...RNA Polymerases Other Enzymes DNA Ligases Addgene ID Plasmid Gene/insert 165503 pOpen-HSlig4 DNA Ligase... Ligase III Return to top DNA Polymerases Addgene ID Plasmid Gene/insert 165500 pOpen-9N7polA 9 degrees...Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid Gene/insert 165504 pOpen-EcoRIR EcoRI 165506...NcoI Return to top Reverse Transcriptases Addgene ID Plasmid Gene/insert 165546 pOpen-MMLV_RT (mut H) ...Transcriptase Return to top RNA Polymerases Addgene ID Plasmid Gene/insert 165505 pOpen-T7gene1 T7 RNA Polymerase...RNA Polymerase Return to top Other Enzymes Addgene ID Plasmid Gene/insert 165509 pOpen-TEV protease TEV...consists of more than 75 genes for enzymes that are considered "workhorses" of molecular biology, including ...

...Use this guide to learn about the applications of fluorescent proteins and to find the plasmids that ... Special Collections Fluorescent Protein Guide Fluorescent Protein Plasmids & Resources You May... For Multi-Color Imaging Blog: Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Fluorescent...variety of fluorescent protein plasmids. Use this guide to learn more about the many applications of fluorescent...fluorescent biosensors. Optogenetics Plasmids | Guide Use light to detect, measure, and control molecular... actuators or sensors. Chemogenetics Plasmids | Guide Use small molecules to activate genetically engineered...Localization Determine where your protein of interest resides by using a well-characterized fluorescent fusion...

... and rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites and Mutations 55636 pAAV-EF1a-Cre...pAAV-CaMKIIa-Flpo None Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations 55641 pAAV-Ef1a-fDIO... Dual recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations 55650 pAAV-hSyn... NOT Cre Dual recombinase-dependent: GECI Addgene ID Plasmid Logic Sites and Mutations 137119 pAAV-EF1a-Con... recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations 55645 pAAV-hSyn... recombinase-dependent: Inhibitory Opsins Addgene ID Plasmid Logic Sites and Mutations 137148 pAAV-nEF-Con...NOT Cre Dual recombinase-dependent: DREADDs Addgene ID Plasmid Logic Sites and Mutations 177669 pAAV-nEF-Coff...

...yeast. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacteria ID Plasmid Gene...Insert Promoter Selectable Marker PI Publication Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI...Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying molecules associated... are more straightforward and enable direct identification of molecules associated with a genomic region...

.... Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID Plasmid Gene...Gene/Insert PI Publication Plant ID Plasmid Gene/Insert PI Publication Do you have suggestions for other...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...regardless of homology to the crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require...require that each DNA target have high sequence identity to the spacer sequence and contain a PAM sequence...degradation seen in bacteria. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna...

.... For more information, see our Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection...DSBs or cuts ) or single-stranded breaks ( nicks ). ID Name Insert Function Antibiotic Resistance Activity...transactivator and used as a transcriptional activator . ID Name Insert Antibiotic Resistance Activity PI 61422...HSF1-2A-Hygro-WPRE) Zhang Pooled Barcoding Libraries ID Name Description PI 115643 Morris CellTag Barcode...our repository that express fluorescent proteins. ID Name Insert Antibiotic Resistance Purpose PI 17446...sgRNA library Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting 19,114...sgRNA library Brie in lentiGuide-Puro Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637...

...opens in a new window) . Browse Available PHP.eB AAV ID Name Promoter Description Category PI Controls 28306...plasmid (Addgene #103006) . Browse Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-...plasmid (Addgene #127847) . Browse Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP... (Addgene #185136) . Browse Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-... (Addgene #185137) . Browse Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-... (Addgene #175004) . Browse Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-... (Addgene #175005) . Browse Available CAP-B22 AAV ID Name Promoter Description Category PI 37825 pAAV-...

...and plants. Mammalian ID Plasmid Gene/Insert Promoter PI Publication Bacteria ID Plasmid Gene/Insert Promoter... Promoter PI Publication Plant ID Plasmid Gene/Insert Promoter PI Publication Last reviewed on: January...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...

...mTORC1 Activity in Living Cells", "mitochondria"). ID Plasmid Description Gene/Insert Vector Type PI Publication...Arosio Chloride (Cl-) Dual ClopHensorN chloride and proton sensor A genetically-encoded chloride and pH...1295-7. Samuel Miller Chloride (Cl-) ClopHensor biosensor for intracellular chloride and pH Arosio Lab ClopHensor...(6):e99095. Joseph Santos-Sacchi Chloride (Cl-) ClopHensorN chloride sensor (cytosolic) Multiplexed Optical...Cohen Chloride (Cl-) Membrane-targeted biosensor for monitoring changes in intracellular chloride A sensitive...Okumoto Hydrogen Peroxide (H 2 O 2 ) Fluorescent reporter of cytosolic hydrogen peroxide Monitoring the ...Hydrogen Peroxide (H 2 O 2 ) HyPer biosensor to measure changes in intracellular hydrogen peroxide concentration...

...glycine- and serine-containing peptide linker (GGSGGS) repeat pET28CLY2 Peptide linker standard consisting...Collections Fluorescent Proteins FRET Fluorescent Protein Guide: FRET More Fluorescent Protein Resources: Empty ...proteins connected via 5 amino acid linkers pET28CLY1 Peptide linker standard consisting of ECFP and EYFP connected...and EYFP connected by 2 GGSGGS repeats pET28CLY3 Peptide linker standard consisting of ECFP and EYFP connected...connected by 3 GGSGGS repeats pET28CLY4 Peptide linker standard consisting of ECFP and EYFP connected by... by 4 GGSGGS repeats pET28CLY5 Peptide linker standard consisting of ECFP and EYFP connected by 5 GGSGGS...GGSGGS repeats pET28CLY6 Peptide linker standard consisting of ECFP and EYFP connected by 6 GGSGGS repeats...

...Collections Fluorescent Proteins In Vivo Imaging Guide Fluorescent Proteins: In Vivo Imaging More Fluorescent...photoactivatable fluorescent empty backbone plasmids are ideal for single or multicolor in vivo imaging. Plasmids...

...markers of general cellular organelles and structures. ID Plasmid Structure Targeting Gene/Signal Fluorescent...table below to find markers of cytoskeletal element. ID Plasmid Structure Targeting Gene/Signal Fluorescent...table below to find markers for cellular junctions. ID Plasmid Structure Targeting Gene/Signal Fluorescent... to find markers of secretory pathway components. ID Plasmid Structure Targeting Gene/Signal Fluorescent...encoding fluorescent proteins tagged with genes or peptides with known subcellular localization patterns. ...important to determine where your protein of interest resides. There are a variety of methods for determining...red fluorescent protein (see this fusion protein guide to find the appropriate backbone). Then, by examining...

...Collections Fluorescent Protein Guide Empty Backbones Fluorescent Protein Guide: Empty Backbones More Fluorescent... For Multi-Color Imaging Blog: Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Blog...product of exctinction coefficient and quantum yield divided by 1000. pKa is the pH at which fluorescence intensity...Yeast Thorn Lab Vectors - Includes tagging with a wide variety of colors, some specifically optimized for...

... Synthetic Biology SynBio Guide Synthetic Biology: Assembly Standards Guide SynBio Resources SynBio Home...after the promoter, and so on. The following table provides information for the assembly standards most commonly...standards, there are additional sites which should be avoided whenever possible. Other popular cloning strategies...MoClo . Check out our Molecular Cloning Techniques Guide for an overview of the most common methods. BioBrick...

...protein can be paired with a wide variety of Cas-containing plasmids. ID gRNA Plasmid Promoter Cloning... HDR occurs at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Activate...target the dCas9-activator to your specific locus. ID Plasmid Gene/Insert Promoter PI Publication Empty...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...

...proteins of interest. Browse or search the table below. ID Plasmid Gene/Insert Mutations Industry Return to ...deposited with Addgene. Browse or search the table below. ID Plasmid Description Gene/Insert Industry PI Return...

...Strains Bacterial strains for generating adenovirus. ID Strain Description PI 16398 BJ5183 Strain for recombination...for adenoviral transfer plasmids that have inserts. ID Plasmid Type Description PI 16400 pAdEasy-1 Adenoviral...genome that can be assembled in a one-tube reaction. ID Kit Description PI 1000000176 AdenoBuilder toolkit...Adenovirus Plasmids Additional Resources: Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids...generate adenovirus particles. Read our Adenoviral Guide for more information about: Adenoviral components...

...Lentiviral vectors for constitutive shRNA expression ID Plasmid Description PI 10878 pLKO.1 ‐ TRC cloning...vectors for conditional (Cre-lox) shRNA expression ID Plasmid Description PI 11578 pSico Cre addition causes...Lentiviral vectors for Tet-regulated shRNA expression ID Plasmid Description Tet on or off PI 11643 pLVCT-...Retroviral vectors for constitutive shRNA expression ID Plasmid Description PI 8452 pMKO.1 puro Retroviral...monitor expression; Designed for inducing RNAi in a wide range of cell types, tissues and organisms Luk Parijs...

...systems. Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Plant ID Plasmid Gene/Insert...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...local chromatin state. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...entries describing enzyme activity, experimental considerations, and more, created and maintained by the Doudna...