Return to top mTORC2 Complex...
  3. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...this information in mind if you’re planning on shipping something on dry ice or if you’re receiving samples...for 48 hours (to replicate how our viruses are shipped), where they would be exposed to a closed environment...function optimally at -80°C. For samples that are shipped on dry ice, this can affect the pH of the sample...
  4. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...expertise in shipping hundreds of plasmids a day, the Lab and Office Teams develop shipping systems and...provide extensive logistics support for export and shipping on dry ice. The Business and Finance Teams continue...
  5. Technical Design of a Western Blot

    Type
    Blog Post
    ...nitrocellulose. PVDF is a sturdy membrane that can be stripped and reprobed multiple times. It doesn’t degrade...washes. It’s less sturdy and not recommended for stripping and reprobing, unless you buy specialty nitrocellulose...nitrocellulose membranes specifically developed to allow stripping. And as a safety note, nitrocellulose is flammable...enzymes, they are more likely to be compatible with stripping and probing.   Imaging At the final step of your...
  6. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...could have a shipping sprint and all the stories will touch different parts of the shipping code. The following...that the checkout takes the user directly to the shipping step, and no longer takes the user to a terms ...
  7. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...studies have found reduced BDNF levels within the hippocampus and frontal cortex during depression, yet there...injecting lentiviral vectors expressing BDNF in the hippocampus causes a shift towards antidepressant behavior...-mediated suppression of microRNA124a in the hippocampus evokes antidepressants-like effects in rats."...
  8. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...using a simple, external video recorder and a mic clipped to her shirt. She rolled several long takes, and... videos like this would prove to be difficult. Shipping the gear, and walking everyone through the recording...record into an external recorder using a lav mic, clipped to the talent’s shirt. But knowing I would have...
  9. COVID-19 Resources

    Type
    Collection
    ...transcriptase expression plasmids from the Drew Endy and Philippa Marrack Labs. Point-of-care testing for COVID-...
  10. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGAACGAGAGGAGTGTGGcacactatgccacatgCAACTCTCCTCCAGACAA ippk TAL3102 & TAL3103 TCCTCAACATTATGGACTatagcaaacatgtcatGAAGCCTTTGCTTGGGGA...
  11. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...by chemical treatment or photobleaching or by stripping out the antibodies, then start over with the next... β1 (white) and anti-GABA-AR β3 (green). (C) Hippocampus labeled with anti-Kv2.1 (magenta), anti-AnkyrinG...that your target protein is expressed in the hippocampus, or counting the number of puncta in each cell...
  12. Retrograde AAV viral preps

    Type
    Collection
    ... - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a Color-flipping switch. Expresses NLS-mCherry in the absence of...
  13. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...countries. Just eight days into 2024, we've already shipped 2,078 items to 764 scientists at 375 organizations...different countries. It's safe to say we keep our shipping team busy! But they're not alone: to better serve...
  14. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ...of busy Addgenies preparing plasmid samples for shipping. This was the favorite part of their visit for...sure to tell us what we already knew about our Shipping Operations Coordinator: “Kory was awesome!”. The...
  15. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ... Shah NG, McKenzie A 3rd, Steever AB, Wach A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):943-51....3rd, Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):953-61....
  16. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ... Their cooler boxes come with a prepaid return shipping label. To return the box, simply peel your address...details on what they accept and to print a free shipping label. Recycle nitrile gloves Terracycle is a ...
  17. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    ...coronavirus-related deposits. Our valiant lab and shipping staff agreed to come in and start getting plasmid...plasmid deposits into the collection and to start shipping. They created the best safety protocols they could...
Showing: 21 - 40 of 149 results