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  2. Lentivirus Plasmids

    Type
    Collection
    ... plasmid. Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with constitutive or inducible... Tet-based inducible shRNA or cDNA expression, gateway plasmid, constitutive Hygromycin resistance gene...of pSLIK. Fraser 15948 pLOVE 3rd Tet inducible gateway destination plasmid for cDNA expression Ramalho-Santos...PGK driven puromycin Sabatini 25895 pLX301 3rd Gateway plasmid for CMV driven expression of cDNA. See ...pCW57.1 3rd Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro. See article for more versions...

  4. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...methods (restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning method is ultimately...

  7. Microbiology Resources

    Type
    Collection
    ... - Borodina Lab Golden GATEway cloning system - Wittbrodt Lab MultiSite Gateway Kit : Creating three-part...oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors for expression, markers, fusion...

  8. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...repression), and Fok1-dCas9 (dimeric nuclease). Gateway assembly method Frew Lab Multiple Lentiviral Expression...should be compatible with type IIS enzyme BfuAI. Gateway cloning is then used to combine the multiple gRNAs...

  13. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...74334 pCR8-Magneto2.0-p2A-mCherry Gateway 74333 pCR8-Magneto2.0 Gateway 74307 pAAV-CMV-DIO-Magneto2.0...The collection is compatible with both standard Gateway cloning and the FNLCR Combinatorial Cloning Platform...mammalian systems. The DULIP system is comprised of Gateway-compatible plasmids in which “bait” proteins are...

  14. Tetracycline Inducible Expression

    Type
    Collection
    ...Lentiviral vector for inducible expression for Gateway cloning; selection cassette in format: PGK-rtTA...Doyon 58245 pGLTR-X-GFP Single vector lentiviral Gateway RNAi system for conditional cell line generation... Off Verma 26803 pEnt L1L3 EF1a-tTA-2 Contains Gateway L1L3 sites and EF1α promoter driving tTA; contains...

  15. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...of protein in bacterial cells: the pET, pRSET, Gateway pDEST, and pBAD vectors for example. Protein expression...

  16. Bacterial Expression Systems

    Type
    Collection
    ...required. pET-HIS-Sangamo 40786 TALEN Gang Bao Gateway cloning and expression of an N-terminally His-tagged...endonuclease domain. pET-Sangamo-His 40787 TALEN Gang Bao Gateway cloning and expression of a C-terminally His-tagged...N-terminal 6x His tag and an MBP tag. Compatible with Gateway cloning. pDEST-periHisMBP 11086 Tac (lactose/IPTG...formation or prevent toxicity. Compatible with Gateway cloning. pGTvL1-SGC 39188 pTac (lactose/IPTG inducible...

  19. Zebrafish Plasmid Collection

    Type
    Collection
    ...Hai-Ning Du Lab. A set of pCS2+ backbone-based Gateway destination vectors (pGCS), bearing either amino...HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative Disorders ...
Showing: 21 - 40 of 61 results